Contents


Foreword    v

List of Previous Volumes    vi

Photographs of Some Symposium Participants    vii

List of Those Attending the Symposium    xi



REPLICATION OF NUCLEIC ACIDS

ENGLUND, P. T., M. P. DEUTSCHER, T. M. JOVIN, R. B. KELLY, N. R. COZZARELLI, and A. KORNBERG: Structural and functional properties of Eseherichia coli DNA polymerase    1

GOULIAN, M.: Initiation of the replication of single-stranded DNA by Eseherichia coli DNA polymerase    11

GELLERT, M., J. W. LITTLE, C. K. OSHINSKY, and S. B. ZIMMERMAN: Joining of DNA strands by DNA ligase of E. coli    21

OLIVERA, B. M., Z. W. HALL, Y. ANRAKU, J. R. CHIEN, and I. R. LEHMAN: On the mechanism of the polynucleotide joining reaction    27

KHORANA, H. G., H. BÜCHI, M. H. CARUTHERS, S. H. CHANG, N. K. GUPTA, A. KUMAR, E. OHTSUKA, V. SGARAMELLA, and H. WEBER: Progress in the total synthesis of the gene for ala-Trna    35

GANESAN, A. T.: Studies on in vitro replication of Bacillus subtilis DNA    45

BAUTZ, E. K. F., F. A. BAUTZ, and W. RPGER: A biological assay for in vitro repair of phage T4 DNA    59

ROSENBERG, B. H., and L. F. CAVALIERI: Shear sensitivity of the E. coli genome: Multiple membrane attachment points of the E. coli DNA    65

AUGUST, J. T., A. K. BANERJEE, L. EOYANG, M. T. F. DE FERNANDEZ, K. HORI, C. H. KUO, U. RENSING, and L. SHAPIRO: Synthesis of bacterio- phage Qb RNA    73

WEISSMANN, C., G. FEIX, H. SLOR: In vitro synthesis of phage RNA: the nature of the intermediates    83

SPIEGELMAN, S., N. R. PACE, D. R. MILLS, R. LEVISOHN, T. S. EIKHOM, M. M. TAYLOR, R. L. PETERSON, and D. H. L. BlSHOP: The mechanism of RNA replication    101

MARINO, P., M. I. BALDI, and G. P. TOCCHINI-VALENTINI: Effect of Rifampicin on DNA-dependent RNA polymerase and on RNA phage growth    125



INTERMEDIATES IN DNA SYNTHESIS

OKAZAKI, R., T. OKAZAKI, K. SAKABE, K. SUGIMOTO, R. KAINUMA, A. SUGINO, and N. IWATSUKI: In vivo mechanism of DNA chain growth    129

NEWMAN, J., and P. HANAWALT: Intermediates in T4 DNA replication in a T4 ligase deficient strain    145

RICHARDSON, C. C., Y. MASAMUNE, T. R. LIVE, A. JACQUEMIN-SABLON, B. WEISS, and G. FAREED: Studies on the joining of DNA by polynucleotide ligase of phage T4    151

SADOWSKI, P., B. GINSBERG, A. YUDELEVICH, L. FEINER, and J. HURWITZ: Enzymatic mechanisms of the repair and breakage of DNA    165

KIDSON, C.: Analysis of DNA fine secondary structure in relation to replication and transcription    179



REPAIR OF DNA

HANAWALT, P. C., D. E. PETTIJOHN, E. C. PAULING, C. F. BRUNK, D. W. SMITH, L. C. KANNER, and J. L. COUCH: Repair replication of DNA in vivo    187

HOWARD-FLANDERS, P., W. D. RUPP, B. M. WILKINS, and R. S. COLE: DNA replication and recombination after UV-irradiation    195

SETLOW, J. K., M. L. RANDOLPH, M. E. BOLING, A. MATTINGLY, G. PRICE, and M. P. GORDON: Repair of DNA in Haemophilus influenzae. II. Excision, repair of single-strand breaks, defects in transformation and host-cell modification in UV-sensitive mutants    209

TAKAGI, Y., M. SEKIGUCHI, S. OKUBO, H. NAKAYAMA, K. SHIMADA, S. YASUDA, T. MISHIMOTO, and H. YOSHIHARA: Nucleases specific for ultraviolet light- irradiated DNA and their possible role in dark repair    219

GROSSMAN, L., J. KAPLAN, S. KUSHNER, and I. MAHLER: Enzymes involved in the early stages of repair of ultraviolet-irradiated DNA    229

GANESAN, A. K., and K. C. SMITH: Recovery of recombination-deficient mutants of Escherichia coli K-12 from ultraviolet irradiation    235

TOMIZAWA, J., and H. OGAWA: Breakage of DNA in Rec+ and Rec- bacteria by disintegration of radiophosphorus atoms in DNA and possible causes of pleiotropic effects of RecA mutation    243

STEINBERG, R. A.: Effects of 32P-decay on chromosome transfer by a radiation- sensitive Hfr strain of Escherichia coli    253

BUTTIN, G., and M. R. WRIGHT: Enzymatic DNA degradation in E. coli: Its relationship to synthetic processes at the chromosome level    259

FRIEDBERG, E. C., and D. A. GOLDTHWAIT. Endonuclease II of E. coli    271

STRAUSS, B M. COYLE, and M. ROBBINS: Alkylation damage and its repair    277

ALBERTS, B. M., F. J. AMODIO, M. JENKINS, E. D. GUTMAN, and F. L. FERRIS: Studies with DNA-cellulose chromatography. I. DNA-binding proteins from E. coli    289



GENETICS OF REPLICATION

GROSS, J. D., D. KARAMATA, and P. G. HEMPSTEAD: Temperature-sensitive mutants of B. subtilis defective in DNA synthesis    307

MENDELSON, N. H.: Can defective segregation prevent initiation?    313

KOHLYAMA, M.: DNA synthesis in temperature-sensitive mutants of Escherichia coli    317

BERNSTEIN, H.: Repair and recombination in phage T4. I. Genes affecting recombination    325

BALDY, M. W.: Repair and recombination in phage T4. II. Genes affecting UV sensitivity    333

DRAKE, J. W., and E. F. ALLEN: Antimutagenic DNA polymerases of bacterio- phage T4    339

SPEYER, J. F., and D. ROSENBERG: The function of T4 DNA polymerase    345

CHIU, C-S, and G. R. GREENBERG: Evidence for a possible direct role of dCMP hydroxymethylase in T4 phage DNA synthesis    351

MOSIG, G., R. EHRING, and E. O. DUERE: Replication and recombination of DNA fragments in bacteriophage T4    361

AMATI, P., and R. FAVEE: Phage DNA synthesis in bacteria infected with T4 light particles    371

KOZINSKI, A. W.: Molecular recombination in the ligase-negative T4 amber mutant    375

SESNOWITZ-HORN, S., and E. A. ADELBERG: Profiavin treatment of Escherichia coli: Generation of frameshift mutations    393



STRUCTURE OF REPLICATING DNA

DAVISON, P. F.: DNA replication: Physical factors    403

WANG, J. C., and N. DAVIDSON: Cyclization of phage DNAs    409

THOMAS, C. A., JR., T. KELLY, JR., and M. RHOADES: The intracellular forms of T7 and P22 DNA molecules    417

FREIFELDER, D.: Studies with Escherichia coli sex factors    425

HUDSON, B., D. A. CLAYTON, and J. VINOGRAD: Complex mitochondrial DNA    435

SINSHEIMER, R. L., R. KNIPPERS, and T. KOMANO: Stages in the replication of bacteriophage ØX174 DNA in vivo    443

DENHARDT, D. T., and A. B. BURGESS: DNA replication in vitro. I. Synthesis of single-stranded ØX174 DNA in crude lysates of ØX-infected E. coli    449

RUSH, M. G., and R. C. WARNER: Molecular recombination ma circular genome- ØX174 and S13    459

HSU, Y.: Regulation of the propagation of HR virus plasmid    467

GILBERT, W., and D. DRESSLER: DNA replication: the rolling circle model    473

FRANKEL, F. R.: DNA replication after T4 infection    485

FRANKEL, F. R., C. MAJUMDAR, S. WEINTRAUB, and D. M. FRANKEL: DNA polymerase and the cell membrane after T4 infection    495

WERNER, R.: Initiation and propagation of growing points in DNA of phage T4    501

HUBERMAN, J.: Visualization of replicating mammalian and T4 bacteriophage DNA    509

WEISSBACH, A., P. BARTL, and L. A. SALZMAN: The structure of replicative lambda DNA-electron microscope studies    525

TOMIZAWA, J., and T. OGAWA: Replication of phage lambda DNA    533



ORIGINS OF REPLICATION

NAGATA, T., and M. MESELSON: Periodic replication of DNA in steadily growing E. coli: the localized origin of replication    553

CARO, L. G., and C. M. BERG: Chromosome replication in some strains of Escherichia coli K12    559

WOLF, B., M. L. PATO, C. B. WARD, and D. A. GLASER: On the origin and direction of replication of the E. coli chromosome    575

VIELMETTER, W., W. MESSER, and A. SCHUTTE: Growth direction and segregation of the E. coli chromosome    585

CERDÁ-OLMEDA, E., and P. HANAWALT: The replication of Escherichia coli chromosome studied by sequential nitrosoguanadine mutagenesis    599

LEPECQ, J.-B., and R. L. BALDWIN: The starting point and direction of lDNA replication    609

MAKOVER, S.: A preferred origin for the replication of lambda DNA    621



BACTERIAL MATING

BONHOEFFER, F., and W. VIELMETTER: Conjugational DNA transfer in E. coli    623

FISHER, K. W., and M. B. FISHER: Nalidixic acid inhibition of DNA transfer in E. coli K12    629

COHEN, A., W. D. FISHER, R. CURTISS, III, and H. I. ADLER: The properties of DNA transferred to minicells during conjugation    635

GUILD, W. R., A. CATO, JR., and S. LACKS: Transformation and DNA size: two controlling parameters and the efficiency of the single strand intermediates    643

RUPP, W. D., and G. IHLER: Strand selection during bacterial mating    647

OHKI, M., and J. TOMIZAWA: Asymmetric transfer of DNA strands in bacterial conjugation    651



REPLICATION AND THE CELL MEMBRANE

BOTSTEIN, D., and M. LEVINE: Intermediates in the synthesis of phage P22 DNA    659

RYTER, A., Y. HIROTA, and F. JACOB: DNA-membrane complex and nuclear segregation in bacteria    669

HIROTA, Y., A. RYTER, and F. JACOB: Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division    677

SUEOKA, N., and W. G. QUINN: Membrane attachment of the chromosome replication origin in Bacillus subtilis    695

EARHART, F., G. Y. TREMBLAY, M. J. DANIELS, and M. SCHAECHTER: DNA repli cation studied by a new method for the isolation of cell membrane-DNA complexes    707



REPLICATION OF TEMPERATE VIRUSES

SIGNER, E., H. ECHOLS, J. WEIL, C. RADDING, M. SHULMAN, L. MOORE, and K. MANLY: The general recombination system of bacteriophage l    711

SIGNER, E. and J. WElL: Site-specific recombination of bacteriophage l    715

GINGERY, R., and H. ECHOLS: Integration, excision, and transducing particle genesis by bacteriophage l    721

KAISER, A. D., and R. WU: Structure and function of DNA cohesive ends    729

GOTTESMAN, M., and M. YARMOLINSKY: The integration and excision of the bacteriophage lambda genome    735

MOUSSET, S., and R. THOMAS: Dilysogenic excision: an accessory expression of the termination function?    749

EISEN, H., L. H. PEREIRA DA SILVA, and F. JACOB: The regulation and mech- anism of DNA synthesis in bacteriophage lambda    755

CALEF, E., and Z. NEUBAUER: Active and inactive states of the CI gene in some l defective phages    765

MATSUBARA, K., and A. D. KAISER: l dv, an autonomously replicating DNA fragment    769

DULBECCO, R.: The state of the DNA of polyoma virus and 5V40 in transformed cells    777

ROSNER, J. L., L. R. KASS, and M. B. YARMOLINSKY: Parallel behavior of F and P1 in causing indirect induction of lysogenic bacteria    785

IKEDA, H., and J. TOMIZAWA: Prophage P1, an extrachromosomal replication unit    791



REGULATION OF REPLICATION

BIRD, R., and K. G. LARK: Initiation and termination of DNA replication after amino acid starvation of E. coli 15T-    799

HELMSTETTER, C. E., S. COOPER, O. PIERUCCI, and E. REVELAS: On the bacterial life sequence    809

CLARK, D. J.: The regulation of DNA replication and cell division in E. coli B/r    823

SGARAMELLA, V., S. SPADARI, and A. FALASCHI: Isolation of the hybrid between ribosomal RNA and DNA of Bacillus subtilis    839

YOSHIKAWA, H., and M. HAAS: On the regulation of the initiation of DNA replication in bacteria    843



SUMMARY

HOTCHKISS, R.: Metabolism and growth of gene substance: 1968    857