Organizers: Mark Adams, Nigel Brown, Mila Pollock & Robert Waterston

Session 1: Early Days

Chair: Robert Waterston,     University of Washington

George Brownlee
The early days of RNA sequencing at the LMB
James D. Watson
Early Days with DNA
Gillian Air
Integration of protein & DNA sequencing for PhiX174
Clyde Hutchison
Sequencing of PhiX174
Walter Gilbert
Origin of DNA Sequencing
Tom Maniatis
The transition from RNA to DNA sequencing in the Sanger lab:
The DNA sequence of the phage lambda operator/promoter regions
Joachim Messing
Development of M13 cloning systems for sequencing

Session 2A: Capturing Sequences

Chair: Mila Pollock,     Cold Spring Harbor Laboratory

Lee Hood
Automation of Sanger Sequencing
Llyod Smith
Fluorescence-based automated DNA Sequencing
Norman Dovichi
Development of capillary electrophoresis
Mostafa Ronaghi
Development of pyrosequencing

Session 2B: Capturing Sequences

Chair: Miguel Garcia-Sancho,     University of Edinburgh

Shankar Balasubramanian
Early development of Solexa technology-key insights & technical breakthroughs
Jonas Korlach
Technical innovations of SMRT Sequencing and applications of long-read sequencing
Hagan Bayley
Nanopore Sequencing

Session 3: Access to Sequence from the past to the future

Chair: Robert Waterston,     University of Washington

David Lipman
Origins of GenBank
Graham Cameron
DNA database prehistory
Jim Ostell
Databases for the future
Access to Sequences Q&A
Miguel Garcia-Sancho
Sequencing & computing technologies: a Historical Convergence
Mila Pollock
Genome legacy (preserving the history)

Session 4: Scaling to Genomes

Chair: Mark Adams,     J. Craig Venter Institute

Jean Weissenbach
Genoscope early efforts at automation
Stanley Tabor
How enzymology enabled advances in DNA sequencing
Melvin Simon
Large insert cloning
William Efcavitch
Technology development in scaling up Sanger sequencing
Jane Rogers
Scaling up Sanger sequencing in the genome era
Richard Myers
A personal perspective on DNA sequencing from 1978 to 2015
Yoshiyuki Sakaki
From the proposal of automated DNA sequencing to the completion of the Human Genome:
Japanese contribution to Human Genome sequencing

Session 5: Sequences to Genomes

Chair: Richard Roberts,     New England BioLabs

J. Craig Venter
Whole genome shotgun sequencing
Hamilton Smith
Haemophilus influenzae and the value of completeness
Philip Green
Sequence quality & assembly
James Kent
Integrating the Sequence & Map into a genome
Gene Myers
Shotgun assembly strategies
Suzanna Lewis
Making sense of genomes with visualization and collaboration

Session 6: All Roads Lead to DNA: Beyond Genomes

Chair: Jane Rogers,     International Wheat Genome Sequencing Consortium, UK

Mark Adams
Sequencing ESTs for gene discovery
Barbara Wold
Developments & Applications of RNA-seq
Jack Gilbert
Metagenomic Sequencing
Piero Carninci
cDNA Sequencing for genome analysis & biological interpretation
Jay Shendure
Novel applications of DNA sequencing
Victor Ling
Fractionation & sequences of large pyrimidine oligonucleotides—1970-71

Panel: Steps and mis-steps during the development of sequencing technologies

Steps and mis-steps during the development of sequencing technologies
Richard McCombie
Panel Discussion
Richard Roberts
Panel Discussion
Cheryl Heiner
Panel Discussion

Session 7: Human Variation and Disease

Chair: Barbara Wold,     CalTech

Robert Waterston
C. elegans: How complete can we get?
Huanming Yang
China: a latecomer to the global sequencing effort
Debbie Nickerson
Sequencing in human genetics
Mark Gerstein
David Bentley
Genomes for Medicine
Jim Lupski
Applications of sequencing in clinical genetics