Program
Organizers: Mark Adams, Nigel Brown, Mila Pollock & Robert Waterston
Session 1: Early Days
Chair: Robert Waterston, University of Washington
- George Brownlee
- The early days of RNA sequencing at the LMB
- James D. Watson
- Early Days with DNA
- Gillian Air
- Integration of protein & DNA sequencing for PhiX174
- Clyde Hutchison
- Sequencing of PhiX174
- Walter Gilbert
- Origin of DNA Sequencing
- Tom Maniatis
- The transition from RNA to DNA sequencing in the Sanger lab:
The DNA sequence of the phage lambda operator/promoter regions
- Joachim Messing
- Development of M13 cloning systems for sequencing
Session 2A: Capturing Sequences
Chair: Mila Pollock, Cold Spring Harbor Laboratory
- Lee Hood
- Automation of Sanger Sequencing
- Llyod Smith
- Fluorescence-based automated DNA Sequencing
- Norman Dovichi
- Development of capillary electrophoresis
- Mostafa Ronaghi
- Development of pyrosequencing
Session 2B: Capturing Sequences
Chair: Miguel Garcia-Sancho, University of Edinburgh
- Shankar Balasubramanian
- Early development of Solexa technology-key insights & technical breakthroughs
- Jonas Korlach
- Technical innovations of SMRT Sequencing and applications of long-read sequencing
- Hagan Bayley
- Nanopore Sequencing
Session 3: Access to Sequence from the past to the future
Chair: Robert Waterston, University of Washington
- David Lipman
- Origins of GenBank
- Graham Cameron
- DNA database prehistory
- Jim Ostell
- Databases for the future
- Access to Sequences Q&A
- Miguel Garcia-Sancho
- Sequencing & computing technologies: a Historical Convergence
- Mila Pollock
- Genome legacy (preserving the history)
Session 4: Scaling to Genomes
Chair: Mark Adams, J. Craig Venter Institute
- Jean Weissenbach
- Genoscope early efforts at automation
- Stanley Tabor
- How enzymology enabled advances in DNA sequencing
- Melvin Simon
- Large insert cloning
- William Efcavitch
- Technology development in scaling up Sanger sequencing
- Jane Rogers
- Scaling up Sanger sequencing in the genome era
- Richard Myers
- A personal perspective on DNA sequencing from 1978 to 2015
- Yoshiyuki Sakaki
- From the proposal of automated DNA sequencing to the completion of the Human Genome:
Japanese contribution to Human Genome sequencing
Session 5: Sequences to Genomes
Chair: Richard Roberts, New England BioLabs
- J. Craig Venter
- Whole genome shotgun sequencing
- Hamilton Smith
- Haemophilus influenzae and the value of completeness
- Philip Green
- Sequence quality & assembly
- James Kent
- Integrating the Sequence & Map into a genome
- Gene Myers
- Shotgun assembly strategies
- Suzanna Lewis
- Making sense of genomes with visualization and collaboration
Session 6: All Roads Lead to DNA: Beyond Genomes
Chair: Jane Rogers, International Wheat Genome Sequencing Consortium, UK
- Mark Adams
- Sequencing ESTs for gene discovery
- Barbara Wold
- Developments & Applications of RNA-seq
- Jack Gilbert
- Metagenomic Sequencing
- Piero Carninci
- cDNA Sequencing for genome analysis & biological interpretation
- Jay Shendure
- Novel applications of DNA sequencing
- Victor Ling
- Fractionation & sequences of large pyrimidine oligonucleotides—1970-71
Panel: Steps and mis-steps during the development of sequencing technologies
- Steps and mis-steps during the development of sequencing technologies
- Richard McCombie
- Panel Discussion
- Richard Roberts
- Panel Discussion
- Cheryl Heiner
- Panel Discussion
Session 7: Human Variation and Disease
Chair: Barbara Wold, CalTech
- Robert Waterston
- C. elegans: How complete can we get?
- Huanming Yang
- China: a latecomer to the global sequencing effort
- Debbie Nickerson
- Sequencing in human genetics
- Mark Gerstein
- ENCODE
- David Bentley
- Genomes for Medicine
- Jim Lupski
- Applications of sequencing in clinical genetics
