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 XLIX: Recombination at the DNA Level 1984 |
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From left: Dr. Anna Marie Skalka, Dr. Alfred Hershey  SYMPOSIUM SYNOPSIS LIST OF PARTICIPANTS CONTENTS OF SYMPOSIUM VOLUME PHOTOGRAPHS PUBLISHED VOLUME  |
Recombination at the DNA Level
Symposium Volume XLIX Contents
Symposium Participants vii
Foreword xix
Introduction
The DNA Enzymology of Protein Machines B.M. Alberts 1
Chromosomal Mechanics
Origins of Gene Conversion and Reciprocal Exchange in Ascobolus J.-L. Rossignol, A. Nicholas, H. Hamza, and T. Langin 13
Meiotic Roles of Crossing-over and of Gene Conversion A. T. C. Carpenter 23
Interallelic and Intergenic Conversion in Three Serine tRNA Genes of Schizosac- charomyces pombe J. Kohli, P. Munz, R. Aebi, H. Amstutz, C. Gysler, W. -D. Heyer, L. Lehmann, P. Schuchert, P. Szankasi, P. Thuriaux, U. Leupold, J. Bell, V. Gamulin, H. Hottinger, D. Pearson, and D. Söll 31
Recombination in Saceharomyces cerevisiae: REC-gene Mutants and DNA-binding Proteins M. S. Esposito, J. Hosoda, J. Golin, H. Moise, K. Bjornstad, and D. Maleas 41
Measurement of Restoration and Conversion: Its Meaning for the Mismatch Repair Hypothesis of Conversion P. J. Hastings 49
Yeast Systems
Meiotic Gene Conversion Mediates Gene Amplification in Yeast S. Fogel, J. W. Welch, and E.J. Louis 55
Physical Monitoring of Meiotic Recombination in Saccharomyces cerevisiae R.H. Borts, M. Lichten, M. Hearn, L.S. Davidow, and J.E. Haber 67
Involvement of Double-strand Chromosomal Breaks for Mating-type Switching in Saccharomyces cerevisiae A.J.S. Klar, J.N. Strathern, and J.A. Abraham 77
The Product of the HO Gene Is a Nuclease: Purification and Characterization of the Enzyme R. Kostriken and F. Heifron 89
Directionality and Regulation of Cassette Substitution in Yeast R.E. Jensen and I. Herskowitz 97
The Role of DNA Replication in the Repression of the Yeast Mating-type Silent Loci A.M. Miller, R. Sternglanz, and K.A. Nasmyth 105
Kinetics and Intermediates of Yeast Mitochondrial DNA Recombination A.R. Zinn and R.A. Butow 115
Mammalian Homologous Recombination
Analysis of Homologous Recombination in Cultured Mammalian Cells K. Folger, K. Thomas, and M.R. Capecchi 123
Homologous Recombination in Mouse L Cells F.-L. Lin, K. Sperle, and N. Stern- berg 139
The Recombination and Integration of DNAs Introduced into Mouse L Cells D.A. Brenner, S. Kato, R.A. Anderson, A.C. Smigocki, and R.D. Camerini-Otero 151
Homologous Recombination with DNA Introduced into Mammalian Cells O. Smi- thies, M.A. Koralewski, K.-Y. Song, and R.S. Kucherlapati 161
Homologous Recombination between Defective neo Genes in Mouse 3T6 Cells A.J.H. Smith and P. Berg 171
Homologous Recombination between Repeated Chromosomal Sequences in Mouse Cells R.M. Liskay, J.L. Stachelek, and A. Letsou 183
Homologous Recombination in Monkey Cells and Human Cell-free Extracts R. S. Kucherlapati, D. Ayares, A. Hanneken, K. Noonan, S. Rauth, J.M. Spencer, L. Wallace, and P.D. Moore 191
Transposons
Origin of Cryptic l Prophages in Escherichia coli K-12 R.J. Redfield and A.M. Campbell 199
Functional Difference of the Two Ends of Insertion Sequence IS1 in Transposition and Cointegration K. Ishizaki and E. Ohtsubo 207
Transposon Tn5: Specific Sequence Recognition and Conservative Transposition D.E. Berg, J. Lodge, C. Sasakawa, D.K. Nag, S.H. Phadn is, K. Weston-Hafer, and G.F. Cane 215
Site-specific Recombination in Transposition and Plasmid Stability D. Sherratt, P. Dyson, M. Boocock, L. Brown, D. Summers, G. Stewart, and P. Chan 227
Mechanism and Regulation of Tnl0 Transposition N. Kieckner, D. Morisato, D. Roberts, and J. Bender 235
Resolvase-mediated Recombination Intermediates Contain a Serine Residue Co- valently Linked to DNA R.R. Reed and C.D. Moser 245
Replicative and Conservative Transpositional Recombination of the Insertion Sequences T.A. Weinert, K. Derbyshire, F.M. Hughson, and N.D.F. Grindley 251
Mu
Mapping and Properties of the gam and sot Genes of Phage Mu: Their Possible Roles in Recombination J. Akroyd, B. Barton, P. Lund, S. Maynard Smith, K. Sul tana, and N. Symonds 261
Analysis of the Regulation of the Transposition Functions of Bacteriophage Mu by Using Gene Fusions T.A. Patterson, K.A. Martin, and A.I. Bukhari 267
Nonreplicative DNA Transposition: Integration of Infecting Bacteriophage Mu R.M. Harshey 273
Transposition of Bacteriophage Mu DNA: Expression of the A and B Proteins from lPL and Analysis of Infecting Mu DNA G. Chaconas, G. Gloor, J.L. Miller, D.L. Kennedy, E.B. Giddens, and C.R. Nagainis 279
Substrate and Enzyme Requirements for In Vitro Site-specific Recombination in Bac- teriophage Mu R. Kahmann, F. Rudt, and G. Mertens 285
Inversion of DNA In Vivo and In Vitro by Gin and Pin Proteins R.H.A. Plasterk and P. van de Putte 295
Comparative Analysis of Invertible DNA in Phage Genomes D. Kamp, E. Kardas, W. Ritthaler, R. Sandulache, R. Schumucker, and B. Stern 301
Programming of DNA Rearrangements Involving Mu Phrophages J.A. Shapiro and P.M. Brinkley 313
Plant Transposons/T4 Recombination
Endogenous Transposable Elements Associated with Virus Infection in Maize S.L. Dellaporta, P.S. Chomet, J.P. Mottinger, J.A. Wood, S.-M. Yu, and J.B. Hicks 321
Transposable Elements Ac and Ds at the Shrunken, Waxy, and Alcohol Dehydroge- nase 1 Loci in Zea mays L U. Courage, H.-P. Döring, W.-B. Frommer, R. Kunze, Laird, A. Merckelbach, M. Muller-Neumann, J. Riegel, P. Starlinger, E. Till- mann, E. Weck, W. Werr, and J. Yoder 329
Isolation of Spin Controlling Elements from Maize N. Fedoroff, M. Shure, S. Kelly, M. Johns, D. Furtek, J. Schiefelbein, and O. Nelson 339
Insertion and Excision of Ds Controlling Elements in Maize W. J. Peacock, E. S. Dennis, W.L. Gerlach, M.M. Sachs, and D. Schwartz 347
Transposable Elements in Antirrhinum majus and Zea mays H. Saedler, U. Bonas, A. Gierl, B.J. Harrison, R.B. Klösgen, E. Krebbers, P. Nevers, P.A. Peterson, Z. Schwarz-Sommer, H. Sommer, K. Upadhyaya, and U. Wienand 355
The Use of Affinity Chromatography to Study Proteins Involved in Bacteriophage T4 Genetic Recombination T. Formosa and B.M. Alberts 363
On the Role of DNA Replication, Endonuclease VII, and rII Proteins in Processing of Recombinational Intermediates in Phage T4 G. Mosig, M. Shaw, and G.M. Garcia 371
Topoisomerase, Resolvase, and Gyrase
A Topological Treatment of Recombination and Topoisomerases N.R. Cozzarelli, M.A. Krasnow, S.P. Gerrard, and J.H. White 383
Nonhomologous Recombination Mediated by Escherichia coli DNA Gyrase: Possible Involvement of DNA Replication H. Ikeda and M. Shiozaki 401
The Cleavage of DNA by Type-I DNA Topoisomerases K. Kirkegaard, G. Pflug- felder, and J. C. Wang 411
Site-specific Recombination in the oriV1 Region of the F Factor M.B. O'Connor and M.H. Malamy 421
DNA Structural Features That Lead to Strand Breakage by Eukaryotic Type-I Topoisomerase J.J. Champoux, W.K. McCoubrey, Jr., and M.D. Been 435
Escherichia coli General Recombination
Host Functions in Amplification/Deamplification of Tn9 in Escherichia coli K-12: A New Model for Amplification S.K. Mahajan, N.N. Pandit, and J.F. Sarkari 443
Genes of the RecE and RecF Pathways of Conjugational Recombination in Esche- richia coli A.J. Clark, S.J. Sandler, K.D. Willis, C.C. Chu, M.A. Blanar, and S. T. Lovett 453
Purification and Characterization of Exonuclease V from Escherichia coli K- 12 C. C. Dykstra, K.M. Palas, and S.R. Kushner 463
Genetic Analysis and Regulation of Inducible Recombination in Escherichia coli K- 12 S.M. Picksley, R.G. Lloyd, and C. Buckman 469
RecF and RecBC Function during Recombination of Nonreplicating, UV-irradiated Phage l DNA and during Other Recombination Processes J.B. Hays, T.A. G. Smith, S.A. Friedman, F. Lee, and G.L. Coffman 475
Roles of RecBC Enzyme and Chi Sites in Homologous Recombination G.R. Smith, S.K. Amundsen, A.M. Chaudhury, K.C. Cheng, A.S. Ponticelli, C.M. Roberts, D. W. Schultz, and A.F. Taylor 485
The Mechanism of the Chi-cos Interaction in RecA-RecBC-mediated Recombination in Phage l I. Kobayashi, M.M. Stahl, and F. W. Stahl 497
RecA
A Model for the Core Structure of the Escherichia coli RecA Protein M.A. Blanar, D. Kneller, A.J. Clark, A.E. Karu, F.E. Cohen, R. Lan gridge, and I.D. Kuntz 507
Intermediates in Homologous Pairing Promoted by RecA Protein and Correlations of Recombination In Vitro and In Vivo S.S. Flory, J. Tsang, K. Muniyappa, M. Bianchi, D. Gonda, R. Kahn, F. Azhderian, C. Egner, S. Shaner, and C.M Radding 513
Unidirectional Branch Migration Promoted by Nucleoprotein Filaments of RecA Pro- tein and DNA M.M. Cox, S. W. Morrical, and S.K. Neuendorf 525
Studies of the Mechanism of DNA Pairing by the RecA Protein of Escherichia coli F.R. Bryant, P. W. Riddles, and I.R. Lehman 535
Roles of Processive Unwinding in Recombination Reactions Promoted by RecA Pro- tein of Escherichia coli: A Study Using a Monoclonal Antibody T. Shibata, O. Makino, S. Ikawa, T. Ohtani, M. Iwabuchi, Y. Shibata, H. Maeda, and T. Ando 541
Visualization of SSB-ssDNA Complexes Active in the Assembly of Stable RecA-DNA Filaments J.D. Griffith, L.D. Harris, and J. Register III 553
Visualization of RecA-DNA Complexes Involved in Consecutive Stages of an In Vitro Strand Exchange Reaction A. Stasiak, A. Z. Stasiak, and T. Koller 561
Molecular Mechanisms of General Genetic Recombination: The DNA-binding Sites of the RecA Protein P. Howard-Flanders, S.C. West, J.R. Rusche, and E.H. Egel- man 571
Repair
Studies of HeLa DNA Polymerases during Excision-repair In Vitro with DNA and Chromatin Substrates D. W. Mosbaugh, D.H. Evans, and S. Linn 581
Repair of DNA Base-pair Mismatches in Extracts of Escherichia coli A -L. Lu, K. Welsh, S. Clark, S.-S. Su, and P. Modrich 589
The mutH, mutL, mutS, and uvrD Genes of Salmonella typhimurium LT2 P.P. Pang, S. -D. Tsen, A .S. Lundberg, and G. C. Walker 597
An Escherichia coli Cell-free System That Catalyzes the Repair of Symmetrically Methylated Heteroduplex DNA R.A. Fishel and R. Kolodner Involvement of Escherichia coil Mismatch Repair in DNA Replication and Recombination R. Wagner, C. Dohet, M. Jones, M. -P. Doutriaux, F. Hutch- inson, and M. Radman 611
Formation of Inverted Dimer Plasmids after Transformation of Yeast with Linearized Plasmid DNA S. Kunes, D. Botstein, and M. S. Fox 617
Double-strand-break Repair, Gene Conversion, and Postdivision Segregation R. Roth- stein 629
Eukaryotic Enzymes
Changes in the Chromosomal DNA of Yeast during Meiosis in Repair Mutants and the Possible Role of a Deoxyribonuclease M.A. Resnick, T. Chow, J. Nitiss, and J. Game 639
Integrative Recombination-A Role for the Retroviral Reverse Transcriptase A.M. Skalka, G. Duyk, M. Longiaru, P. DeHaseth, R. Terry, and J. Leis 651
Endonuclease J: A site-specific Endonuclease Cleaving Immunoglobulin Genes S. Kondo, T. Kataoka, M. Nishi, M. Kodaira, S. Takeda, and T. Honjo 661
Biochemical Characterization of recI Mutants and the Genetics Control of Recombi- nation in Ustilago maydis R. Holliday, S. Y. Taylor, E.B. Kmiec, and W.K. Hol- loman 669
Homologous Pairing Promoted by Ustilago Red Protein F. Kmiec, P. Kroeger, R. Holliday, and W. Holloman 675
Poly(ADP-ribosylation) of DNA Topoisomerase I: A Nuclear Response to DNA-strand Interruptions A .M. Ferro, M. C. McElwain, and B.M. Olivera 683
Integration and Excision of Bacteriophage
Primary Structure and the himA Gene of Escherichia coli: Homology with DNA-bind- ing Protein HU and Association with the Phenylalanyl-tRNA Synthetase Operon H.I. Miller 691
DNA Interactions during Bacteriophage l Site-Specific Recombination C.E. Bauer, S.D. Hesse, J.F. Gardner, and R.I. Gumport 699
Site-specific Recombination Systems of Phages f 80 and P22: Binding Sites of Integra- tion Host Factor and Recombination-induced Mutations J. Leong, S. Nunes- Duby, A. Oser, C. Lesser, P. Youderian, M.M. Susskind, and A. Landy 707
Enzymes and Sites of Genetic Recombination: Studies with Gene-3 Endonuclease of Phage T7 and with Site-affinity Mutants of Phage l B. de Massy, F. W. Studier, L. Dorgai, E. Appelbaum, and R.A. Weisberg 715
The Role of Specialized Nucleoprotein Structures in Site-specific Recombination and Initiation of DNA Replication H. Echo Is, M. Dodson, M. Better, J.D. Roberts, and R. McMacken 727
l Integrative Recombination: Supercoiling, Synapsis, and Strand Exchange P. Kitts, E. Richet, and H.A. Nash 735
Quantitative Analysis of the Contributions of Enzyme and DNA to the Structure of l Integrative Recombinants S.J. Spengler, A. Stasiak, A.Z. Stasiak, and N.R. Cozzarelli 745
Site-specific Recombination
In Vitro Analysis of Hin-mediated Site-specific Recombination R. C. Johnson, M.B. Bruist, M.B. Glaccum, and M.L Simon 751
The Nature of the Interaction of the P1 Recombinase Cre with the Recombining Site loxP R. Hoess, K. Abremski, and N. Sternberg 761
The Bacteriophage P1 Site-specific Recombinase Cm: Recombination Events and DNA Recognition Sequences S. Jida, H. Huber, R. Hiestand-Nauer, J. Meyer, T.A. Bickle, and W. Arber 769
Components of the Site-specific Recombination System Encoded by the Yeast Plasmid 2-micron Circle M. McLeod, F. Volkert, and J. Broach 779
In Vitro Systems for Genetic Recombination of the DNAs of Bacteriophage T7 and Yeast 2-micron Circle P.D. Sadowski, D.D. Lee, B.J. Andrews, D. Babineau, L. Beatty, M.J. Morse, G. Proteau, and D. Vetter 789
Site-Specific Genetic Recombination Promoted by the FLP Protein of the Yeast 2- micron Plasmid In Vitro L. Meyer-Leon, J.F. Senecoff, R. C. Bruckner, and M.M. Cox 797
Recombination in Vitro
Genetic Recombination Catalyzed by Cell-free Extracts of Saccharomyces cerevisiae L.S. Symington, P. T. Morrison, and R. Kolodner 805
Resolution of Holliday Structures by Endonuclease VII As Observed in Interactions with Cruciform DNA B. Kemper, F. Jensch, M. v. Depka-Prondzynski, H.-J. Fritz, U. Borgmeyer, and K. Mizuuchi 815
On the Mu Repressor and Early DNA Intermediates of Transposition H.M. Krause and N.P. Higgins 827
The Mechanism of Transposition of Bacteriophage Mu K. Mizuuchi, M. Mizuuchi, and R.. Craigie 835
Summary
Types of Recombination: Common Problems and Common Strategies A. Campbell 839
Foreword xix
Introduction
The DNA Enzymology of Protein Machines B.M. Alberts 1
Chromosomal Mechanics
Origins of Gene Conversion and Reciprocal Exchange in Ascobolus J.-L. Rossignol, A. Nicholas, H. Hamza, and T. Langin 13
Meiotic Roles of Crossing-over and of Gene Conversion A. T. C. Carpenter 23
Interallelic and Intergenic Conversion in Three Serine tRNA Genes of Schizosac- charomyces pombe J. Kohli, P. Munz, R. Aebi, H. Amstutz, C. Gysler, W. -D. Heyer, L. Lehmann, P. Schuchert, P. Szankasi, P. Thuriaux, U. Leupold, J. Bell, V. Gamulin, H. Hottinger, D. Pearson, and D. Söll 31
Recombination in Saceharomyces cerevisiae: REC-gene Mutants and DNA-binding Proteins M. S. Esposito, J. Hosoda, J. Golin, H. Moise, K. Bjornstad, and D. Maleas 41
Measurement of Restoration and Conversion: Its Meaning for the Mismatch Repair Hypothesis of Conversion P. J. Hastings 49
Yeast Systems
Meiotic Gene Conversion Mediates Gene Amplification in Yeast S. Fogel, J. W. Welch, and E.J. Louis 55
Physical Monitoring of Meiotic Recombination in Saccharomyces cerevisiae R.H. Borts, M. Lichten, M. Hearn, L.S. Davidow, and J.E. Haber 67
Involvement of Double-strand Chromosomal Breaks for Mating-type Switching in Saccharomyces cerevisiae A.J.S. Klar, J.N. Strathern, and J.A. Abraham 77
The Product of the HO Gene Is a Nuclease: Purification and Characterization of the Enzyme R. Kostriken and F. Heifron 89
Directionality and Regulation of Cassette Substitution in Yeast R.E. Jensen and I. Herskowitz 97
The Role of DNA Replication in the Repression of the Yeast Mating-type Silent Loci A.M. Miller, R. Sternglanz, and K.A. Nasmyth 105
Kinetics and Intermediates of Yeast Mitochondrial DNA Recombination A.R. Zinn and R.A. Butow 115
Mammalian Homologous Recombination
Analysis of Homologous Recombination in Cultured Mammalian Cells K. Folger, K. Thomas, and M.R. Capecchi 123
Homologous Recombination in Mouse L Cells F.-L. Lin, K. Sperle, and N. Stern- berg 139
The Recombination and Integration of DNAs Introduced into Mouse L Cells D.A. Brenner, S. Kato, R.A. Anderson, A.C. Smigocki, and R.D. Camerini-Otero 151
Homologous Recombination with DNA Introduced into Mammalian Cells O. Smi- thies, M.A. Koralewski, K.-Y. Song, and R.S. Kucherlapati 161
Homologous Recombination between Defective neo Genes in Mouse 3T6 Cells A.J.H. Smith and P. Berg 171
Homologous Recombination between Repeated Chromosomal Sequences in Mouse Cells R.M. Liskay, J.L. Stachelek, and A. Letsou 183
Homologous Recombination in Monkey Cells and Human Cell-free Extracts R. S. Kucherlapati, D. Ayares, A. Hanneken, K. Noonan, S. Rauth, J.M. Spencer, L. Wallace, and P.D. Moore 191
Transposons
Origin of Cryptic l Prophages in Escherichia coli K-12 R.J. Redfield and A.M. Campbell 199
Functional Difference of the Two Ends of Insertion Sequence IS1 in Transposition and Cointegration K. Ishizaki and E. Ohtsubo 207
Transposon Tn5: Specific Sequence Recognition and Conservative Transposition D.E. Berg, J. Lodge, C. Sasakawa, D.K. Nag, S.H. Phadn is, K. Weston-Hafer, and G.F. Cane 215
Site-specific Recombination in Transposition and Plasmid Stability D. Sherratt, P. Dyson, M. Boocock, L. Brown, D. Summers, G. Stewart, and P. Chan 227
Mechanism and Regulation of Tnl0 Transposition N. Kieckner, D. Morisato, D. Roberts, and J. Bender 235
Resolvase-mediated Recombination Intermediates Contain a Serine Residue Co- valently Linked to DNA R.R. Reed and C.D. Moser 245
Replicative and Conservative Transpositional Recombination of the Insertion Sequences T.A. Weinert, K. Derbyshire, F.M. Hughson, and N.D.F. Grindley 251
Mu
Mapping and Properties of the gam and sot Genes of Phage Mu: Their Possible Roles in Recombination J. Akroyd, B. Barton, P. Lund, S. Maynard Smith, K. Sul tana, and N. Symonds 261
Analysis of the Regulation of the Transposition Functions of Bacteriophage Mu by Using Gene Fusions T.A. Patterson, K.A. Martin, and A.I. Bukhari 267
Nonreplicative DNA Transposition: Integration of Infecting Bacteriophage Mu R.M. Harshey 273
Transposition of Bacteriophage Mu DNA: Expression of the A and B Proteins from lPL and Analysis of Infecting Mu DNA G. Chaconas, G. Gloor, J.L. Miller, D.L. Kennedy, E.B. Giddens, and C.R. Nagainis 279
Substrate and Enzyme Requirements for In Vitro Site-specific Recombination in Bac- teriophage Mu R. Kahmann, F. Rudt, and G. Mertens 285
Inversion of DNA In Vivo and In Vitro by Gin and Pin Proteins R.H.A. Plasterk and P. van de Putte 295
Comparative Analysis of Invertible DNA in Phage Genomes D. Kamp, E. Kardas, W. Ritthaler, R. Sandulache, R. Schumucker, and B. Stern 301
Programming of DNA Rearrangements Involving Mu Phrophages J.A. Shapiro and P.M. Brinkley 313
Plant Transposons/T4 Recombination
Endogenous Transposable Elements Associated with Virus Infection in Maize S.L. Dellaporta, P.S. Chomet, J.P. Mottinger, J.A. Wood, S.-M. Yu, and J.B. Hicks 321
Transposable Elements Ac and Ds at the Shrunken, Waxy, and Alcohol Dehydroge- nase 1 Loci in Zea mays L U. Courage, H.-P. Döring, W.-B. Frommer, R. Kunze, Laird, A. Merckelbach, M. Muller-Neumann, J. Riegel, P. Starlinger, E. Till- mann, E. Weck, W. Werr, and J. Yoder 329
Isolation of Spin Controlling Elements from Maize N. Fedoroff, M. Shure, S. Kelly, M. Johns, D. Furtek, J. Schiefelbein, and O. Nelson 339
Insertion and Excision of Ds Controlling Elements in Maize W. J. Peacock, E. S. Dennis, W.L. Gerlach, M.M. Sachs, and D. Schwartz 347
Transposable Elements in Antirrhinum majus and Zea mays H. Saedler, U. Bonas, A. Gierl, B.J. Harrison, R.B. Klösgen, E. Krebbers, P. Nevers, P.A. Peterson, Z. Schwarz-Sommer, H. Sommer, K. Upadhyaya, and U. Wienand 355
The Use of Affinity Chromatography to Study Proteins Involved in Bacteriophage T4 Genetic Recombination T. Formosa and B.M. Alberts 363
On the Role of DNA Replication, Endonuclease VII, and rII Proteins in Processing of Recombinational Intermediates in Phage T4 G. Mosig, M. Shaw, and G.M. Garcia 371
Topoisomerase, Resolvase, and Gyrase
A Topological Treatment of Recombination and Topoisomerases N.R. Cozzarelli, M.A. Krasnow, S.P. Gerrard, and J.H. White 383
Nonhomologous Recombination Mediated by Escherichia coli DNA Gyrase: Possible Involvement of DNA Replication H. Ikeda and M. Shiozaki 401
The Cleavage of DNA by Type-I DNA Topoisomerases K. Kirkegaard, G. Pflug- felder, and J. C. Wang 411
Site-specific Recombination in the oriV1 Region of the F Factor M.B. O'Connor and M.H. Malamy 421
DNA Structural Features That Lead to Strand Breakage by Eukaryotic Type-I Topoisomerase J.J. Champoux, W.K. McCoubrey, Jr., and M.D. Been 435
Escherichia coli General Recombination
Host Functions in Amplification/Deamplification of Tn9 in Escherichia coli K-12: A New Model for Amplification S.K. Mahajan, N.N. Pandit, and J.F. Sarkari 443
Genes of the RecE and RecF Pathways of Conjugational Recombination in Esche- richia coli A.J. Clark, S.J. Sandler, K.D. Willis, C.C. Chu, M.A. Blanar, and S. T. Lovett 453
Purification and Characterization of Exonuclease V from Escherichia coli K- 12 C. C. Dykstra, K.M. Palas, and S.R. Kushner 463
Genetic Analysis and Regulation of Inducible Recombination in Escherichia coli K- 12 S.M. Picksley, R.G. Lloyd, and C. Buckman 469
RecF and RecBC Function during Recombination of Nonreplicating, UV-irradiated Phage l DNA and during Other Recombination Processes J.B. Hays, T.A. G. Smith, S.A. Friedman, F. Lee, and G.L. Coffman 475
Roles of RecBC Enzyme and Chi Sites in Homologous Recombination G.R. Smith, S.K. Amundsen, A.M. Chaudhury, K.C. Cheng, A.S. Ponticelli, C.M. Roberts, D. W. Schultz, and A.F. Taylor 485
The Mechanism of the Chi-cos Interaction in RecA-RecBC-mediated Recombination in Phage l I. Kobayashi, M.M. Stahl, and F. W. Stahl 497
RecA
A Model for the Core Structure of the Escherichia coli RecA Protein M.A. Blanar, D. Kneller, A.J. Clark, A.E. Karu, F.E. Cohen, R. Lan gridge, and I.D. Kuntz 507
Intermediates in Homologous Pairing Promoted by RecA Protein and Correlations of Recombination In Vitro and In Vivo S.S. Flory, J. Tsang, K. Muniyappa, M. Bianchi, D. Gonda, R. Kahn, F. Azhderian, C. Egner, S. Shaner, and C.M Radding 513
Unidirectional Branch Migration Promoted by Nucleoprotein Filaments of RecA Pro- tein and DNA M.M. Cox, S. W. Morrical, and S.K. Neuendorf 525
Studies of the Mechanism of DNA Pairing by the RecA Protein of Escherichia coli F.R. Bryant, P. W. Riddles, and I.R. Lehman 535
Roles of Processive Unwinding in Recombination Reactions Promoted by RecA Pro- tein of Escherichia coli: A Study Using a Monoclonal Antibody T. Shibata, O. Makino, S. Ikawa, T. Ohtani, M. Iwabuchi, Y. Shibata, H. Maeda, and T. Ando 541
Visualization of SSB-ssDNA Complexes Active in the Assembly of Stable RecA-DNA Filaments J.D. Griffith, L.D. Harris, and J. Register III 553
Visualization of RecA-DNA Complexes Involved in Consecutive Stages of an In Vitro Strand Exchange Reaction A. Stasiak, A. Z. Stasiak, and T. Koller 561
Molecular Mechanisms of General Genetic Recombination: The DNA-binding Sites of the RecA Protein P. Howard-Flanders, S.C. West, J.R. Rusche, and E.H. Egel- man 571
Repair
Studies of HeLa DNA Polymerases during Excision-repair In Vitro with DNA and Chromatin Substrates D. W. Mosbaugh, D.H. Evans, and S. Linn 581
Repair of DNA Base-pair Mismatches in Extracts of Escherichia coli A -L. Lu, K. Welsh, S. Clark, S.-S. Su, and P. Modrich 589
The mutH, mutL, mutS, and uvrD Genes of Salmonella typhimurium LT2 P.P. Pang, S. -D. Tsen, A .S. Lundberg, and G. C. Walker 597
An Escherichia coli Cell-free System That Catalyzes the Repair of Symmetrically Methylated Heteroduplex DNA R.A. Fishel and R. Kolodner Involvement of Escherichia coil Mismatch Repair in DNA Replication and Recombination R. Wagner, C. Dohet, M. Jones, M. -P. Doutriaux, F. Hutch- inson, and M. Radman 611
Formation of Inverted Dimer Plasmids after Transformation of Yeast with Linearized Plasmid DNA S. Kunes, D. Botstein, and M. S. Fox 617
Double-strand-break Repair, Gene Conversion, and Postdivision Segregation R. Roth- stein 629
Eukaryotic Enzymes
Changes in the Chromosomal DNA of Yeast during Meiosis in Repair Mutants and the Possible Role of a Deoxyribonuclease M.A. Resnick, T. Chow, J. Nitiss, and J. Game 639
Integrative Recombination-A Role for the Retroviral Reverse Transcriptase A.M. Skalka, G. Duyk, M. Longiaru, P. DeHaseth, R. Terry, and J. Leis 651
Endonuclease J: A site-specific Endonuclease Cleaving Immunoglobulin Genes S. Kondo, T. Kataoka, M. Nishi, M. Kodaira, S. Takeda, and T. Honjo 661
Biochemical Characterization of recI Mutants and the Genetics Control of Recombi- nation in Ustilago maydis R. Holliday, S. Y. Taylor, E.B. Kmiec, and W.K. Hol- loman 669
Homologous Pairing Promoted by Ustilago Red Protein F. Kmiec, P. Kroeger, R. Holliday, and W. Holloman 675
Poly(ADP-ribosylation) of DNA Topoisomerase I: A Nuclear Response to DNA-strand Interruptions A .M. Ferro, M. C. McElwain, and B.M. Olivera 683
Integration and Excision of Bacteriophage
Primary Structure and the himA Gene of Escherichia coli: Homology with DNA-bind- ing Protein HU and Association with the Phenylalanyl-tRNA Synthetase Operon H.I. Miller 691
DNA Interactions during Bacteriophage l Site-Specific Recombination C.E. Bauer, S.D. Hesse, J.F. Gardner, and R.I. Gumport 699
Site-specific Recombination Systems of Phages f 80 and P22: Binding Sites of Integra- tion Host Factor and Recombination-induced Mutations J. Leong, S. Nunes- Duby, A. Oser, C. Lesser, P. Youderian, M.M. Susskind, and A. Landy 707
Enzymes and Sites of Genetic Recombination: Studies with Gene-3 Endonuclease of Phage T7 and with Site-affinity Mutants of Phage l B. de Massy, F. W. Studier, L. Dorgai, E. Appelbaum, and R.A. Weisberg 715
The Role of Specialized Nucleoprotein Structures in Site-specific Recombination and Initiation of DNA Replication H. Echo Is, M. Dodson, M. Better, J.D. Roberts, and R. McMacken 727
l Integrative Recombination: Supercoiling, Synapsis, and Strand Exchange P. Kitts, E. Richet, and H.A. Nash 735
Quantitative Analysis of the Contributions of Enzyme and DNA to the Structure of l Integrative Recombinants S.J. Spengler, A. Stasiak, A.Z. Stasiak, and N.R. Cozzarelli 745
Site-specific Recombination
In Vitro Analysis of Hin-mediated Site-specific Recombination R. C. Johnson, M.B. Bruist, M.B. Glaccum, and M.L Simon 751
The Nature of the Interaction of the P1 Recombinase Cre with the Recombining Site loxP R. Hoess, K. Abremski, and N. Sternberg 761
The Bacteriophage P1 Site-specific Recombinase Cm: Recombination Events and DNA Recognition Sequences S. Jida, H. Huber, R. Hiestand-Nauer, J. Meyer, T.A. Bickle, and W. Arber 769
Components of the Site-specific Recombination System Encoded by the Yeast Plasmid 2-micron Circle M. McLeod, F. Volkert, and J. Broach 779
In Vitro Systems for Genetic Recombination of the DNAs of Bacteriophage T7 and Yeast 2-micron Circle P.D. Sadowski, D.D. Lee, B.J. Andrews, D. Babineau, L. Beatty, M.J. Morse, G. Proteau, and D. Vetter 789
Site-Specific Genetic Recombination Promoted by the FLP Protein of the Yeast 2- micron Plasmid In Vitro L. Meyer-Leon, J.F. Senecoff, R. C. Bruckner, and M.M. Cox 797
Recombination in Vitro
Genetic Recombination Catalyzed by Cell-free Extracts of Saccharomyces cerevisiae L.S. Symington, P. T. Morrison, and R. Kolodner 805
Resolution of Holliday Structures by Endonuclease VII As Observed in Interactions with Cruciform DNA B. Kemper, F. Jensch, M. v. Depka-Prondzynski, H.-J. Fritz, U. Borgmeyer, and K. Mizuuchi 815
On the Mu Repressor and Early DNA Intermediates of Transposition H.M. Krause and N.P. Higgins 827
The Mechanism of Transposition of Bacteriophage Mu K. Mizuuchi, M. Mizuuchi, and R.. Craigie 835
Summary
Types of Recombination: Common Problems and Common Strategies A. Campbell 839
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