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 XLVI: Organization of the Cytoplasm 1981 |
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From left: Dr. Lewis Tilney, Dr. Thomas Pollard  SYMPOSIUM SYNOPSIS LIST OF PARTICIPANTS CONTENTS OF SYMPOSIUM VOLUME PHOTOGRAPHS PUBLISHED VOLUME  |
Organization of the Cytoplasm
Symposium Volume XLVI Contents
Part 1
Symposium Participants v
Foreword xi
Principles of Organization: Functional and Spatial Order in the Cytoplasm
Membrane Cytoskeletal Interactions in the Human Erythrocyte D. Branton 1
Regulation of Intracellular Protein Traffic G. Blobel 7
Organizing the Cytoplasm for Motility F. Solomon 17
Principles of Organization: Water and the Cytoplasmic Architecture
Interrelationships between Water and Cell Metabolism in Artemia Cysts. IX. Evidence for Organization of Soluble Cytoplasmic Enzymes J. S. Clegg 23
Measurements of the Diffusion of Macromolecules Injected into the Cytoplasm of Living Cells J. W. Wojcieszyn, R. A. Schiegel, and K. A. Jacobson 39
Does Blebbing Reveal the Convulsive Flow of Liquid and Solutes through the Cy- toplasmic Meshwork? G. Albrecht-Buehler 45
Structural Organization of the Cytoplasm M. Schliwa, J. van Blerkom, and K. B. Pryzwansky 51
Cytoplasmic and Axonal Flow
Active Movement of Bundles of Actin and Myosin Filaments from Muscle: A Simple Model for Cell Motility S. Higashi-Fujime 69
Contraction-Relaxation Cycle of Physarum Cytoplasm: Concomitant Changes in In- traplasmodial ATP and Ca++ Concentrations N. Kamiya, Y. Yoshimoto, and F. Matsumura 77
Cytoplasmic Transport: Moving Ultrastructural Elements Common to Many Cell Types Revealed by Video-enhanced Microscopy R. D. Allen, J. L. Travis, J. H. Hay- den, N. S. Allen, A. C. Breuer, and L. J. Lewis 85 Fine Structure and Organization of Microfilaments in Physarum as Revealed by Chemolixation and Freeze-etching K. E. Wohlfarth-Bottermann and W. Stockem 89
Cellular and Molecular Aspects of Amoeboid Movement D. L. Taylor, J. Heiple, Y.-L. Wang, E. J. Luna, L. Tanasugarn, J. Brier, J. Swanson, M. Fechheimer, P. Amato, M. Rockwell, and G. Daley 101
The Axon: A Prototype for Studying Expressional Cytoplasm R. J. Lasek and S. T. Brady 113
Communication between Target Organs and Nerve Cells: Retrograde Axonal Transport and Site of Action of Nerve Growth Factor M. E. Schwab, R. Heumann, and H. Thoenen 125
Studies on the Mechanism of Fast Axoplasmic Transport Using Microinjection into Single Giant Neurons D. J. Goldberg, D. A. Harris, and J. H. Schwartz 135
Probing Mitochondria in Living Cells with Rhodamine 123 L. B. Chen, I. C. Sum- merhayes, L. V. Johnson, M. L. Walsh, S. D. Bernal, and T. J. Lampidis 141
Elements of Organization: Microtubules
Expression of Flagellar Protein Genes during Flagellar Regeneration in Chlamydo- monas C. D. Silfiow, P. A. Lefebvre, T. W. McKeithan, J. A. Schloss, L. R. Keller, and J. L. Rosenbaum 157
Autoregulatory Control of the Expression of a- and b-Tubulins: Implications for Mi- crotubule Assembly D. Cleveland and M. Kirschner 171
Drosophila a-Tubulin Genes and Their Transcription Patterns L. Kalfayan, J. Loew- enberg, and P. C. Wensink 185
Amino Acid Sequence of a- and b-Tubulins from Pig Brain: Heterogeneity and Regional Similarity to Muscle Proteins H. Ponstingl, E. Krauhs, M. Little, T. Kempf, R. Hofer-Warbinek, and W. Ade 191
Microtubule Treadmills and Their Possible Cellular Functions L. Wilson and R. L. Margolis 199
Dynein-Microtubule Interactions: ATP-sensitive Dynein Binding and the Structural Polarity of Mitotic Microtubules L. T. Haimo and B. R. Telzer 207
Taxol: A New Probe for Studying the Structure and Function of Microtubules S. B. Horwitz, J. Parness, P. B. Schiff and J. J. Manfredi 219
Microtubule Stability and Assembly in Living Cells: The Influence of Metabolic In- hibitors, Taxol and pH M. De Brabander, G. Geuens, R. Nuydens, R. Wille- brords, and J. De Mey 227
Tubulin Assembly Sites and the Organization of Microtubule Arrays in Mammalian Cells S. L. Brenner and B. R. Brinkley 241
Microtubule Structure in Caenorhabditis elegans Neurons M. Chalfie 255
Asters, Poles, and Transport Properties within Spindlelike Microtubule Arrays A. S. Bajer and J. Molè-Bajer 263
Approaches to Potential Sliding Mechanisms of Cytoplasmic Microtubules P. Satir 285
Elements of Organization: Intermediate Filaments
Differentiated Structural Components of the Keratinocyte H. Green, E. Fuchs, and F. Watt 293
Immunolabeling Studies of Cytoskeletal Associations in Cultured Cells S. J. Singer, E. H. Ball, B. Geiger, and W.-T. Chen 303
Intermediate-size Filaments: Changes in Synthesis and Distribution in Cells of the Myogenic and Neurogenic Lineages H. Holtzer, G. S. Bennett, S. J. Tapscott, J. M. Croop, and Y. Toyama 317
Use of Antibodies to Intermediate Filaments in the Characterization of Human Tu- mors F. C. S. Ramaekers, J. J. G. Puts, A. Kant, O. Moesker, P. H. K. Jap, and G. P. Vooijs 331
Chemistry and Biology of Neuronal and Glial Intermediate Filaments R. K. H. Liem, C. H. Keith, J. F. Leterrier, E. Trenkner, and M. L. Shelanski 341 Desmin- and Vimentin-containing Filaments and Their Role in the Assembly of the Z Disk in Muscle Cells E. Lazarides, B. L. Granger, D. L. Gard, C. M. O'Connor, J. Breckler, M. Price, and 5. I. Danto 351
Modification of Vimentin Polypeptides during Mitosis R. Bravo, S. J. Fey, P. Mose Larsen, and J. E. Celis 379
Epithelial Tonofilaments: Investigating Their Form and Function Using Monoclonal Antibodies E. B. Lane and M. W. Klymkowsky 387
In Vivo Disruption of the Cytokeratin Cytoskeleton in Cultured Epithelial Cells by Microinjection of Antikeratin: Evidence for the Presence of an Intermediate- filament-organizing Center B. S. Eckert, R. A. Daley, and L. M. Parysek 403
Intermediate Filaments M. Osborn, N. Geisler, G. Shaw, G. Sharp, and K. Weber 413
Differentiation-related Patterns of Expression of Proteins of Intermediate-size Fila- ments in Tissues and Cultured Cells W. W. Franke, E. Schmid, D. L. Schiller, S. Winter, E. D. Jarasch, R. Moll, H. Denk, B. W. Jackson, and K. Illmensee 431
Structure of Vimentin 10-nm Filaments Probed with a Monoclonal Antibody That Recognizes a Common Antigenic Determinant on Vimentin and Tropo- myosin S. H. Blose, F. Matsumura, and J. J.-C. Liv 455
Heterogeneity of Intermediate Filaments Assembled In Vitro P. Steinert, W. Idler, M. Aynardi-Whiiman, R. Zackroff, and R. D. Goldman 465
Plectin: A High-molecular-weight Cytoskeletal Polypeptide Component That Copu- rifles with Intermediate Filaments of the Vimentin Type C. Wiche, H. Herrmann, F. Leichtfried, and R. Pytela 475
Formation of an ATP-dependent Microtubule-Neurofilament Complex In Vitro M. S. Runge and R. C. Williams, Jr 483
Part 2
Elements of Organization: Microfilaments
Actin Multigene Family of Dictyostelium M. McKeown and R. A. Firtel 495
Approaches for Isolating Genes Encoding the Cytoskeletal Proteins and for Reintro- ducing the Genes into Cultured Cells S. H. Hughes, J. Sorge, G. P. Thomas, J. Feramisco, L. Chow, and J. Garrels 507
Actin Structure, Polymerization, and Gelation T. D. Pollard, U. Aebi, J. A. Cooper, W. E. Fowler, and P. Tseng 513
How Actin Filaments Pack into Bundles D. J. DeRosier and L. G. Tilney 525
Calcium-modulated Multifunctional Proteins Regulating F-Actin Organization K. Weber and J. R. Glenney, Jr 541
Control of Assembly of Dictyostelium Myosin and Actin Filaments J. A. Spudich, E. R. Kuczmarski, J. D. Pardee, P. A. Simpson, K. Yamamoto, and L. Stryer 553
Function of Cytoplasmic Flow in Photosensory Transduction and Phase Regulation of Contractile Activities in Physarum K. E. Wohlfarth-Bottermann and I. Block 563
Actin Gelation and the Structure of Cortical Cytoplasm T. P. Stossel, J. H. Hartwig, H. L. Yin, K. S. Zaner, and O. I. Stendahl 569
Actinogelin: A Ca4 + -sensitive Regulatory Protein of Microfilament Organization N. Mimura and A. Asano 579
a-Actinin and Vinculin from Nonmuscle Cells: Calcium-sensitive Interactions with Actin K. Burridge and J. R. Feramisco 587
Organization of Actin Meshworks in Cultured Cells: The Leading Edge J. V. Small, G. Rinnerthaler, and H. Hinssen 599
Vinculin and a-Actinin: Interaction with Actin and Effect on Microfilament Network Formation B. M. Jockusch and G. Isenberg 613
Proteins and Complexes That Affect Actin-filament Assembly and Interactions S. Lin, J. A. Wilkins, D. H. Cribbs, M. Grumet, and D. C. Lin 625
Cell Surface: Surface Organization
Partial Sequence and Turnover of Rat Liver Gap Junction Protein J.-P. Revel, B. J. Nicholson, and S. B. Yancey 633
Liver Gap Junctions and Lens Fiber Junctions: Comparative Analysis and Calmodulin Interaction E. L. Hertzberg and N. B. Gilula 639
Immunoreactive Forms of Human Erythrocyte Ankyrin Are Localized in Mitotic Structures in Cultured Cells and Are Associated with Microtubules in Brain V. Bennett and J. Davis 647
Relationships between Microfilaments, Cell-substratum Adhesion, and Fibronec- tin R. O. Hynes, A. T. Destree, and D. D. Wagner 659
Involvement of Vinculin in Contact-induced Cytoskeletal Interactions B. Geiger 671
Flagellum as a Model System for Studying Dynamic Cell-surface Events R. A. Blood- good 683
Directed Membrane Flow on the Pseudopods of Caenorhabditis elegans Spermato- zoa T. M. Roberts and S. Ward 695
Packing of Clathrin into Coats B. M. F. Pearse and R. A. Crowther 703
Surface Uptake by Fibroblasts and Its Consequences M. S. Bretscher 707
Recycling of Cell-surface Receptors: Observations from the LDL Receptor Sys- tem M. S. Brown, R. G. W. Anderson, S. K. Basu, and J. L. Goldstein 713
Functional and Structural Studies on Clathrin Triskelions and Baskets E. Unge- wickell, E. R. Unanue, and D. Branton 723
Receptor-mediated Endocytosis by Clathrin-coated Vesicles: Evidence for a Dynamic Pathway J. L. Salisbury, J. S. Condeelis, N. J. Maihle, and P. Satir 733
Microinjection of Anticlathrin Antibodies into Cultured Fibroblasts: Clathrin-coated Structures in Receptor-mediated Endocytosis and in Exocytosis J. Wehland, M. C. Willingham, M. G. Gallo, A. V. Rutherford, J. Rudick, R. B. Dickson, and I. Pastan 743
Intracellular and Extracellular Components Involved in the Formation of Ventral Surfaces of Fibroblasts W. Birchmeier, T. A. Libermann, B. A. Imhof, and T. E. Kreis 755
Cell Surface: Synthesis, Exocytosis, Endocytosis
Mapping Structural Proteins of Cultured Cells by Monoclonal Antibodies J. J.-C. Lin 769
Synthesis and Assembly of the Vesicular Stomatitis Virus Glycoprotein A. Zilber- stein, M. D. Snider, and H. F. Lodish 785
The Golgi Apparatus, Coated Vesicles, and the Sorting Problem J. E. Rothman, E. Fries, W. G. Dunphy, and L. J. Urbani 797
Mechanisms for the Incorporation of Proteins into the Plasma Membrane D. Sabatini, D. Colman, E. Sabban, J. Sherman, T. Morimoto, G. Kreibich, and M. Adesnik 807
Calcium-dependent Regulation of Chromaffin Granule Movement, Membrane Contact, and Fusion during Exocytosis H. B. Pollard, C. E. Creutz, V. Fowler, J. Scott, and C. J. Pazoles 819
Are Lysosomes a Site of Enveloped-virus Penetration? M. Marsh, K. Matlin, K. Simons, H. Reggio, J. White, J. Kartenbeck, and A. Helenius 835
Cell Surface: Microvilli
Structure and Function of the Brush-border Cytoskeleton P. T. Matsudaira and D. R. Burgess 845
Regulation of Contractility, Cytoskeletal Structure, and Filament Assembly in the Brush Border of Intestinal Epithelial Cells M. S. Mooseker, E. M. Bonder, B. G. Grimwade, C. L. Howe, T. C. S. Keller III, R. H. Wasserman, and K. A. Wharton 855
Characterization and Ultrastructural Role of the Major Components of the Intestinal Microvillus Cytoskeleton A. Bretscher 871
The Cytoskeleton of Intestinal Microvilli Contains Two Polypeptides Immunologically Related to Proteins of Striated Muscle E. Coudrier, H. Reggio, and D. Louvard 881
Mechanisms of Organization: Calcium Regulation and Phosphorylation of Cytoplasmic Components
Multifunctional Role of Calmodulin in Biologic Processes R. W. Wallace, E. A. Tallant, and W. Y. Cheung 893
Regulation by and of Calmodulin in Mammalian Cells A. R. Means and J. G. Chaf- ouleas 903
Molecular Events in cAMP-mediated Reverse Transformation A. H. Lockwood, D. D. Trivette, and M. Pendergast 909
Regulation of Actin-Myosin Interaction by Reversible Phosphorylation of Myosin and Myosin Kinase R. S. Adeistein, M. D. Pato, J. R. Sellers, P. de Lanerolle, and M. A. Conti 921
Regulation of Myosin-filament Assembly by Light-chain Phosphorylation J. Ken- drick-Jones, P. Tooth, K. A. Taylor, and J. M. Scholey 929
Cytoskeletal Targets for Viral Transforming Proteins with Tyrosine Protein Kinase Activity B. M. Sefton, T. Hunter, E. A. Nigg, S. J. Singer, and G. Walter 939
Mechanism of Transformation by Rous Sarcoma Virus: Events within Adhesion Plaques L. Rohrschneider, M. Rosok, and K. Shrive 953
Nucleus and Cytoplasm
Nuclear Lamina and the Structural Organization of the Nuclear Envelope L. Gerace and G. Blobel 967
Synthesis and Assembly of the Polypeptide Subunits of Photosystem I J. E. Mullet, A. R. Grossman, and H.-H. Chua 979
Molecular and Cellular Effects of Heat-shock and Related Treatments of Mammalian Tissue-culture Cells G. P. Thomas, W. J. Welch, M. B. Mathews, and J. R. Feramisco 985
Function of Cytoplasmic Fibers in Syncytia E. Wang, D. S. Roos, M. H. Heggeness, and P. W. Choppin 997
Cytoplasmic and Nuclear Architecture in Cells and Tissue: Form, Functions, and Mode of Assembly S. Penman, A. Fulton, D. Capco, A. Ben Ze'ev, S. Wit- telsburger, and C. F. Tse 1013
Summary Organization of the Cytoplasm B. R. Brinkley 1029
Symposium Participants v
Foreword xi
Principles of Organization: Functional and Spatial Order in the Cytoplasm
Membrane Cytoskeletal Interactions in the Human Erythrocyte D. Branton 1
Regulation of Intracellular Protein Traffic G. Blobel 7
Organizing the Cytoplasm for Motility F. Solomon 17
Principles of Organization: Water and the Cytoplasmic Architecture
Interrelationships between Water and Cell Metabolism in Artemia Cysts. IX. Evidence for Organization of Soluble Cytoplasmic Enzymes J. S. Clegg 23
Measurements of the Diffusion of Macromolecules Injected into the Cytoplasm of Living Cells J. W. Wojcieszyn, R. A. Schiegel, and K. A. Jacobson 39
Does Blebbing Reveal the Convulsive Flow of Liquid and Solutes through the Cy- toplasmic Meshwork? G. Albrecht-Buehler 45
Structural Organization of the Cytoplasm M. Schliwa, J. van Blerkom, and K. B. Pryzwansky 51
Cytoplasmic and Axonal Flow
Active Movement of Bundles of Actin and Myosin Filaments from Muscle: A Simple Model for Cell Motility S. Higashi-Fujime 69
Contraction-Relaxation Cycle of Physarum Cytoplasm: Concomitant Changes in In- traplasmodial ATP and Ca++ Concentrations N. Kamiya, Y. Yoshimoto, and F. Matsumura 77
Cytoplasmic Transport: Moving Ultrastructural Elements Common to Many Cell Types Revealed by Video-enhanced Microscopy R. D. Allen, J. L. Travis, J. H. Hay- den, N. S. Allen, A. C. Breuer, and L. J. Lewis 85 Fine Structure and Organization of Microfilaments in Physarum as Revealed by Chemolixation and Freeze-etching K. E. Wohlfarth-Bottermann and W. Stockem 89
Cellular and Molecular Aspects of Amoeboid Movement D. L. Taylor, J. Heiple, Y.-L. Wang, E. J. Luna, L. Tanasugarn, J. Brier, J. Swanson, M. Fechheimer, P. Amato, M. Rockwell, and G. Daley 101
The Axon: A Prototype for Studying Expressional Cytoplasm R. J. Lasek and S. T. Brady 113
Communication between Target Organs and Nerve Cells: Retrograde Axonal Transport and Site of Action of Nerve Growth Factor M. E. Schwab, R. Heumann, and H. Thoenen 125
Studies on the Mechanism of Fast Axoplasmic Transport Using Microinjection into Single Giant Neurons D. J. Goldberg, D. A. Harris, and J. H. Schwartz 135
Probing Mitochondria in Living Cells with Rhodamine 123 L. B. Chen, I. C. Sum- merhayes, L. V. Johnson, M. L. Walsh, S. D. Bernal, and T. J. Lampidis 141
Elements of Organization: Microtubules
Expression of Flagellar Protein Genes during Flagellar Regeneration in Chlamydo- monas C. D. Silfiow, P. A. Lefebvre, T. W. McKeithan, J. A. Schloss, L. R. Keller, and J. L. Rosenbaum 157
Autoregulatory Control of the Expression of a- and b-Tubulins: Implications for Mi- crotubule Assembly D. Cleveland and M. Kirschner 171
Drosophila a-Tubulin Genes and Their Transcription Patterns L. Kalfayan, J. Loew- enberg, and P. C. Wensink 185
Amino Acid Sequence of a- and b-Tubulins from Pig Brain: Heterogeneity and Regional Similarity to Muscle Proteins H. Ponstingl, E. Krauhs, M. Little, T. Kempf, R. Hofer-Warbinek, and W. Ade 191
Microtubule Treadmills and Their Possible Cellular Functions L. Wilson and R. L. Margolis 199
Dynein-Microtubule Interactions: ATP-sensitive Dynein Binding and the Structural Polarity of Mitotic Microtubules L. T. Haimo and B. R. Telzer 207
Taxol: A New Probe for Studying the Structure and Function of Microtubules S. B. Horwitz, J. Parness, P. B. Schiff and J. J. Manfredi 219
Microtubule Stability and Assembly in Living Cells: The Influence of Metabolic In- hibitors, Taxol and pH M. De Brabander, G. Geuens, R. Nuydens, R. Wille- brords, and J. De Mey 227
Tubulin Assembly Sites and the Organization of Microtubule Arrays in Mammalian Cells S. L. Brenner and B. R. Brinkley 241
Microtubule Structure in Caenorhabditis elegans Neurons M. Chalfie 255
Asters, Poles, and Transport Properties within Spindlelike Microtubule Arrays A. S. Bajer and J. Molè-Bajer 263
Approaches to Potential Sliding Mechanisms of Cytoplasmic Microtubules P. Satir 285
Elements of Organization: Intermediate Filaments
Differentiated Structural Components of the Keratinocyte H. Green, E. Fuchs, and F. Watt 293
Immunolabeling Studies of Cytoskeletal Associations in Cultured Cells S. J. Singer, E. H. Ball, B. Geiger, and W.-T. Chen 303
Intermediate-size Filaments: Changes in Synthesis and Distribution in Cells of the Myogenic and Neurogenic Lineages H. Holtzer, G. S. Bennett, S. J. Tapscott, J. M. Croop, and Y. Toyama 317
Use of Antibodies to Intermediate Filaments in the Characterization of Human Tu- mors F. C. S. Ramaekers, J. J. G. Puts, A. Kant, O. Moesker, P. H. K. Jap, and G. P. Vooijs 331
Chemistry and Biology of Neuronal and Glial Intermediate Filaments R. K. H. Liem, C. H. Keith, J. F. Leterrier, E. Trenkner, and M. L. Shelanski 341 Desmin- and Vimentin-containing Filaments and Their Role in the Assembly of the Z Disk in Muscle Cells E. Lazarides, B. L. Granger, D. L. Gard, C. M. O'Connor, J. Breckler, M. Price, and 5. I. Danto 351
Modification of Vimentin Polypeptides during Mitosis R. Bravo, S. J. Fey, P. Mose Larsen, and J. E. Celis 379
Epithelial Tonofilaments: Investigating Their Form and Function Using Monoclonal Antibodies E. B. Lane and M. W. Klymkowsky 387
In Vivo Disruption of the Cytokeratin Cytoskeleton in Cultured Epithelial Cells by Microinjection of Antikeratin: Evidence for the Presence of an Intermediate- filament-organizing Center B. S. Eckert, R. A. Daley, and L. M. Parysek 403
Intermediate Filaments M. Osborn, N. Geisler, G. Shaw, G. Sharp, and K. Weber 413
Differentiation-related Patterns of Expression of Proteins of Intermediate-size Fila- ments in Tissues and Cultured Cells W. W. Franke, E. Schmid, D. L. Schiller, S. Winter, E. D. Jarasch, R. Moll, H. Denk, B. W. Jackson, and K. Illmensee 431
Structure of Vimentin 10-nm Filaments Probed with a Monoclonal Antibody That Recognizes a Common Antigenic Determinant on Vimentin and Tropo- myosin S. H. Blose, F. Matsumura, and J. J.-C. Liv 455
Heterogeneity of Intermediate Filaments Assembled In Vitro P. Steinert, W. Idler, M. Aynardi-Whiiman, R. Zackroff, and R. D. Goldman 465
Plectin: A High-molecular-weight Cytoskeletal Polypeptide Component That Copu- rifles with Intermediate Filaments of the Vimentin Type C. Wiche, H. Herrmann, F. Leichtfried, and R. Pytela 475
Formation of an ATP-dependent Microtubule-Neurofilament Complex In Vitro M. S. Runge and R. C. Williams, Jr 483
Part 2
Elements of Organization: Microfilaments
Actin Multigene Family of Dictyostelium M. McKeown and R. A. Firtel 495
Approaches for Isolating Genes Encoding the Cytoskeletal Proteins and for Reintro- ducing the Genes into Cultured Cells S. H. Hughes, J. Sorge, G. P. Thomas, J. Feramisco, L. Chow, and J. Garrels 507
Actin Structure, Polymerization, and Gelation T. D. Pollard, U. Aebi, J. A. Cooper, W. E. Fowler, and P. Tseng 513
How Actin Filaments Pack into Bundles D. J. DeRosier and L. G. Tilney 525
Calcium-modulated Multifunctional Proteins Regulating F-Actin Organization K. Weber and J. R. Glenney, Jr 541
Control of Assembly of Dictyostelium Myosin and Actin Filaments J. A. Spudich, E. R. Kuczmarski, J. D. Pardee, P. A. Simpson, K. Yamamoto, and L. Stryer 553
Function of Cytoplasmic Flow in Photosensory Transduction and Phase Regulation of Contractile Activities in Physarum K. E. Wohlfarth-Bottermann and I. Block 563
Actin Gelation and the Structure of Cortical Cytoplasm T. P. Stossel, J. H. Hartwig, H. L. Yin, K. S. Zaner, and O. I. Stendahl 569
Actinogelin: A Ca4 + -sensitive Regulatory Protein of Microfilament Organization N. Mimura and A. Asano 579
a-Actinin and Vinculin from Nonmuscle Cells: Calcium-sensitive Interactions with Actin K. Burridge and J. R. Feramisco 587
Organization of Actin Meshworks in Cultured Cells: The Leading Edge J. V. Small, G. Rinnerthaler, and H. Hinssen 599
Vinculin and a-Actinin: Interaction with Actin and Effect on Microfilament Network Formation B. M. Jockusch and G. Isenberg 613
Proteins and Complexes That Affect Actin-filament Assembly and Interactions S. Lin, J. A. Wilkins, D. H. Cribbs, M. Grumet, and D. C. Lin 625
Cell Surface: Surface Organization
Partial Sequence and Turnover of Rat Liver Gap Junction Protein J.-P. Revel, B. J. Nicholson, and S. B. Yancey 633
Liver Gap Junctions and Lens Fiber Junctions: Comparative Analysis and Calmodulin Interaction E. L. Hertzberg and N. B. Gilula 639
Immunoreactive Forms of Human Erythrocyte Ankyrin Are Localized in Mitotic Structures in Cultured Cells and Are Associated with Microtubules in Brain V. Bennett and J. Davis 647
Relationships between Microfilaments, Cell-substratum Adhesion, and Fibronec- tin R. O. Hynes, A. T. Destree, and D. D. Wagner 659
Involvement of Vinculin in Contact-induced Cytoskeletal Interactions B. Geiger 671
Flagellum as a Model System for Studying Dynamic Cell-surface Events R. A. Blood- good 683
Directed Membrane Flow on the Pseudopods of Caenorhabditis elegans Spermato- zoa T. M. Roberts and S. Ward 695
Packing of Clathrin into Coats B. M. F. Pearse and R. A. Crowther 703
Surface Uptake by Fibroblasts and Its Consequences M. S. Bretscher 707
Recycling of Cell-surface Receptors: Observations from the LDL Receptor Sys- tem M. S. Brown, R. G. W. Anderson, S. K. Basu, and J. L. Goldstein 713
Functional and Structural Studies on Clathrin Triskelions and Baskets E. Unge- wickell, E. R. Unanue, and D. Branton 723
Receptor-mediated Endocytosis by Clathrin-coated Vesicles: Evidence for a Dynamic Pathway J. L. Salisbury, J. S. Condeelis, N. J. Maihle, and P. Satir 733
Microinjection of Anticlathrin Antibodies into Cultured Fibroblasts: Clathrin-coated Structures in Receptor-mediated Endocytosis and in Exocytosis J. Wehland, M. C. Willingham, M. G. Gallo, A. V. Rutherford, J. Rudick, R. B. Dickson, and I. Pastan 743
Intracellular and Extracellular Components Involved in the Formation of Ventral Surfaces of Fibroblasts W. Birchmeier, T. A. Libermann, B. A. Imhof, and T. E. Kreis 755
Cell Surface: Synthesis, Exocytosis, Endocytosis
Mapping Structural Proteins of Cultured Cells by Monoclonal Antibodies J. J.-C. Lin 769
Synthesis and Assembly of the Vesicular Stomatitis Virus Glycoprotein A. Zilber- stein, M. D. Snider, and H. F. Lodish 785
The Golgi Apparatus, Coated Vesicles, and the Sorting Problem J. E. Rothman, E. Fries, W. G. Dunphy, and L. J. Urbani 797
Mechanisms for the Incorporation of Proteins into the Plasma Membrane D. Sabatini, D. Colman, E. Sabban, J. Sherman, T. Morimoto, G. Kreibich, and M. Adesnik 807
Calcium-dependent Regulation of Chromaffin Granule Movement, Membrane Contact, and Fusion during Exocytosis H. B. Pollard, C. E. Creutz, V. Fowler, J. Scott, and C. J. Pazoles 819
Are Lysosomes a Site of Enveloped-virus Penetration? M. Marsh, K. Matlin, K. Simons, H. Reggio, J. White, J. Kartenbeck, and A. Helenius 835
Cell Surface: Microvilli
Structure and Function of the Brush-border Cytoskeleton P. T. Matsudaira and D. R. Burgess 845
Regulation of Contractility, Cytoskeletal Structure, and Filament Assembly in the Brush Border of Intestinal Epithelial Cells M. S. Mooseker, E. M. Bonder, B. G. Grimwade, C. L. Howe, T. C. S. Keller III, R. H. Wasserman, and K. A. Wharton 855
Characterization and Ultrastructural Role of the Major Components of the Intestinal Microvillus Cytoskeleton A. Bretscher 871
The Cytoskeleton of Intestinal Microvilli Contains Two Polypeptides Immunologically Related to Proteins of Striated Muscle E. Coudrier, H. Reggio, and D. Louvard 881
Mechanisms of Organization: Calcium Regulation and Phosphorylation of Cytoplasmic Components
Multifunctional Role of Calmodulin in Biologic Processes R. W. Wallace, E. A. Tallant, and W. Y. Cheung 893
Regulation by and of Calmodulin in Mammalian Cells A. R. Means and J. G. Chaf- ouleas 903
Molecular Events in cAMP-mediated Reverse Transformation A. H. Lockwood, D. D. Trivette, and M. Pendergast 909
Regulation of Actin-Myosin Interaction by Reversible Phosphorylation of Myosin and Myosin Kinase R. S. Adeistein, M. D. Pato, J. R. Sellers, P. de Lanerolle, and M. A. Conti 921
Regulation of Myosin-filament Assembly by Light-chain Phosphorylation J. Ken- drick-Jones, P. Tooth, K. A. Taylor, and J. M. Scholey 929
Cytoskeletal Targets for Viral Transforming Proteins with Tyrosine Protein Kinase Activity B. M. Sefton, T. Hunter, E. A. Nigg, S. J. Singer, and G. Walter 939
Mechanism of Transformation by Rous Sarcoma Virus: Events within Adhesion Plaques L. Rohrschneider, M. Rosok, and K. Shrive 953
Nucleus and Cytoplasm
Nuclear Lamina and the Structural Organization of the Nuclear Envelope L. Gerace and G. Blobel 967
Synthesis and Assembly of the Polypeptide Subunits of Photosystem I J. E. Mullet, A. R. Grossman, and H.-H. Chua 979
Molecular and Cellular Effects of Heat-shock and Related Treatments of Mammalian Tissue-culture Cells G. P. Thomas, W. J. Welch, M. B. Mathews, and J. R. Feramisco 985
Function of Cytoplasmic Fibers in Syncytia E. Wang, D. S. Roos, M. H. Heggeness, and P. W. Choppin 997
Cytoplasmic and Nuclear Architecture in Cells and Tissue: Form, Functions, and Mode of Assembly S. Penman, A. Fulton, D. Capco, A. Ben Ze'ev, S. Wit- telsburger, and C. F. Tse 1013
Summary Organization of the Cytoplasm B. R. Brinkley 1029
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