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 XLIV: Viral Oncogenes 1979 |
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Symposia Dinner, 1979  SYMPOSIUM SYNOPSIS LIST OF PARTICIPANTS CONTENTS OF SYMPOSIUM VOLUME PHOTOGRAPHS PUBLISHED VOLUME  |
Viral Oncogenes
Symposium Volume XLIV Contents
PART 1
DNA TUMOR VIRUSES
Symposium Participants v
Foreword xvii
Viral Oncogenes H. M. Temin 1
Synopses
Transforming Genes of DNA Tumor Viruses L. V. Crawford 9
Transforming Genes of Retroviruses P. H. Duesberg 13
DNA Tumor Viruses: Early RNAs and Early Proteins
Transcription of the SV40 Genome in Virus-transformed Cells and Early Lytic Infection P. K. Ghosh, M. Piatak, V. B. Reddy, J. Swinscoe, P. Lebowitz, and S. M. Weissman 31
Regulation of 5V40 Gene Expression through RNA Splicing G. Khoury, J. C. Aiwine, R. Dhar, P. Gruss, C.-J. Lai, S. Segal, and L Seif 41
A Third Splice Site in SV40 Early mRNA D. F. Mark and P. Berg 55
Comparison of Polyoma Virus Transcription in Productively Infected Mouse Cells and Transformed Rodent Cell Lines R. Kamen, J. Favaloro, J. Parker, R. Treisman, L. Lania, M. Fried, and A. Mellor 63
Transcription of SV40 DNA in Transformed Rat Cells M. Grigoryan, P. Chumakov, and E. Lukanidin 77
Expression of Transforming Viral Genes in Semipermissive Cells Transformed by SV40 or Adenovirus Type 2 or Type 5 S. J. Flint and G. A. Beltz 89
Catalytic Properties of the SV40 Large T Antigen R. Tjian, A. Robbins, and R. Clark 103
Enzymatic Activities Associated with the SV40 Large T Antigen J. D. Griffin, G. Spangler, and D. M. Livingston 113
DNA Binding and Sedimentation Properties of SV40 T Antigens Synthesized In Vivo and In Vitro C. Prives, Y. Beck, D. Gidoni, M. Oren, and H. Shure 123
Organization of T Antigens in the Polyoma Virus Genome T. Hunter, M. A. Hutchinson, W. Eckhart, T. Friedmann, A. Esty, P. La Porte, and P. Deininger 131
Is Polyoma Virus Middle T Antigen a Protein Kinase? A. E. Smith, M. Fried, Y. Ito, N. Spurr, and R. Smith 141
T Antigens
Polyoma Virus T Antigens Expressed in Transformed Cells: Significance of Middle T Antigen in Transformation Y. Ito and N. Spurr 149
Purification and Characterization of the SV40 F-gene Protein P. Tegtmeyer, T. Spillman, and F. R. Schuetz 159
Phosphorylation of SV40 Large T Antigen G. Walter and P. J. Flory, Jr 165
T Antigens of SV40-transformed Cells F. McCormick, F. Chaudry, R. Harvey, R. Smith, P. W. J. Rigby, E. Paucha, and A. F. Smith 171
Characterization of the Complex between SV40 Large T Antigen and the 53K Host Protein in Transformed Mouse Cells L. V. Crawford, D. P. Lane, D. T. Denhardt, E. F. Harlow, P. M. Nicklin, K. Osborn, and D. C. Pim 179
New Genetic Information Expressed in SV40-transformed Cells: Characterization of the 55K Proteins and Evidence for Unusual SV40 mRNAs P. May, M. Kress, M. Lange, and F. May 189
T-antigen-associated Proteins Induced by SV40 Transformation J. A. Melero, D. S. Greenspan, and R. B. Carroll 201
SV40 17K Protein Is Associated with Two Cellular Proteins K. Rundell, P. Hearing, and Y. C. Yang 211
Characterization of a Murine Cellular SV40 Antigen in SV40-transformed Cells and in Uninfected Embryonal Carcinoma Cells D. I. H. Linzer, W. Maltzman, and A. J. Levine 215
SV40 T-antigen-related Molecules on the Surfaces of HeLa Cells Infected with Adenovirus-2-SV40 Hybrids and on SV40-transformed Cells W. Deppert and R. Henning 225
SV40 Transplantation Antigen: Relationship to SV40-specific Proteins S. S. Tevethia, R. S. Greenfield, D. C. Flyer, and M. J. Tevethia 235
Host Antigens in the Centriolar Region Are Induced in SV40-infected TC7 Cells: SV40 Small-T-function Requirement H. Kasamatsu, M. Shyamala, and W. Lin 243
Transformation Induced by Epstein-Barr Virus and the Role of the Nuclear Antigen G. Klein, J. Luka, and J. Zeuthen 253
The Genetics of Transformation by DNA Tumor Viruses
The hr-t Gene of Polyoma Virus T. L. Benjamin, G. G. Carmichael, and B. S. Schaffhausen 263
Early Mutants of Polyoma Virus (dl8 and dl23) with Altered Transformation Proper- ties: Is Polyoma Virus Middle T Antigen a Transforming Gene Product? B. E. Griffin, Y. Ito, U. Novak, N. Spurr, S. Dilworth, N. Smolar, R. Pollack, K. Smith, and D. Rifkin 271
Deletion Mutants of SV4O That Affect the Structure of Viral Tumor Antigens J. M. Pipas, S. P. Adler, K. W. C. Peden, and D. Nathans 285
Origin-defective Mutants of SV40 Y. Gluzman, R. J. Frisque, and 1. Sambrook 293
Lytic Infection of Teratocarcinoma Cells by Polyoma Virus Mutants M. Vasseur, C. Kress, N. Montreau, and D. Blangy 301
SV40 Mutant tsA 1499 Is Heat-sensitive for Lytic Growth but Generates Cold-sensitive Rat-cell Transformants D. Pintel, N. Bouck, G. di Mayorca, B. Thimmappaya, B. Swerdlow, and T. Shenk 305
Roles of the T Antigens in Transformation by SV40 R. G. Martin, V. P. Setlow, A. B. Chepelinsky, R. Seif, A. M. Lewis, Jr., C. D. Scher, C. D. Stiles, and J. Avila 311
Requirement for the Large T and Small T Proteins of 5V40 in the Maintenance of the Transformed State R. J. Frisque, D. B. Rifkin, and W. C. Topp 325
Host-virus Interactions Critical for Cellular Transformation by Polyoma Virus and SV40 M. Rassoulzadegan, E. Mougneau, B. Perbal, P. Gaudray, F. Birg, and F. Cuzin 333
Viral and Cellular Control of the SV40-transformed Phenotype J. B. Hiscott and V. Defendi 343
Location of Additional Early Regions within the Left Quarter of the Adenoviral Genome J. Williams, R. S. Galos, M. H. Binger, and S. J. Flint 353
Functional Analysis of Adenovirus-5 Host-range Deletion Mutants Defective for Transformation of Rat Embryo Cells T. Shenk, N. Jones, W. Colby, and D. Fowlkes 367
SV-induced Somatic Mutations: Possible Relevance to Viral Transformation M. Theile, M. Strauss, L. Luebbe, S. Scherneck, H. Krause, and F. Geissler 377
The Transforming Genes of Adenoviruses and Their Expression
Structure and Function of the Transforming Genes of Human Adenoviruses and SV40 AJ. van der Eb, H. van Ormondt, P. I. Schrier, J. H. Lupker, H. Jochemsen, P. J. van den Elsen, R. J. DeLeys, J. Maat, C. P. van Beveren, R. Dijkema, and A. de Waard 383
RNA Transcription and Splicing at Early and Intermediate Times after Adenovirus-2 Infection L. T. Chow, J. B. Lewis, and T. R. Broker 401
Biogenesis, Structures, and Sites of Encoding of the 5¢ Termini of Adenovirus-2 mRNAs C. C. Baker and F. B. Ziff 415
Phenotypes of Adenovirus-5 Host-range Mutants for Early mRNA Synthesis A. J. Berk, F. Lee, T. Harrison, J. Williams, and P. A. Sharp 429
Expression of Genes from the Transforming Region of Adenovirus D. J. Spector, D. N. Halbert, L. D. Crossland, and H. J. Raskas 437
Metabolism of mRNA from the Transforming Region of Adenovirus 2 M. C. Wilson, J. R. Nevins, J.-M. Blanchard, H. S. Ginsberg, and J. E. Darnell, Jr 447
Studies on Early Proteins and Transformation Proteins of Human Adenoviruses M. Green, W. S. M. Wold, K. Brackmann, and M. A. Cartas 457 Analysis of Cloned mRNA Sequences from the Transforming Region of Adenovirus
2 M. Perricaudet, G. Akusjärvi, A. Virtanen, and U. Pettersson 471
Transforming Proteins of Human Adenovirus 5: Studies with Infected and Trans- formed Cells N. J. Lassam, S. T. Bayley, and F. L. Graham 477
Organization and Expression of the Left Third of the Genome of Adenovirus J. B. Lewis, H. Esche, J. E. Smart, B. W. Stillman, M. L. Harter, and M. B. Mathews 493
Adenoviral Early Glycoprotein E3-19K and Its Association with Transplantation Antigens H. Persson, S. Kvist, L. Östberg, P. A. Peterson, and L. Philipson 509
Nucleotide Sequences, Integration, and Transcription of the Adenovirus-12 Trans- forming Genes K. Fujinaga, Y. Sawada, Y. Uemizu, T. Yamashita, H. Shimojo, K. Shiroki, H. Sugisaki, K. Sugimoto, and M. Takanami 519
Products of the Adenovirus-12 Transforming Genes and Their Functions K. Shiroki, K. Segawa, I. Saito, H. Shimojo, and K. Fujinaga 533
Transforming Genes of DNA Tumor Viruses and Their Arrangement in Transformed Cells
Viral DNA Sequences in Adenovirus-transformed Cells L. Visser, M. W. van Maar- schalkerweerd, T. H. Rozijn, A. D. C. Wassenaar, A. M. C. B. Reemst, and J. S. Sussenbach 541
Selectivity in Integration Sites of Adenoviral DNA W. Doerfier, S. Stabel, H. Ibelgaufts, D. Sutter, R. Neumann, J. Groneberg, K. H. Scheidtmann, R. Deuring, and U. Winterhoff 551
Methylation of Integrated Viral DNA Sequences in Hamster Cells Transformed by Adenovirus 12 D. Sutter and W. Doerlier 565 Analysis of the Sites of Integration of Viral DNA Sequences in Rat Cells Transformed by Adenovirus 2 or SV40 J. Sambrook, R. Greene, J. Stringer, T. Mitchison, S.-L. Hu, and M. Botchan 569
Mechanism for Circularization of MVM DNA by "Noose" Sliding in a Lassolike Structure: Implication for Integration Y. Aloni, S. Bratosin, O. Laub, and J. Tal 585
Tumorigenicity of Polyoma Virus in Hamsters M. A. Israel, K. Chowdhury, J. Ramseur, K. Chandrasekaran, D. F. Vanderryn, and M. A. Martin 591
Organization and Expression of Integrated Polyoma Virus DNA Sequences in Trans- formed Rodent Cells L. Lania, A. Hayday, G. Biursell, D. Gandini-Attardi, and M. Fried 597
Monkey Cells Transformed by SV40 DNA Fragments: Flat Revertants Synthesize Large and Small T Antigens M. Graessmann, A. Graessmann, and C. Mueller 605
Integration and Excision of Polyoma Virus Genomes C. Basilico, D. Zouzias, G. Della-Valle, S. Gattoni, V. Colantouni, R. Fenton, and L. Dailey Rapid Detection, Isolation, and Amplification of Host-substituted SV40 Variants F. Winocour, M. Singer, and E. Kuff 621
Morphological Transformation by DNA Fragments of Human Herpesviruses: Evi- dence for Two Distinct Transforming Regions in Herpes Simplex Virus Types 1 and 2 and Lack of Correlation with Biochemical Transfer of the Thymidine Kinase Gene G. R. Reyes, R. LaFemina, S. D. Hayward, and G. S. Hayward 629
The Transformed Cell
Sarcoma Growth Factor: Specific Binding to and Elution from Membrane Receptors for Epidermal Growth Factor J. F. DeLarco and G. J. Todaro 643
Transformation-specific Secreted Proteins D. R. Senger, D. F. Wirth, C. Bryant, and R. O. Hynes 651
A Common Transformation-related Protein in Murine Sarcomas and Leukemias G. Jay, A. B. DeLeo, E. Appella, G. C. Dubois, L. W. Law, G. Khoury, and L. J. Old 659
Regulation of Plasminogen Activator Synthesis in Chick Embryo Fibroblasts Infected with Avian Retroviruses D. B. Rifkin 665
New Assay for the Plasminogen Activator Activity of Transformed Cells W. F. Mangel, D. C. Livingston, J. R. Brocklehurst, H.-Y. Liu, G. A. Peltz, J. F. Cannon, S. P. Leytus, J. A. Wehrly, B. L. Salter, and J. L. Mosher 669
SV40 and Cellular Gene Expression in the Maintenance of the Tumorigenic Syn- drome R. Pollack, A. Lo, B. Steinberg, K. Smith, H. Shure, G. Blanck, and M. Verderame 681
Expression of Transformation in Cell Hybrids: Analysis of a Revertant of SVT2 K. K. Jha, F. G. Gurney, L. A. Feldman, and H. L. Ozer 689
Tumor Formation by Transformed Cells in Nude Mice P. Kahn, R. S. Simon, A. S. Klein, and S. Shin 695
A Study to Determine the Reasons for Differences in the Tumorigenicity of Rat Cell Lines Transformed by Adenovirus 2 and Adenovirus 12 P. H. Gallimore and C. Paraskeva 703
Monoclonal Antibodies: Detection of Transformation-related Antigens S. S. Ved- Brat, U. Hammerling, W. D. Hardy, F. Borenfreund, and W. Prensky 715
PART 2
RNA TUMOR VIRUSES
The Genomes and Proteins of Retroviruses
Organization of Murine Sarcoma Virus Genomes D. J. Donoghue, M. P. Goldfarb, P. A. Sharp, and R. A. Weinberg 721
Cellular Origin of the Transforming Gene of Moloney Murine Sarcoma Virus E. Canaani, S. R. Tronick, K. C. Robbins, P. R. Andersen, C. Y. Dunn, and S. A. Aaronson 727
Characterization of Integrated Moloney Sarcoma Proviruses and Flanking Host Sequences Cloned in Bacteriophage l G. W. Vande Woude, M. Oskarsson, W. L. McClements, L. W. Enquist, D. Blair, P. J. Fischinger, J. V. Maizal, and M. Sullivan 735
Characterization of Virus-specified Proteins Present in NRK Cells Infected with a Temperature-sensitive Transformation Mutant of Moloney Murine Sarcoma Virus T. G. Wood, J. Peltier-Horn, W. G. Robey, D. G. Blair, and R. B. Arlinghaus 747
Comparative Analysis of Cellular and Viral Sequences Related to Sarcomagenic Cell Transformation L.-H. Wang, P. Snyder, T. Hanafusa, C. Moscovici, and H. Hanafusa 755
Transforming Protein Encoded by the Cellular Information of Recovered Avian Sarcoma Viruses R. E. Karess, W. S. Hayward, and H. Hanafusa 765
Correlation of Transient Accumulation of Linear Unintegrated Viral DNA and Transient Cell Killing by Avian Leukosis and Reticuloendotheliosis Vimses H. M. Temin, E. Keshet, and S. K. Weller 773
Endogenous Virogenes and Oncogenes in Rat-cell Transformation: A New Model System S. Rasheed 779
Isolation and Characterization of Proteins Carrying the Feline Oncornavirus-asso- ciated Cell-membrane Antigen H. W. Snyder, Jr., K. J. Phillips, W. D. Hardy, Jr., F. E. Zuckerman, M. Essex, A. H. Sliski, and J. Rhim 787
Genetic Structure of Avian Acute Leukemia Viruses K. Bister and P. H. Duesberg 801
RNAs of Defective and Nondefective Components of Friend Anemia and Poly- cythemia Virus Strains Identified and Compared L. Evans, M. Nunn, P. Dues- berg, D. Troxler, and F. Scolnick 823
Analysis of RNA Specific for the Transforming Agent of R-strain Avian Eryth- roblastosis Viruses K. Toyoshima, T. Kamahora, H. Sugiyama, M. Owada, A. Nomoto, and M. Yoshida 837
Characterization of the src Gene of a Rous Sarcoma Virus Mutant Temperature- sensitive for Cell Transformation J.-L. Darlix, P. A. Bromley, and P.-F. Spahr 843
Structure and Expression of the Abelson Murine Leukemia Virus Genome and Its Relationship to a Normal Cell Gene D. Baltimore, A. Shields, G. Otto, S. Goff, P. Besmer, O. Witte, and N. Rosenberg 849
A Trans-acting Protein Kinase Identified in Cells Transformed by Abelson Murine Leukemia Virus O. N. Witte, L. Sun, N. Rosenberg, and D. Baltimore 855
Characterization of Abelson Murine Leukemia Virus Isolates Differing in P120 Expression N. Rosenberg ,O. N. Witte, and D. Baltimore 859
Mammalian RNA Type-C Transforming Viruses: Characterization of Virus-coded Polyproteins Containing Phosphorylated Components with Possible Transforming Functions J. R. Stephenson, W. J. M. Van de Ven, A. S. Khan, and F. H. Reynolds, Jr 865
RNA Sequences and Proteins Specific to Friend Strain of Spleen Focus-forming Virus Y. Ikawa, Y. Kobayashi, M. Obinata, F. Harada, S. Hino, and H. Yoshikura 875
Progressive Shutdown of Viral Gene Expression in Friend-virus-induced Eryth- roleukemia Cells A. S. Berkower, F. Plata, R. Anand, F. Lilly, R. Soeiro, and R. A. Steeves 887
Host Control of Sequences Specific to Friend Erythroleukemia Virus in Normal and Leukemic Mice T. W. Mak, C. L. Gamble, M. E. MacDonald, and A. Bernstein 893
Moloney Murine Sarcoma Virions Contain Subgenomic-length mRNA-like Molecules That Direct the Synthesis of 'Sarcoma-specific' Polypeptides In Vitro D. Dina and B. Nadal-Ginard 901
src
Protein Phosphorylation Mediated by Partially Purified Avian Sarcoma Virus Transforming-gene Product R. L. Erikson, M. S. Collett, E. Erikson, A. F. Purchio, and J. S. Brugge 907
Origin and Function of Avian Retrovirus Transforming Genes J. M. Bishop, S. A. Courtneidge, A. D. Levinson, H. Oppermann, N. Quintrell, D. K. Sheiness, S. R. Weiss, and H. E. Varmus 919
Studies on the Structure and Function of the Avian Sarcoma Virus Transforming-gene Product T. Hunter, B. M. Sefton, and K. Beemon 931
In Vitro Synthesis and Processing of Rous Sarcoma Virus src-gene Products J.
Kamine, J. G. Burr, and J. M. Buchanan 943 Activities of the src -gene Product of Avian Sarcoma Virus P. F. Maness, H. Engeser, M. F. Greenberg, M. O'Farrell, W. E. Gall, and G. M. Edelman 949
Comparison of Protein Kinase Activities in Normal Cells and Cells Transformed by a Temperature-sensitive Mutant of Avian Sarcoma Virus to Those of Cell-free Viral Translational Products M. Owada, P. Donner, K. E. J. Dittmar, and K. Moelling 959
F-actin Inhibits Protein Kinase Activity Associated with the src-gene Product S. D. Tsen, R. Lee, B. Damiani, P. Hsieh, and L. B. Chen 967
Transformation by Rous Sarcoma Virus: Effects of src-gene Expression on the Synthesis and Phosphorylation of Cellular Polypeptides K. Radke and G. S. Martin 975
Expression of Viral Oncogenes in Differentiating Chick Embryo Neuroretinal Cells Infected with Avian Tumor Viruses G. Calothy, F. Poirier, G. Dambrine, P. Mignatti, P. Combes, and B. Pessac 983
Localization and Characterization of the src -gene Product of Rous Sarcoma Virus A. R. Goldberg, J. G. Krueger, and E. Wang 991
Transformation-defective, Temperature-sensitive Mutants of Rous Sarcoma Virus Have a Reversibly Defective src-gene Product R. R. Friis, B. M. Jockusch, C. B. Boschek, A. Ziemiecki, H. Rübsamen, and H. Bauer 1007
Localization of pp60src within Normal Rat Kidney Cells Infected with Temperature- sensitive Mutants (T-class) of Rous Sarcoma Vims L. R. Rohrschneider 1013
Nature and Intracellular Location of the Product of the src Gene of Avian Sarcoma Virus I. Pastan, G. Jay, N. Richert, P. J. A. Davies, and M. Willingham 1023
Genetic and Biochemical Approaches to Analyzing Transformation by Rous Sarcoma Virus D. D. Anderson, D. W. Salter, L. R. Rohrschneider, and M. J. Weber 1031
Isolation of a Glucose Transport System from Normal Chicken Fibroblasts and from Chicken Fibroblasts Transformed by Rous Sarcoma Virus F. Lipmann and S. G. Lee 1043
Growth-Related Alterations Induced in Chick Embryo Fibroblasts by src-gene Deletion Mutants of the Schmidt-Ruppin Strain of Rous Sarcoma Virus M. L. Breitman, R. Vigne, and P. K. Vogt 1047
Expression of the src-gene Product, pp60src, in Transformed and Reverted Mammalian Cells Infected with Avian Sarcoma Virus A. F. Lau, R. A. Krzyzek, J. S. Brugge, M. S. Collett, R. L. Erikson, and A. J. Faras 1057
Factors Affecting Phenotypic Reversion of Rat Cells Transformed by Avian Sarcoma Virus J. A. Wyke, J. A. Beamand, and H. E. Varmus 1065
Proviruses and Their Expression
Organization of Endogenous and Exogenous Viral and Linked Nonviral Sequences S. H. Hughes, P. K. Vogt, E. Stubblefield, H. Robinson, J. M. Bishop, and H. E. Varmus 1077
Synthesis and Integration of Avian Sarcoma Virus DNA J. M. Taylor, T. W. Hsu, C. Yeater, and W. S. Mason 1091
Avian Endogenous Proviral DNA: Analysis of Integrated ev 1 and a Related gs¯chf¯ Provirus Purified by Molecular Cloning A. Skalka, P. DeBona, F. Hishinuma, and W. McClements 1097
Ten Genetic Loci in the Chicken That Contain Structural Genes for Endogenous Avian Leukosis Viruses S. M. Astrin, H. L. Robinson, L. B. Crittenden, E. G. Buss, J. Wyban, and W. S. Hayward 1105
Transcriptional Products and DNA Structure of Endogenous Avian Proviruses W. S. Hayward, S. B. Braverman, and S. M. Astrin 1111
Role of the C Region in Relative Growth Rates of Endogenous and Exogenous Avian Oncoviruses P. N. Tsichlis and J. M. Coffin 1123
Subgroup-E Avian-leukosis-virus-associated Disease in Chickens H. L. Robinson, M. N. Pearson, D. W. DeSimone, P. N. Tsichlis, and J. M. Coffin 1133
Structure and Infectivity of Integrated Murine Leukemia Virus DNA D. R. Lowy, E. Rands, S. K. Chattopadhyay, and G. L. Hager 1143
Construction and Characterization of Molecular Clones Containing Integrated Mouse Mammary Tumor Virus Sequences L. A. Donehower, J. Andre, D. S. Berard, R. G. Wolford, and G. L. Hager 1153
Genomic Location of Mouse Mammary Tumor Virus Proviral DNA in Normal Mouse Tissue and in Mammary Tumors N. E. Hynes, B. Groner, H. Diggelmann, R. Van Nie, and R. Michalides 1161
Transformation of NIH-3T3 Mouse Cells by Avian Retroviral DNAs G. M. Cooper, N. G. Copeland, A. D. Zelenetz, and T. Krontiris 1169
The Transformed Phenotype and Differentiation
Control of Proliferation and Differentiation in Cells Transformed by Friend Virus D. Housman, R. Levenson, V. Volloch, A. Tsiftsoglou, J. Gusella, D. Parker, J. Kernen, A. Mitrani, V. Weeks, O. Witte, and P. Besmer 1177
Transformation by Abelson Murine Leukemia Virus: Properties of the Transformed Cells W. C. Raschke 1187
Cell-surface Antigens of Abelson and Friend Murine Leukemia Viruses and of Hematopoietic Differentiation R. Risser, D. Grunwald, C. Sinaiko, and P. Jelen 1195
Normal B-cell Activation Involves Endogenous Retroviral Antigen Expression: Im- plications for Leukemogenesis C. Moroni, J. P. Stoye, J. F. DeLamarter, P. Erb, F. A. Jay, J. Jon gstra, D. Martin, and G. Schumann 1205
In Vitro Transformation of Mouse Hematopoietic Cells by the Spleen Focus-forming Virus W. D. Hankins, S. B. Krantz, T. A. Kost, and M. J. Koury 1211
Three New Types of Viral Oncogenes in Defective Avian Leukemia Viruses. I. Specific Nucleotide Sequences of Cellular Origin Correlate with Specific Trans- formation D. Stéhelin, S. Saule, M. Roussel, A. Sergeant, C. Lagrou, C. Rom - mens, and M. B. Raes 1215
Three New Types of Viral Oncogenes in Defective Avian Leukemia Viruses. II. Biological, Genetic, and Immunochemical Evidence T. Graf, H. Beug, A. von Kirchbach, and M. J. Hayman 1225
Recombination in the Avian Oncoviruses as a Model for the Generation of Defective Transforming Viruses R. N. Eisenman, M. Linial, M. Groudine, R. Shaikh, S. Brown, and P. E. Neiman 1235
Progenitor-cell Populations Can Be Infected by RNA Tumor Viruses, but Trans- formation is Dependent on the Expression of Specific Differentiated Func- tions D. Boettiger and F. M. Durban 1249
Viruses of Thymic Leukemia
Murine-leukemia-virus-related Cell-surface Antigens as Serological Markers of AKR Ecotropic, Xenotropic, and Dualtropic Viruses P. V. O'Donnell, E. Stockert, Y. Obata, A. B. DeLeo, and L. J. Old 1255
Status of the Association of Mink Cell Focus-forming Viruses with Leukemogenesis W. P. Rowe, M. W. Cloyd, and J. W. Hartley 1265
Analysis of the Genomes of Mink Cell Focus-forming Murine Type-C Viruses: A Progress Report M. L. Lung, C. Hering, J. W. Hartley, W. P. Rowe, and N. Hopkins 1269
Nucleotide Sequence of a Cloned Murine Leukemia Vims DNA Fragment J. G. Sutcliffe, T. M. Shinnick, R. A. Lerner, P. Johnson, and I. M. Verma 1275
Murine Leukemia Virus env-gene Expression in Preleukemic Thymocytes and Leukemia Cells of AKR Strain Mice N. G. Famulari, J.-S. Tung, P. V. O'Don- nell, and E. Fleissner 1281
Evolution of Oncogenic Mouse Type-C Viruses in Cell Culture U. R. Rapp and G. J. Todaro 1289
T-lymphoma Retroviral Receptors and Control of T-lymphoma Cell Proliferation M. S. McGrath, F. Pillemer, D. A. Kooistra, S. Jacobs, L. Jerabek, and I. L. Weissman 1297
Summary
Molecular Biology of Viral Oncogenes P. A. Sharp 1305
DNA TUMOR VIRUSES
Symposium Participants v
Foreword xvii
Viral Oncogenes H. M. Temin 1
Synopses
Transforming Genes of DNA Tumor Viruses L. V. Crawford 9
Transforming Genes of Retroviruses P. H. Duesberg 13
DNA Tumor Viruses: Early RNAs and Early Proteins
Transcription of the SV40 Genome in Virus-transformed Cells and Early Lytic Infection P. K. Ghosh, M. Piatak, V. B. Reddy, J. Swinscoe, P. Lebowitz, and S. M. Weissman 31
Regulation of 5V40 Gene Expression through RNA Splicing G. Khoury, J. C. Aiwine, R. Dhar, P. Gruss, C.-J. Lai, S. Segal, and L Seif 41
A Third Splice Site in SV40 Early mRNA D. F. Mark and P. Berg 55
Comparison of Polyoma Virus Transcription in Productively Infected Mouse Cells and Transformed Rodent Cell Lines R. Kamen, J. Favaloro, J. Parker, R. Treisman, L. Lania, M. Fried, and A. Mellor 63
Transcription of SV40 DNA in Transformed Rat Cells M. Grigoryan, P. Chumakov, and E. Lukanidin 77
Expression of Transforming Viral Genes in Semipermissive Cells Transformed by SV40 or Adenovirus Type 2 or Type 5 S. J. Flint and G. A. Beltz 89
Catalytic Properties of the SV40 Large T Antigen R. Tjian, A. Robbins, and R. Clark 103
Enzymatic Activities Associated with the SV40 Large T Antigen J. D. Griffin, G. Spangler, and D. M. Livingston 113
DNA Binding and Sedimentation Properties of SV40 T Antigens Synthesized In Vivo and In Vitro C. Prives, Y. Beck, D. Gidoni, M. Oren, and H. Shure 123
Organization of T Antigens in the Polyoma Virus Genome T. Hunter, M. A. Hutchinson, W. Eckhart, T. Friedmann, A. Esty, P. La Porte, and P. Deininger 131
Is Polyoma Virus Middle T Antigen a Protein Kinase? A. E. Smith, M. Fried, Y. Ito, N. Spurr, and R. Smith 141
T Antigens
Polyoma Virus T Antigens Expressed in Transformed Cells: Significance of Middle T Antigen in Transformation Y. Ito and N. Spurr 149
Purification and Characterization of the SV40 F-gene Protein P. Tegtmeyer, T. Spillman, and F. R. Schuetz 159
Phosphorylation of SV40 Large T Antigen G. Walter and P. J. Flory, Jr 165
T Antigens of SV40-transformed Cells F. McCormick, F. Chaudry, R. Harvey, R. Smith, P. W. J. Rigby, E. Paucha, and A. F. Smith 171
Characterization of the Complex between SV40 Large T Antigen and the 53K Host Protein in Transformed Mouse Cells L. V. Crawford, D. P. Lane, D. T. Denhardt, E. F. Harlow, P. M. Nicklin, K. Osborn, and D. C. Pim 179
New Genetic Information Expressed in SV40-transformed Cells: Characterization of the 55K Proteins and Evidence for Unusual SV40 mRNAs P. May, M. Kress, M. Lange, and F. May 189
T-antigen-associated Proteins Induced by SV40 Transformation J. A. Melero, D. S. Greenspan, and R. B. Carroll 201
SV40 17K Protein Is Associated with Two Cellular Proteins K. Rundell, P. Hearing, and Y. C. Yang 211
Characterization of a Murine Cellular SV40 Antigen in SV40-transformed Cells and in Uninfected Embryonal Carcinoma Cells D. I. H. Linzer, W. Maltzman, and A. J. Levine 215
SV40 T-antigen-related Molecules on the Surfaces of HeLa Cells Infected with Adenovirus-2-SV40 Hybrids and on SV40-transformed Cells W. Deppert and R. Henning 225
SV40 Transplantation Antigen: Relationship to SV40-specific Proteins S. S. Tevethia, R. S. Greenfield, D. C. Flyer, and M. J. Tevethia 235
Host Antigens in the Centriolar Region Are Induced in SV40-infected TC7 Cells: SV40 Small-T-function Requirement H. Kasamatsu, M. Shyamala, and W. Lin 243
Transformation Induced by Epstein-Barr Virus and the Role of the Nuclear Antigen G. Klein, J. Luka, and J. Zeuthen 253
The Genetics of Transformation by DNA Tumor Viruses
The hr-t Gene of Polyoma Virus T. L. Benjamin, G. G. Carmichael, and B. S. Schaffhausen 263
Early Mutants of Polyoma Virus (dl8 and dl23) with Altered Transformation Proper- ties: Is Polyoma Virus Middle T Antigen a Transforming Gene Product? B. E. Griffin, Y. Ito, U. Novak, N. Spurr, S. Dilworth, N. Smolar, R. Pollack, K. Smith, and D. Rifkin 271
Deletion Mutants of SV4O That Affect the Structure of Viral Tumor Antigens J. M. Pipas, S. P. Adler, K. W. C. Peden, and D. Nathans 285
Origin-defective Mutants of SV40 Y. Gluzman, R. J. Frisque, and 1. Sambrook 293
Lytic Infection of Teratocarcinoma Cells by Polyoma Virus Mutants M. Vasseur, C. Kress, N. Montreau, and D. Blangy 301
SV40 Mutant tsA 1499 Is Heat-sensitive for Lytic Growth but Generates Cold-sensitive Rat-cell Transformants D. Pintel, N. Bouck, G. di Mayorca, B. Thimmappaya, B. Swerdlow, and T. Shenk 305
Roles of the T Antigens in Transformation by SV40 R. G. Martin, V. P. Setlow, A. B. Chepelinsky, R. Seif, A. M. Lewis, Jr., C. D. Scher, C. D. Stiles, and J. Avila 311
Requirement for the Large T and Small T Proteins of 5V40 in the Maintenance of the Transformed State R. J. Frisque, D. B. Rifkin, and W. C. Topp 325
Host-virus Interactions Critical for Cellular Transformation by Polyoma Virus and SV40 M. Rassoulzadegan, E. Mougneau, B. Perbal, P. Gaudray, F. Birg, and F. Cuzin 333
Viral and Cellular Control of the SV40-transformed Phenotype J. B. Hiscott and V. Defendi 343
Location of Additional Early Regions within the Left Quarter of the Adenoviral Genome J. Williams, R. S. Galos, M. H. Binger, and S. J. Flint 353
Functional Analysis of Adenovirus-5 Host-range Deletion Mutants Defective for Transformation of Rat Embryo Cells T. Shenk, N. Jones, W. Colby, and D. Fowlkes 367
SV-induced Somatic Mutations: Possible Relevance to Viral Transformation M. Theile, M. Strauss, L. Luebbe, S. Scherneck, H. Krause, and F. Geissler 377
The Transforming Genes of Adenoviruses and Their Expression
Structure and Function of the Transforming Genes of Human Adenoviruses and SV40 AJ. van der Eb, H. van Ormondt, P. I. Schrier, J. H. Lupker, H. Jochemsen, P. J. van den Elsen, R. J. DeLeys, J. Maat, C. P. van Beveren, R. Dijkema, and A. de Waard 383
RNA Transcription and Splicing at Early and Intermediate Times after Adenovirus-2 Infection L. T. Chow, J. B. Lewis, and T. R. Broker 401
Biogenesis, Structures, and Sites of Encoding of the 5¢ Termini of Adenovirus-2 mRNAs C. C. Baker and F. B. Ziff 415
Phenotypes of Adenovirus-5 Host-range Mutants for Early mRNA Synthesis A. J. Berk, F. Lee, T. Harrison, J. Williams, and P. A. Sharp 429
Expression of Genes from the Transforming Region of Adenovirus D. J. Spector, D. N. Halbert, L. D. Crossland, and H. J. Raskas 437
Metabolism of mRNA from the Transforming Region of Adenovirus 2 M. C. Wilson, J. R. Nevins, J.-M. Blanchard, H. S. Ginsberg, and J. E. Darnell, Jr 447
Studies on Early Proteins and Transformation Proteins of Human Adenoviruses M. Green, W. S. M. Wold, K. Brackmann, and M. A. Cartas 457 Analysis of Cloned mRNA Sequences from the Transforming Region of Adenovirus
2 M. Perricaudet, G. Akusjärvi, A. Virtanen, and U. Pettersson 471
Transforming Proteins of Human Adenovirus 5: Studies with Infected and Trans- formed Cells N. J. Lassam, S. T. Bayley, and F. L. Graham 477
Organization and Expression of the Left Third of the Genome of Adenovirus J. B. Lewis, H. Esche, J. E. Smart, B. W. Stillman, M. L. Harter, and M. B. Mathews 493
Adenoviral Early Glycoprotein E3-19K and Its Association with Transplantation Antigens H. Persson, S. Kvist, L. Östberg, P. A. Peterson, and L. Philipson 509
Nucleotide Sequences, Integration, and Transcription of the Adenovirus-12 Trans- forming Genes K. Fujinaga, Y. Sawada, Y. Uemizu, T. Yamashita, H. Shimojo, K. Shiroki, H. Sugisaki, K. Sugimoto, and M. Takanami 519
Products of the Adenovirus-12 Transforming Genes and Their Functions K. Shiroki, K. Segawa, I. Saito, H. Shimojo, and K. Fujinaga 533
Transforming Genes of DNA Tumor Viruses and Their Arrangement in Transformed Cells
Viral DNA Sequences in Adenovirus-transformed Cells L. Visser, M. W. van Maar- schalkerweerd, T. H. Rozijn, A. D. C. Wassenaar, A. M. C. B. Reemst, and J. S. Sussenbach 541
Selectivity in Integration Sites of Adenoviral DNA W. Doerfier, S. Stabel, H. Ibelgaufts, D. Sutter, R. Neumann, J. Groneberg, K. H. Scheidtmann, R. Deuring, and U. Winterhoff 551
Methylation of Integrated Viral DNA Sequences in Hamster Cells Transformed by Adenovirus 12 D. Sutter and W. Doerlier 565 Analysis of the Sites of Integration of Viral DNA Sequences in Rat Cells Transformed by Adenovirus 2 or SV40 J. Sambrook, R. Greene, J. Stringer, T. Mitchison, S.-L. Hu, and M. Botchan 569
Mechanism for Circularization of MVM DNA by "Noose" Sliding in a Lassolike Structure: Implication for Integration Y. Aloni, S. Bratosin, O. Laub, and J. Tal 585
Tumorigenicity of Polyoma Virus in Hamsters M. A. Israel, K. Chowdhury, J. Ramseur, K. Chandrasekaran, D. F. Vanderryn, and M. A. Martin 591
Organization and Expression of Integrated Polyoma Virus DNA Sequences in Trans- formed Rodent Cells L. Lania, A. Hayday, G. Biursell, D. Gandini-Attardi, and M. Fried 597
Monkey Cells Transformed by SV40 DNA Fragments: Flat Revertants Synthesize Large and Small T Antigens M. Graessmann, A. Graessmann, and C. Mueller 605
Integration and Excision of Polyoma Virus Genomes C. Basilico, D. Zouzias, G. Della-Valle, S. Gattoni, V. Colantouni, R. Fenton, and L. Dailey Rapid Detection, Isolation, and Amplification of Host-substituted SV40 Variants F. Winocour, M. Singer, and E. Kuff 621
Morphological Transformation by DNA Fragments of Human Herpesviruses: Evi- dence for Two Distinct Transforming Regions in Herpes Simplex Virus Types 1 and 2 and Lack of Correlation with Biochemical Transfer of the Thymidine Kinase Gene G. R. Reyes, R. LaFemina, S. D. Hayward, and G. S. Hayward 629
The Transformed Cell
Sarcoma Growth Factor: Specific Binding to and Elution from Membrane Receptors for Epidermal Growth Factor J. F. DeLarco and G. J. Todaro 643
Transformation-specific Secreted Proteins D. R. Senger, D. F. Wirth, C. Bryant, and R. O. Hynes 651
A Common Transformation-related Protein in Murine Sarcomas and Leukemias G. Jay, A. B. DeLeo, E. Appella, G. C. Dubois, L. W. Law, G. Khoury, and L. J. Old 659
Regulation of Plasminogen Activator Synthesis in Chick Embryo Fibroblasts Infected with Avian Retroviruses D. B. Rifkin 665
New Assay for the Plasminogen Activator Activity of Transformed Cells W. F. Mangel, D. C. Livingston, J. R. Brocklehurst, H.-Y. Liu, G. A. Peltz, J. F. Cannon, S. P. Leytus, J. A. Wehrly, B. L. Salter, and J. L. Mosher 669
SV40 and Cellular Gene Expression in the Maintenance of the Tumorigenic Syn- drome R. Pollack, A. Lo, B. Steinberg, K. Smith, H. Shure, G. Blanck, and M. Verderame 681
Expression of Transformation in Cell Hybrids: Analysis of a Revertant of SVT2 K. K. Jha, F. G. Gurney, L. A. Feldman, and H. L. Ozer 689
Tumor Formation by Transformed Cells in Nude Mice P. Kahn, R. S. Simon, A. S. Klein, and S. Shin 695
A Study to Determine the Reasons for Differences in the Tumorigenicity of Rat Cell Lines Transformed by Adenovirus 2 and Adenovirus 12 P. H. Gallimore and C. Paraskeva 703
Monoclonal Antibodies: Detection of Transformation-related Antigens S. S. Ved- Brat, U. Hammerling, W. D. Hardy, F. Borenfreund, and W. Prensky 715
PART 2
RNA TUMOR VIRUSES
The Genomes and Proteins of Retroviruses
Organization of Murine Sarcoma Virus Genomes D. J. Donoghue, M. P. Goldfarb, P. A. Sharp, and R. A. Weinberg 721
Cellular Origin of the Transforming Gene of Moloney Murine Sarcoma Virus E. Canaani, S. R. Tronick, K. C. Robbins, P. R. Andersen, C. Y. Dunn, and S. A. Aaronson 727
Characterization of Integrated Moloney Sarcoma Proviruses and Flanking Host Sequences Cloned in Bacteriophage l G. W. Vande Woude, M. Oskarsson, W. L. McClements, L. W. Enquist, D. Blair, P. J. Fischinger, J. V. Maizal, and M. Sullivan 735
Characterization of Virus-specified Proteins Present in NRK Cells Infected with a Temperature-sensitive Transformation Mutant of Moloney Murine Sarcoma Virus T. G. Wood, J. Peltier-Horn, W. G. Robey, D. G. Blair, and R. B. Arlinghaus 747
Comparative Analysis of Cellular and Viral Sequences Related to Sarcomagenic Cell Transformation L.-H. Wang, P. Snyder, T. Hanafusa, C. Moscovici, and H. Hanafusa 755
Transforming Protein Encoded by the Cellular Information of Recovered Avian Sarcoma Viruses R. E. Karess, W. S. Hayward, and H. Hanafusa 765
Correlation of Transient Accumulation of Linear Unintegrated Viral DNA and Transient Cell Killing by Avian Leukosis and Reticuloendotheliosis Vimses H. M. Temin, E. Keshet, and S. K. Weller 773
Endogenous Virogenes and Oncogenes in Rat-cell Transformation: A New Model System S. Rasheed 779
Isolation and Characterization of Proteins Carrying the Feline Oncornavirus-asso- ciated Cell-membrane Antigen H. W. Snyder, Jr., K. J. Phillips, W. D. Hardy, Jr., F. E. Zuckerman, M. Essex, A. H. Sliski, and J. Rhim 787
Genetic Structure of Avian Acute Leukemia Viruses K. Bister and P. H. Duesberg 801
RNAs of Defective and Nondefective Components of Friend Anemia and Poly- cythemia Virus Strains Identified and Compared L. Evans, M. Nunn, P. Dues- berg, D. Troxler, and F. Scolnick 823
Analysis of RNA Specific for the Transforming Agent of R-strain Avian Eryth- roblastosis Viruses K. Toyoshima, T. Kamahora, H. Sugiyama, M. Owada, A. Nomoto, and M. Yoshida 837
Characterization of the src Gene of a Rous Sarcoma Virus Mutant Temperature- sensitive for Cell Transformation J.-L. Darlix, P. A. Bromley, and P.-F. Spahr 843
Structure and Expression of the Abelson Murine Leukemia Virus Genome and Its Relationship to a Normal Cell Gene D. Baltimore, A. Shields, G. Otto, S. Goff, P. Besmer, O. Witte, and N. Rosenberg 849
A Trans-acting Protein Kinase Identified in Cells Transformed by Abelson Murine Leukemia Virus O. N. Witte, L. Sun, N. Rosenberg, and D. Baltimore 855
Characterization of Abelson Murine Leukemia Virus Isolates Differing in P120 Expression N. Rosenberg ,O. N. Witte, and D. Baltimore 859
Mammalian RNA Type-C Transforming Viruses: Characterization of Virus-coded Polyproteins Containing Phosphorylated Components with Possible Transforming Functions J. R. Stephenson, W. J. M. Van de Ven, A. S. Khan, and F. H. Reynolds, Jr 865
RNA Sequences and Proteins Specific to Friend Strain of Spleen Focus-forming Virus Y. Ikawa, Y. Kobayashi, M. Obinata, F. Harada, S. Hino, and H. Yoshikura 875
Progressive Shutdown of Viral Gene Expression in Friend-virus-induced Eryth- roleukemia Cells A. S. Berkower, F. Plata, R. Anand, F. Lilly, R. Soeiro, and R. A. Steeves 887
Host Control of Sequences Specific to Friend Erythroleukemia Virus in Normal and Leukemic Mice T. W. Mak, C. L. Gamble, M. E. MacDonald, and A. Bernstein 893
Moloney Murine Sarcoma Virions Contain Subgenomic-length mRNA-like Molecules That Direct the Synthesis of 'Sarcoma-specific' Polypeptides In Vitro D. Dina and B. Nadal-Ginard 901
src
Protein Phosphorylation Mediated by Partially Purified Avian Sarcoma Virus Transforming-gene Product R. L. Erikson, M. S. Collett, E. Erikson, A. F. Purchio, and J. S. Brugge 907
Origin and Function of Avian Retrovirus Transforming Genes J. M. Bishop, S. A. Courtneidge, A. D. Levinson, H. Oppermann, N. Quintrell, D. K. Sheiness, S. R. Weiss, and H. E. Varmus 919
Studies on the Structure and Function of the Avian Sarcoma Virus Transforming-gene Product T. Hunter, B. M. Sefton, and K. Beemon 931
In Vitro Synthesis and Processing of Rous Sarcoma Virus src-gene Products J.
Kamine, J. G. Burr, and J. M. Buchanan 943 Activities of the src -gene Product of Avian Sarcoma Virus P. F. Maness, H. Engeser, M. F. Greenberg, M. O'Farrell, W. E. Gall, and G. M. Edelman 949
Comparison of Protein Kinase Activities in Normal Cells and Cells Transformed by a Temperature-sensitive Mutant of Avian Sarcoma Virus to Those of Cell-free Viral Translational Products M. Owada, P. Donner, K. E. J. Dittmar, and K. Moelling 959
F-actin Inhibits Protein Kinase Activity Associated with the src-gene Product S. D. Tsen, R. Lee, B. Damiani, P. Hsieh, and L. B. Chen 967
Transformation by Rous Sarcoma Virus: Effects of src-gene Expression on the Synthesis and Phosphorylation of Cellular Polypeptides K. Radke and G. S. Martin 975
Expression of Viral Oncogenes in Differentiating Chick Embryo Neuroretinal Cells Infected with Avian Tumor Viruses G. Calothy, F. Poirier, G. Dambrine, P. Mignatti, P. Combes, and B. Pessac 983
Localization and Characterization of the src -gene Product of Rous Sarcoma Virus A. R. Goldberg, J. G. Krueger, and E. Wang 991
Transformation-defective, Temperature-sensitive Mutants of Rous Sarcoma Virus Have a Reversibly Defective src-gene Product R. R. Friis, B. M. Jockusch, C. B. Boschek, A. Ziemiecki, H. Rübsamen, and H. Bauer 1007
Localization of pp60src within Normal Rat Kidney Cells Infected with Temperature- sensitive Mutants (T-class) of Rous Sarcoma Vims L. R. Rohrschneider 1013
Nature and Intracellular Location of the Product of the src Gene of Avian Sarcoma Virus I. Pastan, G. Jay, N. Richert, P. J. A. Davies, and M. Willingham 1023
Genetic and Biochemical Approaches to Analyzing Transformation by Rous Sarcoma Virus D. D. Anderson, D. W. Salter, L. R. Rohrschneider, and M. J. Weber 1031
Isolation of a Glucose Transport System from Normal Chicken Fibroblasts and from Chicken Fibroblasts Transformed by Rous Sarcoma Virus F. Lipmann and S. G. Lee 1043
Growth-Related Alterations Induced in Chick Embryo Fibroblasts by src-gene Deletion Mutants of the Schmidt-Ruppin Strain of Rous Sarcoma Virus M. L. Breitman, R. Vigne, and P. K. Vogt 1047
Expression of the src-gene Product, pp60src, in Transformed and Reverted Mammalian Cells Infected with Avian Sarcoma Virus A. F. Lau, R. A. Krzyzek, J. S. Brugge, M. S. Collett, R. L. Erikson, and A. J. Faras 1057
Factors Affecting Phenotypic Reversion of Rat Cells Transformed by Avian Sarcoma Virus J. A. Wyke, J. A. Beamand, and H. E. Varmus 1065
Proviruses and Their Expression
Organization of Endogenous and Exogenous Viral and Linked Nonviral Sequences S. H. Hughes, P. K. Vogt, E. Stubblefield, H. Robinson, J. M. Bishop, and H. E. Varmus 1077
Synthesis and Integration of Avian Sarcoma Virus DNA J. M. Taylor, T. W. Hsu, C. Yeater, and W. S. Mason 1091
Avian Endogenous Proviral DNA: Analysis of Integrated ev 1 and a Related gs¯chf¯ Provirus Purified by Molecular Cloning A. Skalka, P. DeBona, F. Hishinuma, and W. McClements 1097
Ten Genetic Loci in the Chicken That Contain Structural Genes for Endogenous Avian Leukosis Viruses S. M. Astrin, H. L. Robinson, L. B. Crittenden, E. G. Buss, J. Wyban, and W. S. Hayward 1105
Transcriptional Products and DNA Structure of Endogenous Avian Proviruses W. S. Hayward, S. B. Braverman, and S. M. Astrin 1111
Role of the C Region in Relative Growth Rates of Endogenous and Exogenous Avian Oncoviruses P. N. Tsichlis and J. M. Coffin 1123
Subgroup-E Avian-leukosis-virus-associated Disease in Chickens H. L. Robinson, M. N. Pearson, D. W. DeSimone, P. N. Tsichlis, and J. M. Coffin 1133
Structure and Infectivity of Integrated Murine Leukemia Virus DNA D. R. Lowy, E. Rands, S. K. Chattopadhyay, and G. L. Hager 1143
Construction and Characterization of Molecular Clones Containing Integrated Mouse Mammary Tumor Virus Sequences L. A. Donehower, J. Andre, D. S. Berard, R. G. Wolford, and G. L. Hager 1153
Genomic Location of Mouse Mammary Tumor Virus Proviral DNA in Normal Mouse Tissue and in Mammary Tumors N. E. Hynes, B. Groner, H. Diggelmann, R. Van Nie, and R. Michalides 1161
Transformation of NIH-3T3 Mouse Cells by Avian Retroviral DNAs G. M. Cooper, N. G. Copeland, A. D. Zelenetz, and T. Krontiris 1169
The Transformed Phenotype and Differentiation
Control of Proliferation and Differentiation in Cells Transformed by Friend Virus D. Housman, R. Levenson, V. Volloch, A. Tsiftsoglou, J. Gusella, D. Parker, J. Kernen, A. Mitrani, V. Weeks, O. Witte, and P. Besmer 1177
Transformation by Abelson Murine Leukemia Virus: Properties of the Transformed Cells W. C. Raschke 1187
Cell-surface Antigens of Abelson and Friend Murine Leukemia Viruses and of Hematopoietic Differentiation R. Risser, D. Grunwald, C. Sinaiko, and P. Jelen 1195
Normal B-cell Activation Involves Endogenous Retroviral Antigen Expression: Im- plications for Leukemogenesis C. Moroni, J. P. Stoye, J. F. DeLamarter, P. Erb, F. A. Jay, J. Jon gstra, D. Martin, and G. Schumann 1205
In Vitro Transformation of Mouse Hematopoietic Cells by the Spleen Focus-forming Virus W. D. Hankins, S. B. Krantz, T. A. Kost, and M. J. Koury 1211
Three New Types of Viral Oncogenes in Defective Avian Leukemia Viruses. I. Specific Nucleotide Sequences of Cellular Origin Correlate with Specific Trans- formation D. Stéhelin, S. Saule, M. Roussel, A. Sergeant, C. Lagrou, C. Rom - mens, and M. B. Raes 1215
Three New Types of Viral Oncogenes in Defective Avian Leukemia Viruses. II. Biological, Genetic, and Immunochemical Evidence T. Graf, H. Beug, A. von Kirchbach, and M. J. Hayman 1225
Recombination in the Avian Oncoviruses as a Model for the Generation of Defective Transforming Viruses R. N. Eisenman, M. Linial, M. Groudine, R. Shaikh, S. Brown, and P. E. Neiman 1235
Progenitor-cell Populations Can Be Infected by RNA Tumor Viruses, but Trans- formation is Dependent on the Expression of Specific Differentiated Func- tions D. Boettiger and F. M. Durban 1249
Viruses of Thymic Leukemia
Murine-leukemia-virus-related Cell-surface Antigens as Serological Markers of AKR Ecotropic, Xenotropic, and Dualtropic Viruses P. V. O'Donnell, E. Stockert, Y. Obata, A. B. DeLeo, and L. J. Old 1255
Status of the Association of Mink Cell Focus-forming Viruses with Leukemogenesis W. P. Rowe, M. W. Cloyd, and J. W. Hartley 1265
Analysis of the Genomes of Mink Cell Focus-forming Murine Type-C Viruses: A Progress Report M. L. Lung, C. Hering, J. W. Hartley, W. P. Rowe, and N. Hopkins 1269
Nucleotide Sequence of a Cloned Murine Leukemia Vims DNA Fragment J. G. Sutcliffe, T. M. Shinnick, R. A. Lerner, P. Johnson, and I. M. Verma 1275
Murine Leukemia Virus env-gene Expression in Preleukemic Thymocytes and Leukemia Cells of AKR Strain Mice N. G. Famulari, J.-S. Tung, P. V. O'Don- nell, and E. Fleissner 1281
Evolution of Oncogenic Mouse Type-C Viruses in Cell Culture U. R. Rapp and G. J. Todaro 1289
T-lymphoma Retroviral Receptors and Control of T-lymphoma Cell Proliferation M. S. McGrath, F. Pillemer, D. A. Kooistra, S. Jacobs, L. Jerabek, and I. L. Weissman 1297
Summary
Molecular Biology of Viral Oncogenes P. A. Sharp 1305
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