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 XLIII: DNA: Replication and Recombination 1978 |
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From left: Dr. Louise Chow, Dr. Tom Broker  SYMPOSIUM SYNOPSIS LIST OF PARTICIPANTS CONTENTS OF SYMPOSIUM VOLUME PHOTOGRAPHS PUBLISHED VOLUME  |
DNA: Replication and Recombination
Symposium Volume XLIII Contents
PART 1
Symposium Participants v
Foreword xv
Introduction
Aspects of DNA Replication A. Kornberg 1
DNA Unwinding
Structural and Energetic Considerations of Wave Propagation in DNA H. M Sobell, E. D. Lozansky, and M Lessen 11
Gene-V Product of Bacteriophage fd: Structure of a DNA-unwinding Protein and Its Complexes with DNA A. McPherson, F Jumak, A. Wang, F. Kolpak, I. Molineux, and A. Rich 21
DNA: Bihelical Structure, Supercoiling, and Relaxation J. C. Wang 29
DNA Gyrase and DNA Supercoiling M. Gellert, K. Mizuuchi, M. H. O'Dea, H. Ohmori, and J. Tomizawa 35
Structure and Activities of Escherichia coli DNA Gyrase C. L. Peebles, N. P. Higgins, K. N. Kreuzer, A. Morrison, P. O. Brown, A. Sugino, and N. R. Cozzarelli 41
Studies on the Regulation and Specificity of the DNA-untwisting Enzyme J. J. Champoux, L. S. Young, and M D. Been 53
Gene-II Protein of Bacteriophage fd in Enzymatic Replication of Viral Duplex DNA K. Geider and T. F. Meyer 59
DNA Helicases B. Kuhn, M Abdel-Monem, and H. Hoffmann-Berling 63
Replication Origins
Genetic and Physical Studies on the Replication of ColE1-type Plasmids K. Back- man, M Betlach, H. W. Boyer, and S. Yanofsky 69
Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322 J. G. Sutcliffe 77
Plasmid DNA Replication: RK2- and R6K-encoded Trans-acting Factors and Their Sites of Action R.. Kolter, M. Inuzuka, D. Figurski, C. Thomas, D. Stalker, and D. R.. Helinski 91
Essential Regions for Replication of a Stringent and a Relaxed Plasmid in Escherichia coli M. L. Kahn, D. Figurski, L. Ito, and D. R.. Helinski 99
Plasmid-encoded Functions Involved in the Replication and Inheritance of Antibiotic- resistance Plasmid R6-5 K. N. Timmis, I. Andrés, P. M. Slocombe, and R.. M. Synenki 105
Use of Autonomously Replicating Mini-R6K Plasmids in the Analysis of the Replica- tion Regions of the R Plasmid R6K J. H. Crosa, L. K. Luttropp, and S. Falkow 111
Origin of Replication, oriC, of the Escherichia coli K12 Chromosome: Genetic Mapping and Minichromosome Replication K. von Meyenburg, F. G. Hansen, F. Ruse, H. E. N. Bergmans, M. Meijer, and W. Messer 121
Structural and Functional Properties of the Escherichia coli Origin of DNA Replication Y. Hirota, S. Yasuda, M. Yamada, A. Nishimura, K. Sugimoto, H. Sugisaki, A. Oka, and M Takanami 129
Origin of Replication, oriC, of the Escherichia coli K12 Chromosome: Nucleotide Sequence W. Messer, M Meijer, H. F. N. Bergmans, F. G. Hansen, K. von Meyenburg, F. Beck, and H. Schaller 139
Positive and Negative Control of Bacteriophage Lambda DNA Replication M E. Furth, J. L. Yates, and W. F. Dove 147
Dissection and Comparative Anatomy of the Origins of Replication of Lambdoid Phages D. D. Moore, K. Denniston-Thompson, K. E. Kruger, M. E. Furth, B. G. Williams, D. L. Daniels, and F R. Blattner 155
Functional Analysis of the Replicator Structure of Lambdoid Bacteriophage DNAs G. Hobom, R. Grosschede, M. Lusky, G. Scherer, E. Schwarz. and H. Kössel 165
Nucleotide Sequence of a Fragment of HeLa-cell Mitochondrial DNA Containing the Precisely Localized Origin of Replication G. Attardi, S. T. Crews, J. Nishiguchi, D. K. Ojala, and J. W. Posakony 179
Cloning and Characterization of the Replication Origin from Rat Mitochondrial DNA K. Koike, M. Kobayashi, and T. Sekiya 193
Priming and Okazaki Fragments
Structure and Metabolism of the RNA Primer in the Discontinuous Replication of Prokaryotic DNA T. Okazaki, Y. Kurosawa, T. Ogawa, T. Seki, K. Shinozaki, S. Hirose, A. Fujiyama, Y. Kohara, Y. Machida, F. Tamanoi, and T. Hozumi 203
Excision-Repair of Uracil in DNA: Its Implications for the Discontinuous Replication of DNA In Vivo and In Vitro I. R.. Lehman, B.-K. Tye, and P.-O. Nyman 221
Formation of Okazaki Pieces at the Escherichia coli Replication Fork In Vitro K. R.. Thomas, P. Manlapaz-Ramos, R.. Lundquist, and B. M. Olivera 231
Uracil Incorporation into Nascent DNA of Bacillus subtilis and Escherichia coli F Tamanoi, Y. Machida, and T. Okazaki 239
Evidence for Many Nonnascent DNA Molecules the Size of Okazaki Fragments in Escherichia coli and 3T3 Cells: Very Few Have 5¢-Terminal Ribonucleotides D. T. Denhardt, J. Kowaiski, and C. Miyamoto 243
Replication of Mouse L-cell Mitochondrial DNA D. Bogenhagen, A. M. Gillum, P. A. Martens, and D. A. Clayton 253
Studies on Early Intermediates in the Synthesis of DNA in Animal Cells B. Y Tseng, R.. H. Grafstrom, D. Revie, W. Oertel, and M. Goulian 263
Synthesis and Function of Polyoma Initiator RNA P. Reichard and R.. Eliasson 271
Characterization of the 5¢ Ends of Nascent DNA Chains R.. Werner, D. W. Siegmann, A. T. Diaz, and H. Jering 279
Replication of Small Phages and Plasmids
Enzymatic Conversion of Single-stranded f Xl74 and G4 Circles to Duplex Forms: Discontinuous Replication R.. R.. Meyer, J. Shlomai, J. Kobori, D. L. Bates, L. Rowen, R. McMacken, K. Ueda, and A. Kornberg 289
Enzymatic Replication of f X174 Duplex Circles: Continuous Synthesis S. Eisenberg, J. F. Scott. and A. Kornberg 295
DNA Replication Proteins of Escherichia coli and Phage l S. H. Wickner 303
Replication of f X174 DNA: In Vitro Synthesis of f X RFI DNA and Circular, Single-stranded DNA C. Sumida-Yasumoto, J.-E. Ikeda, E. Benz, K. J. Marians, R.. Vicuna, S. Sugrue, S. L. Zipursky, and J. Hurwitz 311
Cleavage Site of f X174 Gene-A Protein in f X and G4 RFI DNA A. D. M. van Mansfeld, S. A. Langeveld, P. J. Weisbeek, P. D. Baas, G. A. van Arkel, and H. S. Jansz 331
The rep Protein of Escherichia coli: Interaction with DNA and Other Proteins M. Duguet. G. Yarranton, and M. Gefter 335
DNA-dependent ATPases from Escherichia coli K12 F. Richet, 1. Moreau, and M Kohiyama 345
Single-stranded Phage Replication: Positive- and Negative-strand DNA Synthesis J. Sims, K. Koths, and D. Dressier 349 G4 DNA Replication In Vivo and the Origins of Its Strand Synthesis G. N. Godson 367
Nucleotide Sequence of the Origin for Bacteriophage M13 DNA Replication S. V. Suggs and D. S. Ray 379
DNA Replication of Bacteriophage fi In Vivo K. Horiuchi, J. V. Ravetch, and D. Zinder 389
The Intergenic Region and the Origins for Filamentous Phage DNA Replication H. Schaller 401
Initiation of Replication of Plasmid CoIEl DNA by RNA Polymerase, Ribonuclease H, and DNA Polymerase I T. Itoh and J. Tomizawa 409
DNA Replication Control: Studies of Plasmid Ri P. Gustafsson, H. Dreisig, S. Molin, K. Nordström, and B. Uhlin 419
Replication of Large Phages and Bacteria
Replication of Bacteriophage T7 DNA by Purified Proteins C. C. Richardson, L. J. Romano, R.. Kolodner, J. E. LeClerc, F. Tamanoi, M. J. Engler, F. B. Dean, and D. S. Richardson 427
Cloning of the T7 Genome in Escherichia coli: Use of Recombination between Cloned Sequences and Bacteriophage T7 to Identify Genes Involved in Recombination and a Clone Containing the Origin of T7 DNA Replication J. L. Campbell, F. Tamanoi, C. C. Richardson, and F. W. Studier 441
Bacteriophage T7 DNA Primase: A Multifunctional Enzyme Involved in DNA Replication G. Hillenbrand, G. Morelli, F. Lanka, and F. Scherzinger 449
Cross-reactivation and Partial Replication of UV-irradiated T7+ DNA K. B. Burck, D. M. Taylor, H W Smith, and R.. C. Miller, Jr. Probing DNA Replication Mechanisms with the T4 Bacteriophage In Vitro System C. C. Liu, R. L. Burke, U Hibner, J. Barry, and B. Alberts 469
DNA Replication by Bacteriophage T4 Proteins: Role of the DNA-delay Gene 61 in the Chain-initiation Reaction L. L. Silver and N. G. Nossal 489
Positive Regulation of T-even-phage DNA Replication by the DNA-delay Protein of Gene 39 W M Huang 495
In Vivo Interactions of Genes and Proteins in DNA Replication and Recombination of Phage T4 G. Mosig, A. Luder, G. Garcia, R.. Dannenberg, and S. Bock Late Replication and Recombination in the Vegetative Pool of T4 N. Hutchinson, T. Kazic, S. J. Lee, C. Rayssiguier, B. S. Emanuel, and W. Kozinski 517
f 29 DNA-Protein Complex and the Structure of Replicating DNA Molecules J. Ito, N. F. Harding, and K. Saigo 525
recA-Dependent DNA Replication in the Absence of Protein Synthesis: Characteris- tics of a Dominant Lethal Replication Mutation, dnaT, and Requirement for recA+ Function K. G. Lark and C. A. Lark 537
A dnaB-Analog DNA-replication Protein of Phage P1 H Schuster, F. Lanka, C Edelbluth, B. Geschke, M. Mikolajczyk, M Schlicht, and D. Touati-Schwartz 551
The Escherichia co/i dnaB Protein Is Required for Initiation of Chromosomal DNA Replication F. K. Fujimura, J. W. Zyskind, and D. W Smith 559
Chromosome Replication in Escherichia coli Is Inhibited in the Terminus Region Near the rac Locus P. L. Kuempel and S. A. Duerr 563
Organization of the Replication-origin Region of the Bacillus subtilis Chromosome H. Yoshikawa, K. Yamaguchi, M. Seiki, N. Ogasawara, and H. Toyoda 569
Replication of Eukaryotic DNA
Studies on the Prereplicative Phase of the Cell Cycle in Saccharomyces cerevisiae D. R. W. Edwards, J. B. Taylor, W. F Wakeling, F. Z. Watts, and I. R. Johnston 577
A Stimulatory Factor for Yeast DNA Polymerase L. M. S. Chang, K. Lurie, and P. Plevani 587
Eukaryotic DNA Replication: The First Steps toward a Multienzyme System from Xenopus laevis R. M Benbow, C. B. Breaux, H. Joenje, M. R. Krauss, R. W. Lennox, F. M. Nelson, N. S. Wang, and S. H. White 597
Two Cell-free Systems Constructed from Xenopus laevis Oocytes: Generalized Genetic Recombination; DNA Supercoiling G. P. Tocchini-Valentini, M. I. Baldi, G. Carrara, D. G. Attardi, and F. Mattoccia 603
Structural and Enzymological Properties of Human DNA Polymerases a and b D. Korn, P. A. Fisher, J. Battey, and T. S.-F. Wang 613
Functions of DNA Polymerases a, b, and g in Neurons during Development U. Hübscher, C. C. Kuenzle, W. Limacher, P. Scherrer, and S. Spadari 625
Studies on the Interaction of tRNA and Avian Myeloblastosis DNA Polymerase A. Araya and S. Litvak 631
Enhancement of the Rate of DNA Polymerase-a Activity on Duplex DNA by a DNA-binding Protein and a DNA-dependent ATPase of Mammalian Cells F Cobianchi, S. Riva, G. Mastromei, S. Spadari, G. Pedrali-Noy, and A. Falaschi 639
Diadenosine Tetraphosphate Triggers In Vitro DNA Replication F. Grummt 649
PART 2
Replication of Eukaryotic DNA Viruses
Protein-DNA Interactions at the Origin of Simian Virus 40 DNA Replication R. Tjian 655
Mutants of Simian Virus 40 with Base Substitutions at the Origin of DNA Replication D. Shortle and D. Nathans 663
Application of Site-directed Mutagenesis to RNA and DNA Genomes H. Weber, T. Taniguchi, W. Müller, F. Meyer, and C. Weissmann 669
Replication and Structure of Simian Virus 40 Chromosomes M. L. DePamphi/is, S. Anderson, R. Bar-Shavit, E. Collins, H. Edenberg, T Herman, B. Karas, G. Kaufmann, H. Krokan, F. Shelton, R. Su, D. Tapper, and P. M Wassarman 679
Association of Enzymes with Replicating and Nonreplicating Simian Virus 40 Chromosomes Y. Tsubota, M. A. Waqar, J. F. Burke, B. I. Milavetz, M. J. Evans, D. Kowalski, and J. A. Huberman 693
DNA Polymerases and a Single-strand-specific DNA-binding Protein Associated with Simian Virus 40 Nucleoprotein Complexes D. Otto, F. Fanning, and A. Richter 705
Studies on Simian Virus 40 Excision from Cellular Chromosomes M. Botchan, W. Topp, and J. Sambrook 709
The Structure of Replicating Adenovirus DNA Molecules: Characterization of DNA- Protein Complexes from Infected Cells T. J. Kelly, Jr. and R. L. Lechner 721
Replication of DNA in Adenovirus-infected Cells B. W. Stillman and A. J. D. Bellett 729
Detection of Covalent DNA-Protein Complexes: The Adenovirus DNA Terminal Protein Complex and HeLa DNA-Protein Complexes D. H. Coombs, A. J. Robinson, J. W. Bodnar, C. J. Jones, and G. D. Pearson 741
Adenovirus-2 DNA: In Vitro Replication and Analysis of Viral Early Proteins M. Green, M. Q. Arens, T. Yamashita, W. S. M. Wold, and K. H. Brackmann 755
Adenovirus DNA Replication in Soluble Extracts of Infected Cell Nuclei M. S. Horwitz, L. M. Kaplan, M. Abboud, J. Maritato, L. T. Chow, and T R. Broker 769
Adeno-associated Virus DNA Replication K. I. Berns, W. W. Hauswirth, K. H. Fife, and E. Lusby 781
Replication of Vaccinia DNA and Studies on the Structure of the Viral Chromo- some M. Esteban, M. Soloski, C. V. Cabrera, and J. A. Holowczak 789
Cell-free Synthesis of Herpes Simplex Virus DNA: Studies with Three Temperature- sensitive Mutants of HSV Type I B. Francke, D. R. Bone, and M. Strobel-Fidler 801
On the Structure, Functional Equivalence, and Replication of the Four Arrangements of Herpes Simplex Virus DNA B. Roizman, R.. J. Jacob, D. M. Knipe, L. S. Morse, and W. T. Ruyechan 809
Recombination in Herpes Simplex Virus: Mapping of Mutations and Analysis of Intertypic Recombinants N. M. Wilkie, A. Davison, P. Chartrand, N. D. Stow, V. G. Preston, and M. C. Timbury 827
Replication of RNA Tumor Viruses
In Vitro Studies of the Replication of Moloney Murine Leukemia Virus W. A.. Haseltine and J. M. Coffin 841
Synthesis, Structure, and Integration of the DNA of RNA Tumor Viruses H. E. Varmus, P. R. Shank, S. E. Hughes, H.-J. Kung, S. Heasley, J. Majors, P. K. Vogt, and I. M. Bishop 851
Avian RNA Tumor Virus DNA before and after Integration into the Host Genome J. M. Taylor, W. S. Mason, T. W. Hsu, J. L. Sabran, C. Yeater, G. E. Mark, A. Kaji, R.. V. Guntaka, and M. M. C. Lai 865
Production of a Discrete, Infectious, Double-stranded DNA by Reverse Transcription in Vmons of Moloney Murine Leukemia Virus D. Baltimore, E. Gilboa, E. Rothenberg, and F. Yoshimura 869
Integration and Possible Transduction by Retroviruses D. Steffen, P. Andersson, M. Goldfarb, and R.. A. Weinberg 875
Recombination Functions and Repair
Targeted and Untargeted Mutagenesis by Various Inducers of SOS Functions in Escherichia coli E. M. Witkin and I. E. Wermundsen 881
A Role for recF in Repair of UV Damage to DNA A. J. Clark, M. R. Volkert, and L. I Margossian 887
Mutagenesis- and Repair-enhancing Activities Associated with the Plasmid pKMl0l G. C. Walker 893
Molecular Events and Molecules That Lead to Induction of Prophage and SOS Functions M. Oishi, C. L. Smith, and B. Friefeld 897
Characteristics of Purified recA Protein and the Regulation of Its Synthesis In Vivo T. Ogawa, H. Wabiko, T. Tsurimoto, T. Horii, H. Masukata, and H. Ogawa 909
Activity of the Escherichia coli recA-gene Product J. W. Roberts, C. W. Roberts, N. L. Craig, and E. M. Phizicky 917
On the Fidelity of DNA Replication L. A. Loeb, L. A. Weymouth, T. A. Kunkel, K. P. Gopinathan, R. A. Beckman, and D. K. Dube 921
Characterization of a Mutator DNA Polymerase I from Salmonella typhimurium M. J. Engler and M. J. Bessman 929
Replicational Fidelity: Mechanisms of Mutation Avoidance and Mutation Fixation M. Radman, G. Villani, S. Boiteux, A. R.. Kinsella, B. W. Glickman, and S. Spadari 937
Nucleotide Excision Repair of Damaged DNA L. Grossman, S. Riazuddin, W. A.. Haseltine, and C. Lindan 947
General Recombination Branch Migration in Recombination R. C. Warner, R. A. Fishel, and F. C. Wheeler 957
DNA Recombination: In Vivo and In Vitro Studies H. Potter and D. Dressler 969
Enzymatic Cleavage of D Loops C. DasGupta, R. P. Cunningham, T. Shibata, and C M. Radding 987
Recombination in Hybrid ColEl DNAs As Analyzed by A-mediated Transduction K. Shimada, K. Umene, T. Nakamura, and Y. Takagi 991
Heteroduplex Regions in Unduplicated Bacteriophage A Recombinants M. S. Fox, C. S. Dudney, and E. J. Sodergren 999
recA-Mediated Recombination of Bacteriophage A: Structure of Recombinant and Intermediate DNA Molecules and Their Packaging In Vitro H. Ikeda and L. Kobayashi 1009
Pathways of Recombination of Bacteriophage T7 DNA In Vitro G. S. Roeder and P. D. Sadowski 1023
Recombination Intermediates Formed in the Extract from T7-infected Cells H. Ogawa, H. Araki, and Y. Tsujimoto 1033
Transduction versus "Conjuduction": Evidence for Multiple Roles for Exonuclease V in Genetic Recombination in Escherichia coli R. D. Porter, T. McLaughlin, and B. Low 1043
Mechanisms of Action of the Type-I Restriction Endonuclease, EcoB, and the recBC DNase from Escherichia coli .J. Rosamond, B. Endlich, K. M. Telander, and S. Linn 1049
A Special Type of UV-stimulated Recombination in Haemophilus influenzae E. Waldstein, J. K Setlow, and L. Santasier 1059
Chi D. K. Chattoraj, J. M. Crasemann, N Dower, D. Faulds, P. Faulds, R. E. Malone, F. W. Stahl, and M. M. Stahl 1063
Nucleotide-sequence Analysis of a Chi Site G. R. Smith, D. H. Faulds, and K. U. Sprague 1067
Plasmids Carrying oriC Can Integrate at or near the Chromosome Origin of Esch- erichia coli in the Absence of a Functional recA Product M. Masters, V. Andresdottir, and H. Wolf-Watz 1069
Early Steps in Genetic Recombination Induced by Damaged DNA: Cutting in trans in Escherichia coli and in Protein Extracts P. Howard-Flanders, E. Cassuto, and P. Ross 1073
Gene Duplication in Bacteria: Alteration of Gene Dosage by Sister-chromosome Exchanges R. P. Anderson and J. R. Roth 1083
Specialized Recombination of Phages A and Mu
Site-specific Recombination in Bacteriophage l: Structural Features of Recombining Sites A. Landy, R. H. Hoess, K. Bidwell, and W. Ross 1089
Integrative Recombination of Bacteriophage l: Requirement for Supertwisted DNA In Vivo and Characterization of mt Y. Kikuchi and H. Nash 1099
Integrative Recombination of Bacteriophage l: In Vitro Study of the Intermolecular Reaction K. Mizuuchi and M Mizuuchi 1111
The Role of l Integrase in Integration and Excision L. W. Enquist, A. Kikuchi, and R. A. Weisberg 1115
Site-specific Recombination of Bacteriophagel: The Role of Host Gene Products H. I. Miller, A. Kikuchi, H. A. Nash, R. A. Weisberg, and D. I. Friedman 1121
Regulatory Structure of the Insertion Region of Bacteriophage l L. Heffernan, M. Benedik, and A. Campbell 1127
Integration and Excision of Prophage l Mediated by the IS1 Element J. A. Shapiro and L. A. MacHattie 1135
Demonstration and Analysis of Pt Site-specific Recombination Using l-Pt Hybrid Phages Constructed In Vitro N. Steinberg 1143
Site-specific Recombination Leading to the Integration of Phages P1 and P7 R. H. Chesney, D. Vapnek, and J. R. Scott 1147
Integration of Bacteriophage Mu DNA E. Ljungquist, H. Khatoon, M. DuBow, L. Ambrosio, F. de Bruijn, and A. I. Bukhari 1151
Site-specific Recombination in Phage Mu D. Kamp, L. T. Chow, T. R. Broker, D. Kwoh, D. Zipser, and R. Kahmann 1159
Mini-Mu: An Insertion Element Derived from Temperate Phage Mu-1 M. Faelen, A. Resibois, and A. Toussaint 1169
Properties of the Recombinant Plasmid pGP1 Containing Part of the Early Region of Bacteriophage Mu M. Giphart-Gassler, T. Goosen, A. van Meeteren, C. Wijifel- man, and P. van de Putte 1179
Insertion Sequences and Transposons
Studies with IS1 and 1S4 H. Chad well, H.-J. Fritz, P. Habermann, R. Klaer, S. Kühn, and P. Starlinger 1187
DNA Sequence of IS2-7 and Generation of Mini-insertions by Replication of IS2 Sequences D. Ghosal, I. Gross, and H. Saedler 1193
Rearrangements of Genetic Material in Escherichia coli As Observed on the Bac- teriophage P1 Plasmid W. Arber, S. lada, H. Jütte, P. Caspers, J. Meyer, and C. Hänni 1197
Regional Specificity of Illegitimate Recombination Associated with the Translocatable Ampicillin-resistance Element Tn1 G. M Weinstock and D. Botstein 1209
A Transposon (Tn917) in Streptococcus faecalis That Exhibits Enhanced Transposition during Induction of Drug Resistance P. K. Tomich, F. Y. An, and D. B. Clewell 1217
Properties of an Autonomous r-Determinant from R100.1 M. Chandler, L. Silver, D. Lane, and L. Caro 1223
Translocation and Other Recombination Events Involving the Tetracycline-resistance Element Tn10 N. Kleckner and D. G. Ross 1233
Studies of the Specificity and Control of Transposition of the Tn3 Element S. N. Cohen, M. J Casadaban, J. Chou, and C.-P. D. Tu 1247 Sequence Analysis at IS1 Insertion Sites: Models for Transposition N. D. F. Grindley and D. J. Sherratt 1257
A Genetic and Sequencing Study of IS1 and Tn9 Insertions in the Escherichia coli lac Operon M. Cabs, L. Johnsrud, and J. H. Miller 1263
Nucleotide-sequence Analysis of TN3 (Ap): Implications for Insertion and Deletion H. Ohtsubo, H. Ohmori, and E. Ohtsubo 1269
Insertion Mutations Affecting Transposition of Tn3 and Replication of a ColE1 Derivative F. Heifron, M. So, and B. J. McCarthy 1279
Structure of Deletion Derivatives of a Recombinant Plasmid Containing the Trans- posable Element Tn9 in the Spacer Sequence of Xenopus laevis 5S DNA N. V. Fedoroff 1287
Recombination Genetics
Amplification of the rRNA Genes in Tetrahymena M.-C. Yao, E. Blackburn, and J. G. Gall 1293
Studies on the Amplification of Dihydrofolate Reductase Genes in Methotrexate- resistant Cultured Mouse Cells R.. T. Schimke, R. J. Kaufman, J. H. Nunberg, and S. L. Dana 1297
Properties of Yeast Transformation J. B. Hicks, A. Hinnen, and G. R. Fink 1305
The Mechanism of Conversion of Deletions and Insertions C. M. Radding 1315
Relationships between the Correction of Mismatched Bases in DNA and Mutability R.. Holliday, P. J. Pukkila, J. M. Dickson, A. Spanos, and V. Murray 1317
Meiotic Gene Conversion: A Signal of the Basic Recombination Event in Yeast S. Fogel, R.. Mortimer, K. Lusnak, and F. Tavares 1325
Aberrant 4:4 Asci, Disparity in the Direction of the Conversion, and Frequencies of Conversion in Ascobolus immersus J.-L. Rossignol, N. Paquette, and A. Nicolas 1343
Summary F. W. Stahl 1353
Symposium Participants v
Foreword xv
Introduction
Aspects of DNA Replication A. Kornberg 1
DNA Unwinding
Structural and Energetic Considerations of Wave Propagation in DNA H. M Sobell, E. D. Lozansky, and M Lessen 11
Gene-V Product of Bacteriophage fd: Structure of a DNA-unwinding Protein and Its Complexes with DNA A. McPherson, F Jumak, A. Wang, F. Kolpak, I. Molineux, and A. Rich 21
DNA: Bihelical Structure, Supercoiling, and Relaxation J. C. Wang 29
DNA Gyrase and DNA Supercoiling M. Gellert, K. Mizuuchi, M. H. O'Dea, H. Ohmori, and J. Tomizawa 35
Structure and Activities of Escherichia coli DNA Gyrase C. L. Peebles, N. P. Higgins, K. N. Kreuzer, A. Morrison, P. O. Brown, A. Sugino, and N. R. Cozzarelli 41
Studies on the Regulation and Specificity of the DNA-untwisting Enzyme J. J. Champoux, L. S. Young, and M D. Been 53
Gene-II Protein of Bacteriophage fd in Enzymatic Replication of Viral Duplex DNA K. Geider and T. F. Meyer 59
DNA Helicases B. Kuhn, M Abdel-Monem, and H. Hoffmann-Berling 63
Replication Origins
Genetic and Physical Studies on the Replication of ColE1-type Plasmids K. Back- man, M Betlach, H. W. Boyer, and S. Yanofsky 69
Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322 J. G. Sutcliffe 77
Plasmid DNA Replication: RK2- and R6K-encoded Trans-acting Factors and Their Sites of Action R.. Kolter, M. Inuzuka, D. Figurski, C. Thomas, D. Stalker, and D. R.. Helinski 91
Essential Regions for Replication of a Stringent and a Relaxed Plasmid in Escherichia coli M. L. Kahn, D. Figurski, L. Ito, and D. R.. Helinski 99
Plasmid-encoded Functions Involved in the Replication and Inheritance of Antibiotic- resistance Plasmid R6-5 K. N. Timmis, I. Andrés, P. M. Slocombe, and R.. M. Synenki 105
Use of Autonomously Replicating Mini-R6K Plasmids in the Analysis of the Replica- tion Regions of the R Plasmid R6K J. H. Crosa, L. K. Luttropp, and S. Falkow 111
Origin of Replication, oriC, of the Escherichia coli K12 Chromosome: Genetic Mapping and Minichromosome Replication K. von Meyenburg, F. G. Hansen, F. Ruse, H. E. N. Bergmans, M. Meijer, and W. Messer 121
Structural and Functional Properties of the Escherichia coli Origin of DNA Replication Y. Hirota, S. Yasuda, M. Yamada, A. Nishimura, K. Sugimoto, H. Sugisaki, A. Oka, and M Takanami 129
Origin of Replication, oriC, of the Escherichia coli K12 Chromosome: Nucleotide Sequence W. Messer, M Meijer, H. F. N. Bergmans, F. G. Hansen, K. von Meyenburg, F. Beck, and H. Schaller 139
Positive and Negative Control of Bacteriophage Lambda DNA Replication M E. Furth, J. L. Yates, and W. F. Dove 147
Dissection and Comparative Anatomy of the Origins of Replication of Lambdoid Phages D. D. Moore, K. Denniston-Thompson, K. E. Kruger, M. E. Furth, B. G. Williams, D. L. Daniels, and F R. Blattner 155
Functional Analysis of the Replicator Structure of Lambdoid Bacteriophage DNAs G. Hobom, R. Grosschede, M. Lusky, G. Scherer, E. Schwarz. and H. Kössel 165
Nucleotide Sequence of a Fragment of HeLa-cell Mitochondrial DNA Containing the Precisely Localized Origin of Replication G. Attardi, S. T. Crews, J. Nishiguchi, D. K. Ojala, and J. W. Posakony 179
Cloning and Characterization of the Replication Origin from Rat Mitochondrial DNA K. Koike, M. Kobayashi, and T. Sekiya 193
Priming and Okazaki Fragments
Structure and Metabolism of the RNA Primer in the Discontinuous Replication of Prokaryotic DNA T. Okazaki, Y. Kurosawa, T. Ogawa, T. Seki, K. Shinozaki, S. Hirose, A. Fujiyama, Y. Kohara, Y. Machida, F. Tamanoi, and T. Hozumi 203
Excision-Repair of Uracil in DNA: Its Implications for the Discontinuous Replication of DNA In Vivo and In Vitro I. R.. Lehman, B.-K. Tye, and P.-O. Nyman 221
Formation of Okazaki Pieces at the Escherichia coli Replication Fork In Vitro K. R.. Thomas, P. Manlapaz-Ramos, R.. Lundquist, and B. M. Olivera 231
Uracil Incorporation into Nascent DNA of Bacillus subtilis and Escherichia coli F Tamanoi, Y. Machida, and T. Okazaki 239
Evidence for Many Nonnascent DNA Molecules the Size of Okazaki Fragments in Escherichia coli and 3T3 Cells: Very Few Have 5¢-Terminal Ribonucleotides D. T. Denhardt, J. Kowaiski, and C. Miyamoto 243
Replication of Mouse L-cell Mitochondrial DNA D. Bogenhagen, A. M. Gillum, P. A. Martens, and D. A. Clayton 253
Studies on Early Intermediates in the Synthesis of DNA in Animal Cells B. Y Tseng, R.. H. Grafstrom, D. Revie, W. Oertel, and M. Goulian 263
Synthesis and Function of Polyoma Initiator RNA P. Reichard and R.. Eliasson 271
Characterization of the 5¢ Ends of Nascent DNA Chains R.. Werner, D. W. Siegmann, A. T. Diaz, and H. Jering 279
Replication of Small Phages and Plasmids
Enzymatic Conversion of Single-stranded f Xl74 and G4 Circles to Duplex Forms: Discontinuous Replication R.. R.. Meyer, J. Shlomai, J. Kobori, D. L. Bates, L. Rowen, R. McMacken, K. Ueda, and A. Kornberg 289
Enzymatic Replication of f X174 Duplex Circles: Continuous Synthesis S. Eisenberg, J. F. Scott. and A. Kornberg 295
DNA Replication Proteins of Escherichia coli and Phage l S. H. Wickner 303
Replication of f X174 DNA: In Vitro Synthesis of f X RFI DNA and Circular, Single-stranded DNA C. Sumida-Yasumoto, J.-E. Ikeda, E. Benz, K. J. Marians, R.. Vicuna, S. Sugrue, S. L. Zipursky, and J. Hurwitz 311
Cleavage Site of f X174 Gene-A Protein in f X and G4 RFI DNA A. D. M. van Mansfeld, S. A. Langeveld, P. J. Weisbeek, P. D. Baas, G. A. van Arkel, and H. S. Jansz 331
The rep Protein of Escherichia coli: Interaction with DNA and Other Proteins M. Duguet. G. Yarranton, and M. Gefter 335
DNA-dependent ATPases from Escherichia coli K12 F. Richet, 1. Moreau, and M Kohiyama 345
Single-stranded Phage Replication: Positive- and Negative-strand DNA Synthesis J. Sims, K. Koths, and D. Dressier 349 G4 DNA Replication In Vivo and the Origins of Its Strand Synthesis G. N. Godson 367
Nucleotide Sequence of the Origin for Bacteriophage M13 DNA Replication S. V. Suggs and D. S. Ray 379
DNA Replication of Bacteriophage fi In Vivo K. Horiuchi, J. V. Ravetch, and D. Zinder 389
The Intergenic Region and the Origins for Filamentous Phage DNA Replication H. Schaller 401
Initiation of Replication of Plasmid CoIEl DNA by RNA Polymerase, Ribonuclease H, and DNA Polymerase I T. Itoh and J. Tomizawa 409
DNA Replication Control: Studies of Plasmid Ri P. Gustafsson, H. Dreisig, S. Molin, K. Nordström, and B. Uhlin 419
Replication of Large Phages and Bacteria
Replication of Bacteriophage T7 DNA by Purified Proteins C. C. Richardson, L. J. Romano, R.. Kolodner, J. E. LeClerc, F. Tamanoi, M. J. Engler, F. B. Dean, and D. S. Richardson 427
Cloning of the T7 Genome in Escherichia coli: Use of Recombination between Cloned Sequences and Bacteriophage T7 to Identify Genes Involved in Recombination and a Clone Containing the Origin of T7 DNA Replication J. L. Campbell, F. Tamanoi, C. C. Richardson, and F. W. Studier 441
Bacteriophage T7 DNA Primase: A Multifunctional Enzyme Involved in DNA Replication G. Hillenbrand, G. Morelli, F. Lanka, and F. Scherzinger 449
Cross-reactivation and Partial Replication of UV-irradiated T7+ DNA K. B. Burck, D. M. Taylor, H W Smith, and R.. C. Miller, Jr. Probing DNA Replication Mechanisms with the T4 Bacteriophage In Vitro System C. C. Liu, R. L. Burke, U Hibner, J. Barry, and B. Alberts 469
DNA Replication by Bacteriophage T4 Proteins: Role of the DNA-delay Gene 61 in the Chain-initiation Reaction L. L. Silver and N. G. Nossal 489
Positive Regulation of T-even-phage DNA Replication by the DNA-delay Protein of Gene 39 W M Huang 495
In Vivo Interactions of Genes and Proteins in DNA Replication and Recombination of Phage T4 G. Mosig, A. Luder, G. Garcia, R.. Dannenberg, and S. Bock Late Replication and Recombination in the Vegetative Pool of T4 N. Hutchinson, T. Kazic, S. J. Lee, C. Rayssiguier, B. S. Emanuel, and W. Kozinski 517
f 29 DNA-Protein Complex and the Structure of Replicating DNA Molecules J. Ito, N. F. Harding, and K. Saigo 525
recA-Dependent DNA Replication in the Absence of Protein Synthesis: Characteris- tics of a Dominant Lethal Replication Mutation, dnaT, and Requirement for recA+ Function K. G. Lark and C. A. Lark 537
A dnaB-Analog DNA-replication Protein of Phage P1 H Schuster, F. Lanka, C Edelbluth, B. Geschke, M. Mikolajczyk, M Schlicht, and D. Touati-Schwartz 551
The Escherichia co/i dnaB Protein Is Required for Initiation of Chromosomal DNA Replication F. K. Fujimura, J. W. Zyskind, and D. W Smith 559
Chromosome Replication in Escherichia coli Is Inhibited in the Terminus Region Near the rac Locus P. L. Kuempel and S. A. Duerr 563
Organization of the Replication-origin Region of the Bacillus subtilis Chromosome H. Yoshikawa, K. Yamaguchi, M. Seiki, N. Ogasawara, and H. Toyoda 569
Replication of Eukaryotic DNA
Studies on the Prereplicative Phase of the Cell Cycle in Saccharomyces cerevisiae D. R. W. Edwards, J. B. Taylor, W. F Wakeling, F. Z. Watts, and I. R. Johnston 577
A Stimulatory Factor for Yeast DNA Polymerase L. M. S. Chang, K. Lurie, and P. Plevani 587
Eukaryotic DNA Replication: The First Steps toward a Multienzyme System from Xenopus laevis R. M Benbow, C. B. Breaux, H. Joenje, M. R. Krauss, R. W. Lennox, F. M. Nelson, N. S. Wang, and S. H. White 597
Two Cell-free Systems Constructed from Xenopus laevis Oocytes: Generalized Genetic Recombination; DNA Supercoiling G. P. Tocchini-Valentini, M. I. Baldi, G. Carrara, D. G. Attardi, and F. Mattoccia 603
Structural and Enzymological Properties of Human DNA Polymerases a and b D. Korn, P. A. Fisher, J. Battey, and T. S.-F. Wang 613
Functions of DNA Polymerases a, b, and g in Neurons during Development U. Hübscher, C. C. Kuenzle, W. Limacher, P. Scherrer, and S. Spadari 625
Studies on the Interaction of tRNA and Avian Myeloblastosis DNA Polymerase A. Araya and S. Litvak 631
Enhancement of the Rate of DNA Polymerase-a Activity on Duplex DNA by a DNA-binding Protein and a DNA-dependent ATPase of Mammalian Cells F Cobianchi, S. Riva, G. Mastromei, S. Spadari, G. Pedrali-Noy, and A. Falaschi 639
Diadenosine Tetraphosphate Triggers In Vitro DNA Replication F. Grummt 649
PART 2
Replication of Eukaryotic DNA Viruses
Protein-DNA Interactions at the Origin of Simian Virus 40 DNA Replication R. Tjian 655
Mutants of Simian Virus 40 with Base Substitutions at the Origin of DNA Replication D. Shortle and D. Nathans 663
Application of Site-directed Mutagenesis to RNA and DNA Genomes H. Weber, T. Taniguchi, W. Müller, F. Meyer, and C. Weissmann 669
Replication and Structure of Simian Virus 40 Chromosomes M. L. DePamphi/is, S. Anderson, R. Bar-Shavit, E. Collins, H. Edenberg, T Herman, B. Karas, G. Kaufmann, H. Krokan, F. Shelton, R. Su, D. Tapper, and P. M Wassarman 679
Association of Enzymes with Replicating and Nonreplicating Simian Virus 40 Chromosomes Y. Tsubota, M. A. Waqar, J. F. Burke, B. I. Milavetz, M. J. Evans, D. Kowalski, and J. A. Huberman 693
DNA Polymerases and a Single-strand-specific DNA-binding Protein Associated with Simian Virus 40 Nucleoprotein Complexes D. Otto, F. Fanning, and A. Richter 705
Studies on Simian Virus 40 Excision from Cellular Chromosomes M. Botchan, W. Topp, and J. Sambrook 709
The Structure of Replicating Adenovirus DNA Molecules: Characterization of DNA- Protein Complexes from Infected Cells T. J. Kelly, Jr. and R. L. Lechner 721
Replication of DNA in Adenovirus-infected Cells B. W. Stillman and A. J. D. Bellett 729
Detection of Covalent DNA-Protein Complexes: The Adenovirus DNA Terminal Protein Complex and HeLa DNA-Protein Complexes D. H. Coombs, A. J. Robinson, J. W. Bodnar, C. J. Jones, and G. D. Pearson 741
Adenovirus-2 DNA: In Vitro Replication and Analysis of Viral Early Proteins M. Green, M. Q. Arens, T. Yamashita, W. S. M. Wold, and K. H. Brackmann 755
Adenovirus DNA Replication in Soluble Extracts of Infected Cell Nuclei M. S. Horwitz, L. M. Kaplan, M. Abboud, J. Maritato, L. T. Chow, and T R. Broker 769
Adeno-associated Virus DNA Replication K. I. Berns, W. W. Hauswirth, K. H. Fife, and E. Lusby 781
Replication of Vaccinia DNA and Studies on the Structure of the Viral Chromo- some M. Esteban, M. Soloski, C. V. Cabrera, and J. A. Holowczak 789
Cell-free Synthesis of Herpes Simplex Virus DNA: Studies with Three Temperature- sensitive Mutants of HSV Type I B. Francke, D. R. Bone, and M. Strobel-Fidler 801
On the Structure, Functional Equivalence, and Replication of the Four Arrangements of Herpes Simplex Virus DNA B. Roizman, R.. J. Jacob, D. M. Knipe, L. S. Morse, and W. T. Ruyechan 809
Recombination in Herpes Simplex Virus: Mapping of Mutations and Analysis of Intertypic Recombinants N. M. Wilkie, A. Davison, P. Chartrand, N. D. Stow, V. G. Preston, and M. C. Timbury 827
Replication of RNA Tumor Viruses
In Vitro Studies of the Replication of Moloney Murine Leukemia Virus W. A.. Haseltine and J. M. Coffin 841
Synthesis, Structure, and Integration of the DNA of RNA Tumor Viruses H. E. Varmus, P. R. Shank, S. E. Hughes, H.-J. Kung, S. Heasley, J. Majors, P. K. Vogt, and I. M. Bishop 851
Avian RNA Tumor Virus DNA before and after Integration into the Host Genome J. M. Taylor, W. S. Mason, T. W. Hsu, J. L. Sabran, C. Yeater, G. E. Mark, A. Kaji, R.. V. Guntaka, and M. M. C. Lai 865
Production of a Discrete, Infectious, Double-stranded DNA by Reverse Transcription in Vmons of Moloney Murine Leukemia Virus D. Baltimore, E. Gilboa, E. Rothenberg, and F. Yoshimura 869
Integration and Possible Transduction by Retroviruses D. Steffen, P. Andersson, M. Goldfarb, and R.. A. Weinberg 875
Recombination Functions and Repair
Targeted and Untargeted Mutagenesis by Various Inducers of SOS Functions in Escherichia coli E. M. Witkin and I. E. Wermundsen 881
A Role for recF in Repair of UV Damage to DNA A. J. Clark, M. R. Volkert, and L. I Margossian 887
Mutagenesis- and Repair-enhancing Activities Associated with the Plasmid pKMl0l G. C. Walker 893
Molecular Events and Molecules That Lead to Induction of Prophage and SOS Functions M. Oishi, C. L. Smith, and B. Friefeld 897
Characteristics of Purified recA Protein and the Regulation of Its Synthesis In Vivo T. Ogawa, H. Wabiko, T. Tsurimoto, T. Horii, H. Masukata, and H. Ogawa 909
Activity of the Escherichia coli recA-gene Product J. W. Roberts, C. W. Roberts, N. L. Craig, and E. M. Phizicky 917
On the Fidelity of DNA Replication L. A. Loeb, L. A. Weymouth, T. A. Kunkel, K. P. Gopinathan, R. A. Beckman, and D. K. Dube 921
Characterization of a Mutator DNA Polymerase I from Salmonella typhimurium M. J. Engler and M. J. Bessman 929
Replicational Fidelity: Mechanisms of Mutation Avoidance and Mutation Fixation M. Radman, G. Villani, S. Boiteux, A. R.. Kinsella, B. W. Glickman, and S. Spadari 937
Nucleotide Excision Repair of Damaged DNA L. Grossman, S. Riazuddin, W. A.. Haseltine, and C. Lindan 947
General Recombination Branch Migration in Recombination R. C. Warner, R. A. Fishel, and F. C. Wheeler 957
DNA Recombination: In Vivo and In Vitro Studies H. Potter and D. Dressler 969
Enzymatic Cleavage of D Loops C. DasGupta, R. P. Cunningham, T. Shibata, and C M. Radding 987
Recombination in Hybrid ColEl DNAs As Analyzed by A-mediated Transduction K. Shimada, K. Umene, T. Nakamura, and Y. Takagi 991
Heteroduplex Regions in Unduplicated Bacteriophage A Recombinants M. S. Fox, C. S. Dudney, and E. J. Sodergren 999
recA-Mediated Recombination of Bacteriophage A: Structure of Recombinant and Intermediate DNA Molecules and Their Packaging In Vitro H. Ikeda and L. Kobayashi 1009
Pathways of Recombination of Bacteriophage T7 DNA In Vitro G. S. Roeder and P. D. Sadowski 1023
Recombination Intermediates Formed in the Extract from T7-infected Cells H. Ogawa, H. Araki, and Y. Tsujimoto 1033
Transduction versus "Conjuduction": Evidence for Multiple Roles for Exonuclease V in Genetic Recombination in Escherichia coli R. D. Porter, T. McLaughlin, and B. Low 1043
Mechanisms of Action of the Type-I Restriction Endonuclease, EcoB, and the recBC DNase from Escherichia coli .J. Rosamond, B. Endlich, K. M. Telander, and S. Linn 1049
A Special Type of UV-stimulated Recombination in Haemophilus influenzae E. Waldstein, J. K Setlow, and L. Santasier 1059
Chi D. K. Chattoraj, J. M. Crasemann, N Dower, D. Faulds, P. Faulds, R. E. Malone, F. W. Stahl, and M. M. Stahl 1063
Nucleotide-sequence Analysis of a Chi Site G. R. Smith, D. H. Faulds, and K. U. Sprague 1067
Plasmids Carrying oriC Can Integrate at or near the Chromosome Origin of Esch- erichia coli in the Absence of a Functional recA Product M. Masters, V. Andresdottir, and H. Wolf-Watz 1069
Early Steps in Genetic Recombination Induced by Damaged DNA: Cutting in trans in Escherichia coli and in Protein Extracts P. Howard-Flanders, E. Cassuto, and P. Ross 1073
Gene Duplication in Bacteria: Alteration of Gene Dosage by Sister-chromosome Exchanges R. P. Anderson and J. R. Roth 1083
Specialized Recombination of Phages A and Mu
Site-specific Recombination in Bacteriophage l: Structural Features of Recombining Sites A. Landy, R. H. Hoess, K. Bidwell, and W. Ross 1089
Integrative Recombination of Bacteriophage l: Requirement for Supertwisted DNA In Vivo and Characterization of mt Y. Kikuchi and H. Nash 1099
Integrative Recombination of Bacteriophage l: In Vitro Study of the Intermolecular Reaction K. Mizuuchi and M Mizuuchi 1111
The Role of l Integrase in Integration and Excision L. W. Enquist, A. Kikuchi, and R. A. Weisberg 1115
Site-specific Recombination of Bacteriophagel: The Role of Host Gene Products H. I. Miller, A. Kikuchi, H. A. Nash, R. A. Weisberg, and D. I. Friedman 1121
Regulatory Structure of the Insertion Region of Bacteriophage l L. Heffernan, M. Benedik, and A. Campbell 1127
Integration and Excision of Prophage l Mediated by the IS1 Element J. A. Shapiro and L. A. MacHattie 1135
Demonstration and Analysis of Pt Site-specific Recombination Using l-Pt Hybrid Phages Constructed In Vitro N. Steinberg 1143
Site-specific Recombination Leading to the Integration of Phages P1 and P7 R. H. Chesney, D. Vapnek, and J. R. Scott 1147
Integration of Bacteriophage Mu DNA E. Ljungquist, H. Khatoon, M. DuBow, L. Ambrosio, F. de Bruijn, and A. I. Bukhari 1151
Site-specific Recombination in Phage Mu D. Kamp, L. T. Chow, T. R. Broker, D. Kwoh, D. Zipser, and R. Kahmann 1159
Mini-Mu: An Insertion Element Derived from Temperate Phage Mu-1 M. Faelen, A. Resibois, and A. Toussaint 1169
Properties of the Recombinant Plasmid pGP1 Containing Part of the Early Region of Bacteriophage Mu M. Giphart-Gassler, T. Goosen, A. van Meeteren, C. Wijifel- man, and P. van de Putte 1179
Insertion Sequences and Transposons
Studies with IS1 and 1S4 H. Chad well, H.-J. Fritz, P. Habermann, R. Klaer, S. Kühn, and P. Starlinger 1187
DNA Sequence of IS2-7 and Generation of Mini-insertions by Replication of IS2 Sequences D. Ghosal, I. Gross, and H. Saedler 1193
Rearrangements of Genetic Material in Escherichia coli As Observed on the Bac- teriophage P1 Plasmid W. Arber, S. lada, H. Jütte, P. Caspers, J. Meyer, and C. Hänni 1197
Regional Specificity of Illegitimate Recombination Associated with the Translocatable Ampicillin-resistance Element Tn1 G. M Weinstock and D. Botstein 1209
A Transposon (Tn917) in Streptococcus faecalis That Exhibits Enhanced Transposition during Induction of Drug Resistance P. K. Tomich, F. Y. An, and D. B. Clewell 1217
Properties of an Autonomous r-Determinant from R100.1 M. Chandler, L. Silver, D. Lane, and L. Caro 1223
Translocation and Other Recombination Events Involving the Tetracycline-resistance Element Tn10 N. Kleckner and D. G. Ross 1233
Studies of the Specificity and Control of Transposition of the Tn3 Element S. N. Cohen, M. J Casadaban, J. Chou, and C.-P. D. Tu 1247 Sequence Analysis at IS1 Insertion Sites: Models for Transposition N. D. F. Grindley and D. J. Sherratt 1257
A Genetic and Sequencing Study of IS1 and Tn9 Insertions in the Escherichia coli lac Operon M. Cabs, L. Johnsrud, and J. H. Miller 1263
Nucleotide-sequence Analysis of TN3 (Ap): Implications for Insertion and Deletion H. Ohtsubo, H. Ohmori, and E. Ohtsubo 1269
Insertion Mutations Affecting Transposition of Tn3 and Replication of a ColE1 Derivative F. Heifron, M. So, and B. J. McCarthy 1279
Structure of Deletion Derivatives of a Recombinant Plasmid Containing the Trans- posable Element Tn9 in the Spacer Sequence of Xenopus laevis 5S DNA N. V. Fedoroff 1287
Recombination Genetics
Amplification of the rRNA Genes in Tetrahymena M.-C. Yao, E. Blackburn, and J. G. Gall 1293
Studies on the Amplification of Dihydrofolate Reductase Genes in Methotrexate- resistant Cultured Mouse Cells R.. T. Schimke, R. J. Kaufman, J. H. Nunberg, and S. L. Dana 1297
Properties of Yeast Transformation J. B. Hicks, A. Hinnen, and G. R. Fink 1305
The Mechanism of Conversion of Deletions and Insertions C. M. Radding 1315
Relationships between the Correction of Mismatched Bases in DNA and Mutability R.. Holliday, P. J. Pukkila, J. M. Dickson, A. Spanos, and V. Murray 1317
Meiotic Gene Conversion: A Signal of the Basic Recombination Event in Yeast S. Fogel, R.. Mortimer, K. Lusnak, and F. Tavares 1325
Aberrant 4:4 Asci, Disparity in the Direction of the Conversion, and Frequencies of Conversion in Ascobolus immersus J.-L. Rossignol, N. Paquette, and A. Nicolas 1343
Summary F. W. Stahl 1353
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