 |
 XLII: Chromatin 1977 |
 |
|
|
From left: Dr. Pierre Chambon, Dr. Markus Noll, Dr. Francis Crick  SYMPOSIUM SYNOPSIS LIST OF PARTICIPANTS CONTENTS OF SYMPOSIUM VOLUME PHOTOGRAPHS PUBLISHED VOLUME  |
Chromatin
Symposium Volume XLII Contents
PART 1
Symposium Participants v
Foreword xv
Nucleosome Structure I: Crystallographic Analysis
X-ray and Electron Microscope Analyses of Crystals of Nucleosome Cores J. T. Finch and A. Klug 1
Scattering Studies of Chromatin Subunits J. F. Pardon, R. I. Cotter, D. M. J. Lilley, D. L. Worcester, A. M. Campbell, J. C. Wooley, and B. M. Richards 11
DNA Structure in Chromatin and in Solution Studied by Electron Microscopy and Neutron and X-ray Scattering S. Bram, S. Kouprach, and P. Baudy 23
Nucleosome Structure II: Conformational Studies
Conformational States of Chromatin M. Zama, P.N. Bryan, P. F. Harrington, A. L. Olins, and D. F. Olins 31
Ultrastructure of Chromatin Subunits during Unfolding, Histone Depletion, and Reconstruction C. L. F. Woodcock and L-L. Y. Frado 43
Nucleosome Structure, DNA Folding, and Gene Activity R. D. Camerini- Otero, B. Sollner- Webb, R.. H. Simon, P. Williamson, M. Zasloff and G. Felsenfeld 57
Internal Structure of the Nucleosome: DNA Folding in the Conserved 140-Base- pair Core Particle M. Noll 77
Conformational Flexibility in DNA Structure as Revealed by Structural Studies of Drug Intercalation and Its Broader Implications in Understanding the Organization of DNA in Chromatin H. M. Sobell, B. S. Reddy, K. K. Bhan- dary, S. C. Jain, T. D. Sakore, and T. P. Seshadri 87
Relation of Nucleosomes to DNA Sequences A. Prunell and R. D. Kornberg 103
Domains in Chromatin Structure T. 1gó-Kemenes and H G. Zachau 109
Nucleosome Structure III: The Core
The Nucleosome Core Protein J. O. Thomas and P. J. C. Butler 119
Histone-DNA Interactions in Chromatin Core Particles R. T. Simpson, J. P. Whitlock, Jr., M. Bina-Stein, and A. Stein 127
Characterization of DNase-I Cleavage Sites in the Nucleosome L. C. Lutter 137
The Structure of Nucleosomes: The Arrangement of Histones in the DNA Grooves and Along the DNA Chain A. D. Mirzabekov, V. V. Shick, A.. V. Belyavsky, V. L. Karpov, and S. G. Bavykin 149
Evolutionary Conservation of Histone-Histone Binding Sites: Evidence from In- terkingdom Complex Formation S. Spiker and I. Isenberg 157
Nucleohistone Assembly: Sequential Binding of Histone H3-H4 Tetramer and Histone H2A-H2B Dimer to DNA A. Ruiz-Carrillo and J. L. Jorcano 165
Chromatin Assembly and Transcription in Eggs and Qocytes of Xenopus lae- viz R.. A. Lasky, B. M. Honda, A.. D. Mills, N. R. Morris, A. H. Wyllie, J. E. Mertz, F. M. De Robertis, and J. B. Gurdon 171
Chromatin Structures of Main-band and Satellite DNAs in Drosophila melano- gaster Nuclei as Probed by Photochemical Cross-linking of DNA with Trioxsalen C.-K. J. Shen and J. F. Hearst 179
Chromatin Structure in Living Cells T. R. Cech, D. Potter, and M. L. Pardue Dynamics of Nucleosome Structure Studied by Fluorescence A. F. Dieterich, R.. Axel, and C. R.. Cantor 199
Electric Dichroism Studies of the Size and Shape of Nucleosomal Particles L. Klevan, M. Hogan, N. Dattagupta, and D. M. Crothers 207
Nucleosome Structure IV: H1 Interactions
The Multifunctional Role of Histone H1, Probed with the SV40 Mini- chromosome J. D. Griffith and G. Christiansen 215
Biochemical and Ultrastructural Analysis of SV40 Chromatin W. Keller, U. Müller, I. Eicken, I. Wendel, and H. Zentgraf 227
Histone Hi Involvement in the Structure of the Chromosome Fiber M. Renz, P. Nehls, and J. Hozie 245
H1 Histone and the Condensation of Chromatin and DNA R.. D. Cole, G. M. Lawson, and M. W. Hsiang 253
The Role of H1 in the Structure of Chromatin J. Gaubatz, R. Hardison, J. Murphy, M. E. Eichner, and R. Chalkley 265
Satellite DNA, Hi Histone, and Heterochromatin in Drosophila virus M. Blu- menfeld, J. W. Orf B. J. Sina, R. A. Kreber, M. A.. Callahan, and L. A. Snyder 273
Nucleosomes, Histone Interactions, and the Role of Histones H3 and H4 E. M. Bradbury, T. Moss, H. Hayashi, R. P. Hjelm, P. Suau, R. M. Stephens, J. P. Baldwin, and C. Crane-Robinson 277
Nucleosome Structure I: All Four Histones, H2A, H2B, H3, and H4, Are Required to Form a Nucleosome, but an H3-H4 Subnucleosomal Particle Is Formed with H3-H4 Alone P. Oudet, J. E. Germond, M. Sures, D. Gallwitz, M. Bel- lard, and P. Chambon 287
Nucleosome Structure II: Structure of the SV40 Minichromosome and Electron Microscopic Evidence for Reversible Transitions of the Nucleosome Struc- ture P. Oudet, C Spadafora, and P. Chambon 301
Chromosome Coiling and Assembly
Molecular Architecture of the Chromatin Fiber A. Worcel 313
v Bodies Are Close-packed in Chromatin Fibers A. L. Olins 325
A Direct Approach to the Structure of Eukaryotic Chromosomes J. Sedat and L. Manuelidis 331
Metaphase Chromosome Structure: The Role of Nonhistone Proteins U. K. Laemmli S. M. Cheng, K. W. Adolph, J. R.. Paulson, J. A. Brown, and W. R.. Baumbach 351
Metaphase Chromosome Architecture W. Wray, M. Mace, Jr., V. Daskal, and F. Stubblefield 361
Higher-order Structure of Mitotic Chromosomes A. Leth Bak and J. Zeuthen 367
Immunological Probes for Chromatin Structure M. Bustin, P. D. Kurth, E. N. Moudrianakis, D. Goldblatt, R. Sperling, and W. B. Rizzo 379
Functional Organization of Polytene Chromosomes M. Jamrich, A. L. Greenleaf F. A. Bautz and E. K. F. Bautz 389
Immunochemical Studies on the 5-Methylcytosine Content of African Green Mon- key Satellite DNA Z. F. Kahana, O. J. Miller, and B. F. Erlanger 397
The Generation and Propagation of Variegated Chromosome Structures H. Weintraub, S. J. Flint, I. M. Leffak, M. Groudine, and R.. M. Grainger 401
Assembly of SV40 and Polyoma Minichromosomes during Replication C. Cré- misi, A. Chestier, and M. Yani 409
Segregation of Some Chromosomal Proteins during Cell Replication E. F. Freed- lender, L. Taichman, and O. Smithies 417
Histone Changes during Chromatin Remodeling in Embryogenesis K. M. New- rock, C. R. Alfageme, R.. V. Nardi, and L. H. Cohen 421
Persistence of Nucleosomes on DNA during Chromatin Replication R. L. Seale 433
Simple Systems
Localization of the HU Protein on the Escherichia coli Nucleoid J. Rouvière- Yani 439
The Structure of Genes, Intergenic Sequences, and mRNA from SV40 Virus B. Thimmappaya, V. B. Reddy, R. Dhar, M. Celma, K. N. Subramanian, B. S. Zain, J. Pan, and S. M. Weissman 449
On the Structure of Eukaryotic, Prokaryotic, and Viral Chromatin A. J. Var- shavsky, V. V. Bakayev, S. A. Nedospasov, and G. P. Georgiev 457
Histone Organization in Chromatin: Comparisons of Nucleosomes and Subnucleo- somal Particles from Erythrocyte, Myeloma, and Yeast Chromatin R. L. Rill and D. A. Nelson 475
The Gene-size DNA Molecules in Oxytricha R. M. Lawn, J. M. Heumann, G. Herrick, and D. M. Prescott 483
Histones and Chromatin Structure in Tetrahymena Macro- and Micronuclei M. A. Gorovsky, C. Glover, C. A. Johmann, J. B. Keevert, D. J. Mathis, and M. Samuelson 493
Structural Organization and Control of the Ribosomal Genes in Physarum V. G. Allfrey, E. M. Johnson, I. Y.-C. Sun, V. C. Littau, H. R.. Matthews, and E. M. Bradbury 505
Transcription I: Primary Products
The Definition of Transcription Units for mRNA eli B. Darnell, R. Evans, N. Fraser, S. Goldberg J. Nevins, M. Salditt-Georgieff H. Schwartz, J. Weber, and B. Ziff 515
Spliced Segments at the 5' Termini of Adenovirus-2 Late mRNA: A Role for Heterogeneous Nuclear RNA in Mammalian Cells S. M. Berget, A. J. Berk, T. Harrison, and P. A. Sharp 523
Adenovirus-2 Messengers - An Example of Baroque Molecular Architecture T. R.. Broker, L. T. Chow, A.. R. Dunn, R. E. Gelinas, J. A. Hassell, D. F. Klessig, J. B. Lewis, R. J. Roberts, and B. S. Zain 531
Displacement Loops in Adenovirus DNA-RNA Hybrids H. Westphal and S.-P. Lai 555
Splicing of SV40 mRNAs: A Novel Mechanism for the Regulation of Gene Expres- sion in Animal Cells Y. Aloni, S. Bratosin, R. Dhar, O. Laub, M. Horowitz, and G. Khoury 559
A Novel Sequence Arrangement of SV40 Late RNA M.-T. Hsu and J. Ford 571
Faithful Gene Transcription by Eukaryotic RNA Polymerases in Reconstructed Systems C. S. Parker, J. A. Jaehning and R. G. Roeder 577
Studies on Gene Transcription In Vitro by Analysis of the Primary Tran- scripts R. C. C. Huang, M. M. Smith, and A.. E. Reeve 589
Properties of Transcriptionally Active Chromatin J. Paul, E. J. Zollner, R. S. Gilmour, and G. D. Birnie 597
PART 2
Transcription II: Regulated Systems
Regulation of Gene Expression in Chick Oviduct B. W. O'Malley, M.-J. Tsau, S. Y. Tsai and H. C. Towle 605
Hormonal Control of Egg White Protein Messenger RNA Synthesis in the Chicken Oviduct G. Shütz, M. C. Nguyen-Huu, K Giesecke, N. B. Hynes, B. Groner, T. Wurtz, and A. E. Sippel 617
Mammary Tumor Virus DNA: A Glucocorticoid-responsive Transposable Ele- ment K. R.. Yamamoto, M. R. Stallcup, J. Ring, and G. M. Ringold 625
Regulation of Gene Expression in the Chick Oviduct by Steroid Hormones R. D. Palmiter, B. R. Mulvihill, G. S. McKnight, and A. W. Senear 639
Amplification of Folate Reductase Genes in Methotrexate-resistant Cultured Mouse Cells R. T. Schimke, F. W. Alt, R. E. Kellems, R. Kaufman, and J. B. Bertino 649
Hormonal Modulation of a 2u Globulin mRNA: Sequence Measurements Using a Specific cDNA Probe P. Feigelson and D. T. Kurtz 659
The Nucleolus, a Model for Analysis of Chromatin Controls H. Busch, N. R. Ballal, R. K. Busch, Y. C. Choi, F. Davis, I. L. Goldknopf, S.-I. Matsui, M. S. Rao, and L. I. Rothblum 665
Stimulation of Ribosomal RNA Synthesis in Isolated Nuclei and Nucleoli by Partially Purified Preparations of SV40 T Antigen R. Baserga,, T. Ide, and S. Whelly 685
Purification of a Protein from Unfertilized Eggs of Drosophila with Specific Affin- ity for a Defined DNA Sequence and the Cloning of This DNA Sequence in Bacterial Plasmids H. Weideli, P. Schedl, S. Artivanis-Tsakonas, R.. Steward, R. Yuan, and W. J. Gehring 693
Transcriptionaily Active Chromatin
The Structure of the Transcriptionally Active Ovalbumin Genes in Chroma tin A.. Garel and R.. Axel 701
Structure of Xenopus Ribosomal Gene Chromatin during Changes in Genomic Transcription Rates R. Reeves 709
Modulation of Ribosomal RNA Synthesis in Oncopeltus fasciatus: An electron Microscopic Study of the Relationship between Changes in Chromatin Struc- ture and Transcriptional Activity V. E. Foe 723
Electron Microscopic Analysis of Chromosome Metabolism in the Drosophila me- lanogaster Embryo S. L. McKnight, M. Bustin, and O. L. Miller, Jr 741
Morphology of Transcriptionally Active Chromatin W. W. Franke U Scheer, M. Trendelenburg, H. Zentgraf and H. Spring 755
Structure of rDNA-containing Chromatin of Tetrahymena pyriformis Analyzed by Nuclease Digestion D. J. Mathis and M. A. Gorovsky 773
Nucleosome Structure III: The Structure and Transcriptional Activity of the Chromatin Containing the Ovalbumin and Globin Genes in Chick Oviduct Nuclei M. Bellard, F. Gannon, and P. Chambon 779
Structure and Function of the Low-salt Extractable Chromosomal Proteins. Pref- erential Association of Trout Testis Proteins H6 and HMG-T with Chromatin Regions Selectively Sensitive to Nucleases B. Levy W., N. C. W. Wong D.C. Watson, E.H. Peters, and G.H. Dixon 793
Transcription of Polytene Chromosomes and of the Mitochondrial Genome in Drosophila melanogaster J. J. Bonner, M. Berninger, and M. L. Pardue 803
Modified Histones in HeLa and Friend Erythroleukemia Cells Treated with n- Butyrate M. G. Riggs, R.. G. Whittaker, J. R. Neumann, and V. M. Ingram 815
The Effect of Heat Shock on Gene Expression in Drosophila melanogaster M.-E.. Mirault, M. Goldschmidt-Clermont, L. Moran, A. P. Arrigo, and A. Tissières 819
Correlation of Structural Changes in Chromatin with Transcription in the Drosophila Heat-shock Response H. Biessmann, S. Wadsworth, B. Levy W., and B. J. McCarthy 829
An In Vitro Assay for the Specific Induction and Regression of Puffs in Iso- lated Polytene Nuclei of Drosophila melanogaster J. L. Compton and J. J. Bonner 835
Distribution Patterns of Drosophila Nonhistone Chromosomal Proteins S. C. R.. Elgin, L. A. Serunian, and L. M. Silver 839
The Expressed Portion of Eukaryotic Chromatin J. Bonner, R. B. Wallace, T. D. Sargent, R. F. Murphy, and S. K. Dube 851
"Native" Salivary Chromosomes of Drosophila melanogaster R. J. Hill and F. Watt 859
The Size and Chromosomal Location of the 755
RNA Transcription Unit in Balbiani Ring 2 B. Daneholt, S. T. Case, J. Derksen, M. M. Lamb, L. Nelson, and L. Wieslander 867
Fate of Balbiani-ring RNA In Vivo J.-E. Edström,, E. Ericson, S. Lindgren, U. Lönn, and L. Rydlande 877
The Packaging Proteins of Core hnRNP Particles and the Maintenance of Prolif- erative Cell States W. M. LeStourgeon, A. L. Beyer, M. E. Christensen, B. W. Walker, S. M. Poupore, and L. P. Daniels 885
Substructure of Nuclear Ribonucleoprotein Complexes T. Martin, P. Billings, J. Pullman, B. Stevens, and A. Kinniburgh 899
Free Informofers and Reconstitution of 30S RNP V. V. Kulguskin, E. M. Lukani- din, and G. P. Georgiev 911
Cloned Eukaryotic Genes I
The Cloning of Mouse Globin and Surrounding Gene Sequences in Bacterio- phage l P. Leder, S. M. Tilghman, D.C. Tiemeier, F. I. Polsky, J. G. Seidman, M. H. Edgell, L. W. Enquist, A. Leder, and B. Norman 915
Organization of Immunoglobulin Genes S. Tonegawa, C. Brack, N. Hozumi, and V. Pirrotta 921
Studies on the Structure of Genes Expressed during Development G. K. Sim, A.. Efstratiadis, C. W. Jones, F. C. Kafatos, M. Koehler, H. M. Kronenberg, T. Maniatis, J. C. Regier, B. F. Roberts, and N. Rosenthal 933
Isolation and Characterization of the Silk Fibroin Gene with Its Flanking Se- quences Y. Suzuki and Y. Ohshima 947
Studies on the DNA Fragments of Mammals and Drosophila Containing Struc- tural Genes and Adjacent Sequences Y. V. Ilyin, N. A. Tchurikov, E. V. Ananiev, A. P. Ryskov, G. N. Yenikolopov, S. A. Limborska, N. E. Maleeva,, V. A. Gvozdev, and G. P. Georgiev 959
Characterization and Kinetics of Synthesis of 155 13-Globin RNA, a Putative Pre- cursor of b-Globin mENA P. J. Curtis, N. Mantei,, and C. Weissmann 971
Globin mRNA Sequences: Analysis of Base Pairing and Evolutionary Impli- cations W. Salser 985
Physical Mapping of Repetitive DNA Sequences Neighboring the Rabbit b-Globin Gene R. A. Flavell, A. J. Jeffreys, and G. C. Grosveld 1003
Cloned Eukaryotic Genes II
Gene Organization in Drosophila A. Chovnick, M. McCarron, A. Hilliker, J. O'Donnell, W. Gelbart, and S. Clark 1011
The Two-dimensional Fractionation of Drosophila DNA S. S. Potter and C. A. Thomas, Jr. 1023
Sequence Homology within Families of Drosophila melanogaster Middle Repeti- tive DNA P. C. Wensink 1033
Isolation of a Telomeric DNA Sequence from Drosophila melanogaster G. M. Rubin 1041
The Organization of the Histone Genes in Drosophila melanogaster: Functional and Evolutionary Implications R.. P. Lifton, M. L. Goldberg, R.. W. Karp, and D. S. Hogness 1047
Repeated Gene Families in Drosophila melanogaster D. J Finnegan, G. M Ru- bin,, M W Young, and D S. Hogness 1053
Short-period Repetitive-sequence Interspersion in Cloned Fragments of Sea Ur- chin DNA A. Shin Lee, R. J. Britten, and E. H. Davidson 1065
Histone Genes
Transcription of Xenopus tDNAMet and Sea Urchin Histone DNA Injected into the Xenopus Oocyte Nucleus A. Kressmann, S. G. Clarkson, J. L. Telford, and M. L. Birnstiel 1077
The Histone H4 Gene of Strongylocentrotus purpuratus: DNA and mRNA Se- quences at the 5' End M. Grunstein and J. B. Grunstein 1083
Histone Gene Heterogeneity in the Sea Urchin Strongylocentrotus purpuratus E. S. Weinberg, G. C. Overton, M. B. Hendricks, K. M. Newrock, and L. H. Cohen 1093
The Localization of the Genes Coding for Histone H4 in Human Chromosomes L. C. Yu, P. Szabo, T. W. Borun, and W. Prensky 1101
Regulation of Histone Gene Expression in HeLa S3 Cells G. S. Stein, J. L. Stein, W. D. Park, S. Detke, A. C. Lichtler, E. A. Shephard, R. L. Jansing and I. R.. Phillips 1107
Repeated DNA Sequences
Fine Structure and Evolution of DNA in Heterochromatin W. J. Peacock, A. R. Lohe, W. L. Gerlach, P. Dunsmuir, E. S. Dennis, and R. Appels 1121
DNA Sequence Organization of Drosophila Heterochromatin D. Brutlag, M. Carlson, K Fry, and T. S. Hsieh 1137
Mammalian Repetitive DNA and the Subunit Structure of Chromatin P. R.. Musich, F. L. Brown, and J. J. Maio 1147
Random Phasing of Polypyrimidine/Polypurine Segments and Nucleosome Mono- mers in Chromatin from Mouse L Cells H. C. Birnboim, R. M. Holford, and V. L. Seligy 1161
Ribosomal Genes and Their Proteins from Xenopus R.. H. Reeder, H. L. Wahn, P. Botchan, R. Hipskind, and B. Sollner-Webb 1167
A Study of Early Events in Ribosomal Gene Amplification A.. P. Bird 1179
Ribosomal DNA and Related Sequences in Drosophila melanogaster I. B. Dawid and P. K. Wellauer 1185
The Nucleotide Sequence of the Repeating Unit in the Qocyte 55 Ribosomal DNA of Xenopus laevis N. V. Fedoroff and D. D. Brown 1195
Simple Mendelian Inheritance of the Repeating Yeast Ribosomal DNA Genes T. D. Petes, L. M. Hereford, and D. Botstein 1201
Summary
The Molecular Biology of the Eukaryotic Genome Is Coming of Age P. Chambon 1209
Symposium Participants v
Foreword xv
Nucleosome Structure I: Crystallographic Analysis
X-ray and Electron Microscope Analyses of Crystals of Nucleosome Cores J. T. Finch and A. Klug 1
Scattering Studies of Chromatin Subunits J. F. Pardon, R. I. Cotter, D. M. J. Lilley, D. L. Worcester, A. M. Campbell, J. C. Wooley, and B. M. Richards 11
DNA Structure in Chromatin and in Solution Studied by Electron Microscopy and Neutron and X-ray Scattering S. Bram, S. Kouprach, and P. Baudy 23
Nucleosome Structure II: Conformational Studies
Conformational States of Chromatin M. Zama, P.N. Bryan, P. F. Harrington, A. L. Olins, and D. F. Olins 31
Ultrastructure of Chromatin Subunits during Unfolding, Histone Depletion, and Reconstruction C. L. F. Woodcock and L-L. Y. Frado 43
Nucleosome Structure, DNA Folding, and Gene Activity R. D. Camerini- Otero, B. Sollner- Webb, R.. H. Simon, P. Williamson, M. Zasloff and G. Felsenfeld 57
Internal Structure of the Nucleosome: DNA Folding in the Conserved 140-Base- pair Core Particle M. Noll 77
Conformational Flexibility in DNA Structure as Revealed by Structural Studies of Drug Intercalation and Its Broader Implications in Understanding the Organization of DNA in Chromatin H. M. Sobell, B. S. Reddy, K. K. Bhan- dary, S. C. Jain, T. D. Sakore, and T. P. Seshadri 87
Relation of Nucleosomes to DNA Sequences A. Prunell and R. D. Kornberg 103
Domains in Chromatin Structure T. 1gó-Kemenes and H G. Zachau 109
Nucleosome Structure III: The Core
The Nucleosome Core Protein J. O. Thomas and P. J. C. Butler 119
Histone-DNA Interactions in Chromatin Core Particles R. T. Simpson, J. P. Whitlock, Jr., M. Bina-Stein, and A. Stein 127
Characterization of DNase-I Cleavage Sites in the Nucleosome L. C. Lutter 137
The Structure of Nucleosomes: The Arrangement of Histones in the DNA Grooves and Along the DNA Chain A. D. Mirzabekov, V. V. Shick, A.. V. Belyavsky, V. L. Karpov, and S. G. Bavykin 149
Evolutionary Conservation of Histone-Histone Binding Sites: Evidence from In- terkingdom Complex Formation S. Spiker and I. Isenberg 157
Nucleohistone Assembly: Sequential Binding of Histone H3-H4 Tetramer and Histone H2A-H2B Dimer to DNA A. Ruiz-Carrillo and J. L. Jorcano 165
Chromatin Assembly and Transcription in Eggs and Qocytes of Xenopus lae- viz R.. A. Lasky, B. M. Honda, A.. D. Mills, N. R. Morris, A. H. Wyllie, J. E. Mertz, F. M. De Robertis, and J. B. Gurdon 171
Chromatin Structures of Main-band and Satellite DNAs in Drosophila melano- gaster Nuclei as Probed by Photochemical Cross-linking of DNA with Trioxsalen C.-K. J. Shen and J. F. Hearst 179
Chromatin Structure in Living Cells T. R. Cech, D. Potter, and M. L. Pardue Dynamics of Nucleosome Structure Studied by Fluorescence A. F. Dieterich, R.. Axel, and C. R.. Cantor 199
Electric Dichroism Studies of the Size and Shape of Nucleosomal Particles L. Klevan, M. Hogan, N. Dattagupta, and D. M. Crothers 207
Nucleosome Structure IV: H1 Interactions
The Multifunctional Role of Histone H1, Probed with the SV40 Mini- chromosome J. D. Griffith and G. Christiansen 215
Biochemical and Ultrastructural Analysis of SV40 Chromatin W. Keller, U. Müller, I. Eicken, I. Wendel, and H. Zentgraf 227
Histone Hi Involvement in the Structure of the Chromosome Fiber M. Renz, P. Nehls, and J. Hozie 245
H1 Histone and the Condensation of Chromatin and DNA R.. D. Cole, G. M. Lawson, and M. W. Hsiang 253
The Role of H1 in the Structure of Chromatin J. Gaubatz, R. Hardison, J. Murphy, M. E. Eichner, and R. Chalkley 265
Satellite DNA, Hi Histone, and Heterochromatin in Drosophila virus M. Blu- menfeld, J. W. Orf B. J. Sina, R. A. Kreber, M. A.. Callahan, and L. A. Snyder 273
Nucleosomes, Histone Interactions, and the Role of Histones H3 and H4 E. M. Bradbury, T. Moss, H. Hayashi, R. P. Hjelm, P. Suau, R. M. Stephens, J. P. Baldwin, and C. Crane-Robinson 277
Nucleosome Structure I: All Four Histones, H2A, H2B, H3, and H4, Are Required to Form a Nucleosome, but an H3-H4 Subnucleosomal Particle Is Formed with H3-H4 Alone P. Oudet, J. E. Germond, M. Sures, D. Gallwitz, M. Bel- lard, and P. Chambon 287
Nucleosome Structure II: Structure of the SV40 Minichromosome and Electron Microscopic Evidence for Reversible Transitions of the Nucleosome Struc- ture P. Oudet, C Spadafora, and P. Chambon 301
Chromosome Coiling and Assembly
Molecular Architecture of the Chromatin Fiber A. Worcel 313
v Bodies Are Close-packed in Chromatin Fibers A. L. Olins 325
A Direct Approach to the Structure of Eukaryotic Chromosomes J. Sedat and L. Manuelidis 331
Metaphase Chromosome Structure: The Role of Nonhistone Proteins U. K. Laemmli S. M. Cheng, K. W. Adolph, J. R.. Paulson, J. A. Brown, and W. R.. Baumbach 351
Metaphase Chromosome Architecture W. Wray, M. Mace, Jr., V. Daskal, and F. Stubblefield 361
Higher-order Structure of Mitotic Chromosomes A. Leth Bak and J. Zeuthen 367
Immunological Probes for Chromatin Structure M. Bustin, P. D. Kurth, E. N. Moudrianakis, D. Goldblatt, R. Sperling, and W. B. Rizzo 379
Functional Organization of Polytene Chromosomes M. Jamrich, A. L. Greenleaf F. A. Bautz and E. K. F. Bautz 389
Immunochemical Studies on the 5-Methylcytosine Content of African Green Mon- key Satellite DNA Z. F. Kahana, O. J. Miller, and B. F. Erlanger 397
The Generation and Propagation of Variegated Chromosome Structures H. Weintraub, S. J. Flint, I. M. Leffak, M. Groudine, and R.. M. Grainger 401
Assembly of SV40 and Polyoma Minichromosomes during Replication C. Cré- misi, A. Chestier, and M. Yani 409
Segregation of Some Chromosomal Proteins during Cell Replication E. F. Freed- lender, L. Taichman, and O. Smithies 417
Histone Changes during Chromatin Remodeling in Embryogenesis K. M. New- rock, C. R. Alfageme, R.. V. Nardi, and L. H. Cohen 421
Persistence of Nucleosomes on DNA during Chromatin Replication R. L. Seale 433
Simple Systems
Localization of the HU Protein on the Escherichia coli Nucleoid J. Rouvière- Yani 439
The Structure of Genes, Intergenic Sequences, and mRNA from SV40 Virus B. Thimmappaya, V. B. Reddy, R. Dhar, M. Celma, K. N. Subramanian, B. S. Zain, J. Pan, and S. M. Weissman 449
On the Structure of Eukaryotic, Prokaryotic, and Viral Chromatin A. J. Var- shavsky, V. V. Bakayev, S. A. Nedospasov, and G. P. Georgiev 457
Histone Organization in Chromatin: Comparisons of Nucleosomes and Subnucleo- somal Particles from Erythrocyte, Myeloma, and Yeast Chromatin R. L. Rill and D. A. Nelson 475
The Gene-size DNA Molecules in Oxytricha R. M. Lawn, J. M. Heumann, G. Herrick, and D. M. Prescott 483
Histones and Chromatin Structure in Tetrahymena Macro- and Micronuclei M. A. Gorovsky, C. Glover, C. A. Johmann, J. B. Keevert, D. J. Mathis, and M. Samuelson 493
Structural Organization and Control of the Ribosomal Genes in Physarum V. G. Allfrey, E. M. Johnson, I. Y.-C. Sun, V. C. Littau, H. R.. Matthews, and E. M. Bradbury 505
Transcription I: Primary Products
The Definition of Transcription Units for mRNA eli B. Darnell, R. Evans, N. Fraser, S. Goldberg J. Nevins, M. Salditt-Georgieff H. Schwartz, J. Weber, and B. Ziff 515
Spliced Segments at the 5' Termini of Adenovirus-2 Late mRNA: A Role for Heterogeneous Nuclear RNA in Mammalian Cells S. M. Berget, A. J. Berk, T. Harrison, and P. A. Sharp 523
Adenovirus-2 Messengers - An Example of Baroque Molecular Architecture T. R.. Broker, L. T. Chow, A.. R. Dunn, R. E. Gelinas, J. A. Hassell, D. F. Klessig, J. B. Lewis, R. J. Roberts, and B. S. Zain 531
Displacement Loops in Adenovirus DNA-RNA Hybrids H. Westphal and S.-P. Lai 555
Splicing of SV40 mRNAs: A Novel Mechanism for the Regulation of Gene Expres- sion in Animal Cells Y. Aloni, S. Bratosin, R. Dhar, O. Laub, M. Horowitz, and G. Khoury 559
A Novel Sequence Arrangement of SV40 Late RNA M.-T. Hsu and J. Ford 571
Faithful Gene Transcription by Eukaryotic RNA Polymerases in Reconstructed Systems C. S. Parker, J. A. Jaehning and R. G. Roeder 577
Studies on Gene Transcription In Vitro by Analysis of the Primary Tran- scripts R. C. C. Huang, M. M. Smith, and A.. E. Reeve 589
Properties of Transcriptionally Active Chromatin J. Paul, E. J. Zollner, R. S. Gilmour, and G. D. Birnie 597
PART 2
Transcription II: Regulated Systems
Regulation of Gene Expression in Chick Oviduct B. W. O'Malley, M.-J. Tsau, S. Y. Tsai and H. C. Towle 605
Hormonal Control of Egg White Protein Messenger RNA Synthesis in the Chicken Oviduct G. Shütz, M. C. Nguyen-Huu, K Giesecke, N. B. Hynes, B. Groner, T. Wurtz, and A. E. Sippel 617
Mammary Tumor Virus DNA: A Glucocorticoid-responsive Transposable Ele- ment K. R.. Yamamoto, M. R. Stallcup, J. Ring, and G. M. Ringold 625
Regulation of Gene Expression in the Chick Oviduct by Steroid Hormones R. D. Palmiter, B. R. Mulvihill, G. S. McKnight, and A. W. Senear 639
Amplification of Folate Reductase Genes in Methotrexate-resistant Cultured Mouse Cells R. T. Schimke, F. W. Alt, R. E. Kellems, R. Kaufman, and J. B. Bertino 649
Hormonal Modulation of a 2u Globulin mRNA: Sequence Measurements Using a Specific cDNA Probe P. Feigelson and D. T. Kurtz 659
The Nucleolus, a Model for Analysis of Chromatin Controls H. Busch, N. R. Ballal, R. K. Busch, Y. C. Choi, F. Davis, I. L. Goldknopf, S.-I. Matsui, M. S. Rao, and L. I. Rothblum 665
Stimulation of Ribosomal RNA Synthesis in Isolated Nuclei and Nucleoli by Partially Purified Preparations of SV40 T Antigen R. Baserga,, T. Ide, and S. Whelly 685
Purification of a Protein from Unfertilized Eggs of Drosophila with Specific Affin- ity for a Defined DNA Sequence and the Cloning of This DNA Sequence in Bacterial Plasmids H. Weideli, P. Schedl, S. Artivanis-Tsakonas, R.. Steward, R. Yuan, and W. J. Gehring 693
Transcriptionaily Active Chromatin
The Structure of the Transcriptionally Active Ovalbumin Genes in Chroma tin A.. Garel and R.. Axel 701
Structure of Xenopus Ribosomal Gene Chromatin during Changes in Genomic Transcription Rates R. Reeves 709
Modulation of Ribosomal RNA Synthesis in Oncopeltus fasciatus: An electron Microscopic Study of the Relationship between Changes in Chromatin Struc- ture and Transcriptional Activity V. E. Foe 723
Electron Microscopic Analysis of Chromosome Metabolism in the Drosophila me- lanogaster Embryo S. L. McKnight, M. Bustin, and O. L. Miller, Jr 741
Morphology of Transcriptionally Active Chromatin W. W. Franke U Scheer, M. Trendelenburg, H. Zentgraf and H. Spring 755
Structure of rDNA-containing Chromatin of Tetrahymena pyriformis Analyzed by Nuclease Digestion D. J. Mathis and M. A. Gorovsky 773
Nucleosome Structure III: The Structure and Transcriptional Activity of the Chromatin Containing the Ovalbumin and Globin Genes in Chick Oviduct Nuclei M. Bellard, F. Gannon, and P. Chambon 779
Structure and Function of the Low-salt Extractable Chromosomal Proteins. Pref- erential Association of Trout Testis Proteins H6 and HMG-T with Chromatin Regions Selectively Sensitive to Nucleases B. Levy W., N. C. W. Wong D.C. Watson, E.H. Peters, and G.H. Dixon 793
Transcription of Polytene Chromosomes and of the Mitochondrial Genome in Drosophila melanogaster J. J. Bonner, M. Berninger, and M. L. Pardue 803
Modified Histones in HeLa and Friend Erythroleukemia Cells Treated with n- Butyrate M. G. Riggs, R.. G. Whittaker, J. R. Neumann, and V. M. Ingram 815
The Effect of Heat Shock on Gene Expression in Drosophila melanogaster M.-E.. Mirault, M. Goldschmidt-Clermont, L. Moran, A. P. Arrigo, and A. Tissières 819
Correlation of Structural Changes in Chromatin with Transcription in the Drosophila Heat-shock Response H. Biessmann, S. Wadsworth, B. Levy W., and B. J. McCarthy 829
An In Vitro Assay for the Specific Induction and Regression of Puffs in Iso- lated Polytene Nuclei of Drosophila melanogaster J. L. Compton and J. J. Bonner 835
Distribution Patterns of Drosophila Nonhistone Chromosomal Proteins S. C. R.. Elgin, L. A. Serunian, and L. M. Silver 839
The Expressed Portion of Eukaryotic Chromatin J. Bonner, R. B. Wallace, T. D. Sargent, R. F. Murphy, and S. K. Dube 851
"Native" Salivary Chromosomes of Drosophila melanogaster R. J. Hill and F. Watt 859
The Size and Chromosomal Location of the 755
RNA Transcription Unit in Balbiani Ring 2 B. Daneholt, S. T. Case, J. Derksen, M. M. Lamb, L. Nelson, and L. Wieslander 867
Fate of Balbiani-ring RNA In Vivo J.-E. Edström,, E. Ericson, S. Lindgren, U. Lönn, and L. Rydlande 877
The Packaging Proteins of Core hnRNP Particles and the Maintenance of Prolif- erative Cell States W. M. LeStourgeon, A. L. Beyer, M. E. Christensen, B. W. Walker, S. M. Poupore, and L. P. Daniels 885
Substructure of Nuclear Ribonucleoprotein Complexes T. Martin, P. Billings, J. Pullman, B. Stevens, and A. Kinniburgh 899
Free Informofers and Reconstitution of 30S RNP V. V. Kulguskin, E. M. Lukani- din, and G. P. Georgiev 911
Cloned Eukaryotic Genes I
The Cloning of Mouse Globin and Surrounding Gene Sequences in Bacterio- phage l P. Leder, S. M. Tilghman, D.C. Tiemeier, F. I. Polsky, J. G. Seidman, M. H. Edgell, L. W. Enquist, A. Leder, and B. Norman 915
Organization of Immunoglobulin Genes S. Tonegawa, C. Brack, N. Hozumi, and V. Pirrotta 921
Studies on the Structure of Genes Expressed during Development G. K. Sim, A.. Efstratiadis, C. W. Jones, F. C. Kafatos, M. Koehler, H. M. Kronenberg, T. Maniatis, J. C. Regier, B. F. Roberts, and N. Rosenthal 933
Isolation and Characterization of the Silk Fibroin Gene with Its Flanking Se- quences Y. Suzuki and Y. Ohshima 947
Studies on the DNA Fragments of Mammals and Drosophila Containing Struc- tural Genes and Adjacent Sequences Y. V. Ilyin, N. A. Tchurikov, E. V. Ananiev, A. P. Ryskov, G. N. Yenikolopov, S. A. Limborska, N. E. Maleeva,, V. A. Gvozdev, and G. P. Georgiev 959
Characterization and Kinetics of Synthesis of 155 13-Globin RNA, a Putative Pre- cursor of b-Globin mENA P. J. Curtis, N. Mantei,, and C. Weissmann 971
Globin mRNA Sequences: Analysis of Base Pairing and Evolutionary Impli- cations W. Salser 985
Physical Mapping of Repetitive DNA Sequences Neighboring the Rabbit b-Globin Gene R. A. Flavell, A. J. Jeffreys, and G. C. Grosveld 1003
Cloned Eukaryotic Genes II
Gene Organization in Drosophila A. Chovnick, M. McCarron, A. Hilliker, J. O'Donnell, W. Gelbart, and S. Clark 1011
The Two-dimensional Fractionation of Drosophila DNA S. S. Potter and C. A. Thomas, Jr. 1023
Sequence Homology within Families of Drosophila melanogaster Middle Repeti- tive DNA P. C. Wensink 1033
Isolation of a Telomeric DNA Sequence from Drosophila melanogaster G. M. Rubin 1041
The Organization of the Histone Genes in Drosophila melanogaster: Functional and Evolutionary Implications R.. P. Lifton, M. L. Goldberg, R.. W. Karp, and D. S. Hogness 1047
Repeated Gene Families in Drosophila melanogaster D. J Finnegan, G. M Ru- bin,, M W Young, and D S. Hogness 1053
Short-period Repetitive-sequence Interspersion in Cloned Fragments of Sea Ur- chin DNA A. Shin Lee, R. J. Britten, and E. H. Davidson 1065
Histone Genes
Transcription of Xenopus tDNAMet and Sea Urchin Histone DNA Injected into the Xenopus Oocyte Nucleus A. Kressmann, S. G. Clarkson, J. L. Telford, and M. L. Birnstiel 1077
The Histone H4 Gene of Strongylocentrotus purpuratus: DNA and mRNA Se- quences at the 5' End M. Grunstein and J. B. Grunstein 1083
Histone Gene Heterogeneity in the Sea Urchin Strongylocentrotus purpuratus E. S. Weinberg, G. C. Overton, M. B. Hendricks, K. M. Newrock, and L. H. Cohen 1093
The Localization of the Genes Coding for Histone H4 in Human Chromosomes L. C. Yu, P. Szabo, T. W. Borun, and W. Prensky 1101
Regulation of Histone Gene Expression in HeLa S3 Cells G. S. Stein, J. L. Stein, W. D. Park, S. Detke, A. C. Lichtler, E. A. Shephard, R. L. Jansing and I. R.. Phillips 1107
Repeated DNA Sequences
Fine Structure and Evolution of DNA in Heterochromatin W. J. Peacock, A. R. Lohe, W. L. Gerlach, P. Dunsmuir, E. S. Dennis, and R. Appels 1121
DNA Sequence Organization of Drosophila Heterochromatin D. Brutlag, M. Carlson, K Fry, and T. S. Hsieh 1137
Mammalian Repetitive DNA and the Subunit Structure of Chromatin P. R.. Musich, F. L. Brown, and J. J. Maio 1147
Random Phasing of Polypyrimidine/Polypurine Segments and Nucleosome Mono- mers in Chromatin from Mouse L Cells H. C. Birnboim, R. M. Holford, and V. L. Seligy 1161
Ribosomal Genes and Their Proteins from Xenopus R.. H. Reeder, H. L. Wahn, P. Botchan, R. Hipskind, and B. Sollner-Webb 1167
A Study of Early Events in Ribosomal Gene Amplification A.. P. Bird 1179
Ribosomal DNA and Related Sequences in Drosophila melanogaster I. B. Dawid and P. K. Wellauer 1185
The Nucleotide Sequence of the Repeating Unit in the Qocyte 55 Ribosomal DNA of Xenopus laevis N. V. Fedoroff and D. D. Brown 1195
Simple Mendelian Inheritance of the Repeating Yeast Ribosomal DNA Genes T. D. Petes, L. M. Hereford, and D. Botstein 1201
Summary
The Molecular Biology of the Eukaryotic Genome Is Coming of Age P. Chambon 1209
Search images:
