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 XXXIX: Tumor Viruses 1974 |
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From left: Dr. Sol Spiegelman, Dr. Stuart Aaronson  SYMPOSIUM SYNOPSIS LIST OF PARTICIPANTS CONTENTS OF SYMPOSIUM VOLUME PHOTOGRAPHS PUBLISHED VOLUME  |
Tumor Viruses
Symposium Volume XXXIX Contents
PART 1
Symposium Participants v
Foreword xvii
Opening Remarks
Oncogenic Viruses: The Last Twelve Years R. Dulbecco 1
SV40 AND POLYOMA
Genome Structure and Genetics
Altered Patterns of Protein Synthesis in Infection by SV40 Mutants P. Tegt meyer 9
The Semiautonomous Replicon: A Molecular Model for the Oncogenicity of SV40 R. G. Martin, J. Y. Chou, J. Avila and R. Saral 17
Transformation of Primate and Rodent Cells by Temperature-sensitive Mutants of SV40 J. S. Butel, J. S. Brugge and C. A. Noonan 25
Properties of Temperature-sensitive Mutants of Polyoma Virus W. Eckhart 37
Indirect Complementation of a Nontransforming Mutant of Polyoma Virus T. L. Benjamin and E. Goldman 41
Polyoma Virus - A Study of Wild-type, Mutant and Defective DNAs M. Fried, B. E. Griffin, E. Lund and D. L. Robberson 45
Mapping the Genes of Simian Virus 40 C..J. Lai and D. Nathans 53
Mapping of Mutational Alterations in DNA with Si Nuclease: The Location of Deletions, Insertions and Temperature-sensitive Mutations in SV40 T. E. Shenk, C. Rhodes, P. W. J. Rigby and P. Berg 61
Isolation and Characterization of Individual Clones of Simian Virus 40 Mutants Containing Deletions, Duplications and Insertions in Their DNA J. E. Mertz, J. Carbon, M. Herzberg, R. W. Davis and P. Berg 69
Characterization of Defective Simian Virus 40 DNA: Comparison between Large- plaque and Small-plaque Types K. Yoshiike, A. Furuno, S. Watanabe, S. Uchida, K. Matsubara and Y. Takagi 85
Characterization of SV40 DNA Rescued from Transformed Mouse Cells M. Botchan, B. Ozanne and J. Sambrook 95
Host Substitution in SV40 and Polyoma DNA E. Winocour, N. Frenkel, S. Lavi, M. Osenholts and S. Rozenblatt 101
Electron Microscopic Mapping of the Attachment Sites on SV40 DNA during Lytic Infection L. T. Chow, H. W. Boyer, E. G. Tischer and H. M. Goodman 109
Characterization of Cloned Evolutionary Variants of Simian Virus 40 W. W. Brockman, T. N. H. Lee and D. Nathans 119
Specific Reiteration of Viral DNA Sequences in Mammalian Cells M. A. Martin, G. Khoury and G. C. Fareed 129
Formation of Reiterated Simian Virus 40 DNA D. Davoli and G. C. Fareed 137
The Detection and Quantitation of SV40 Nucleic Acid Sequences Using Single- stranded SV40 DNA Probes G. Khoury, P. Howley, M. Brown and M. Martin 147
RNA Transcription
Nucleotide Sequence about the 3¢ Terminus of SV40 DNA Transcripts and the Region Where DNA Synthesis Is Initiated R. Dhar, K. Subramanian, B. S. Zain, J. Pan and S. M. Weissman 153
Mapping of SV40 mRNA Species on the Viral Genome R. A. Weinberg and J. E. Newbold 161
Biogenesis and Characterization of SV40 and Polyoma RNAs in Productively Infected Cells Y. Aloni 165
Approaches to the Sequence Determination of SV40 DNA W. Fiers, K. Danna, R. Rogiers, A. Vandevoorde, J. Van Herreweghe, H. Van Heuverswyn, G. Volckaert and R. Yang 179
Virus-specific RNA in Cells Productively Infected or Transformed by Polyoma Virus R. Kamen, D. M. Lindstrom, H. Shure and R. W. Old 187
Replication
Stepwise Relaxation of Supercoiled SV40 DNA W. Keller and I. Wendel 199
Properties of Replicating SV40 DNA Molecules and Mapping Unpaired Regions in SV40 DNA I N. P. Salzman, J. Lebowitz, M. Chen, E. Sebring and C. F. Garon 209
Polyoma DNA Replication: Location of the Origin in Different Virus Strains L. V. Crawford, A. K. Robbins, P. M. Nicklin and K. Osborn 219
Replication of Polyoma DNA: Effects of Hydroxyurea and Arabinosyl Cytosine G. Magnusson, R. Craig, M. Närkhammar, P. Reichard, M. Staub and H. War- ner 227
Proteins and Their Synthesis
Fingerprints of Polyoma Virus Proteins and Mouse Histones G. Fey and B. Hirt 235
Virion Proteins of Polyoma Temperature-sensitive Mutants: Late Mutants T. Friedmann and W. Eckhart 243
Endonucleolytic Cleavage of Polyoma Virus DNA: General Properties and Site Specificity of the Virion-associated Endonuclease A. Parodi, P. Rouget, O. Croissant, D. Blangy and F. Cuzin 247
Intermolecular Disulfide Bonds: An Important Structural Feature of the Polyoma Virus Capsid G. Walter and W. Deppert 255
The Organization of Proteins in Polyoma and Cellular Chromatin A. J. Louie 259
SV40: T Antigen, the A Function and Transformation M. Osborn and K. Weber 267
Regulation of 5V40 Tumor Antigen in Transformed Mouse 3T3 Cells J. A. Robb 277
SV40 T Antigen: Partial Purification and Properties D. M. Livingston, I. C. Henderson and J. Hudson 283
Dissociation and DNA Binding of SV40 T Antigen R. B. Carroll, L. Hager and R. Dulbecco 291
An Antigen Associated with Messenger RNA in a Transformed Hamster Cell Line M.-E. Mirault, S. I. Reed and G. R.Stark 295
Cytosine Arabinoside- and Interferon-mediated Control of Polyoma and SV40 Genome Expression G. Brandner and N. Mueller 305
The Cell-free Translation of SV40 Messenger RNA C. L. Prives, H. Aviv, E. Gil- boa, M. Revel and E. Winocour 309
Transformed Cells
The Stable Classes of Transformed Cells Induced by SV40 Infection of Esta- blished 3T3 Cells and Primary Rat Embryonic Cells R. Risser, D. Ri/kin and R. Pollack 317
Factors Affecting the Process and Extent of Integration of the Viral Genome K. Hirai and V. Defendi 325
Genetics of Cell Transformation by Simian Virus 40 C. M. Croce, K. Huebner, A. J. Girardi and H. Koprowski 335
Variants of Simian Virus 40-transformed Mouse Cells Resistant to Cytochalasin B F. Kelly and J. Sambrook 345
The SV40 Rescue Problem J. F. Watkins 355
Localization and Distribution of Actin Fibers in Normal, Transformed and Re- vertant Cells K. Weber, E. Lazarides, R. D. Goldman, A. Vogel and R. Pol- lack 363
Differential Susceptibility to Amphotericin B of 3T3 Cells Transformed by SV40 or Polyoma P. Amati and C. Lago 371
Infection of Mouse Blastocysts with SV40 DNA: Normal Development of the In- fected Embryos and Persistence of SV40-specific DNA Sequences in the Adult Animals R. Jacnisch 375
A Simplifying Concept in Tumor Virology: Virus-specific "Pleiotropic Effectors" R. Weil, C. Salomon, E. May and P. May 381
ADENO VIRUSES
Genome Structure and Genetics
Cleavage Maps of DNA from Adenovirus Types 2 and 5 by Restriction Endo- nucleases EcoRI and HpaI C. Mulder, J. R. Arrand, H. Delius, W. Keller, U. Pettersson, R. J. Roberts and P. A. Sharp 397
Extent of Terminal Repetition in Adenovirus 2 DNA J. R. Arrand, W. Keller and R. J. Roberts 401
Structural Studies on Two Adenovirus 2-SV40 Hybrids Which Contain the En- tire 5V40 Genome T. J. Kelly, Jr., A. M. Lewis, Jr., A. S. Levine and S. Siegel 409
Cell Transformation: A Study of Regulation with Types S and 12 Adenovirus Temperature-sensitive Mutants H. S. Ginsberg, M. J. Ensinger, R. S. Kauff- man, A. J. Mayer and U. Lundholm 419
Genetic Analysis of Human Adenovirus Type 5 in Permissive and Nonpermissive Cells J. F. Williams, C. S. H. Young and P. E. Austin 427
Physical Mapping of Temperature-sensitive Mutations of Adenoviruses T. Grodzicker, J. Williams, P. Sharp and J. Sambrook 439
Transcription
RNA Synthesis and Processing in Adenovirus-infected Cells L. Philipson, U. Pettersson, U. Lind berg, C. Tibbetts, B. Vennstrom and T. Persson 447
Mapping of Adenovirus 2 RNA Sequences in Lytically Infected Cells and Trans- formed Cell Lines P. A. Sharp. P. H. Gallimore and S. J. Flint 457
An Examination of the Nuclear RNA of Adenovirus-transformed Cells M. Gear- gieff S. Bachenheimer and J. E. Darnell 475
RNA Transcription in Cultures Productively Infected with Adenovirus 2 E. A. Craig, J. Tal, T. Nishimoto, S. Zimmer, M. MeGrogan and H. J. Raskas 483
The Transcriptional Role of Host DNA-dependent RNA Polymerases in Adeno- virus-infected KB Cells R. Weinmann, H. J. Raskas and R. G. Roeder 495
In Vitro Transcription of Adenovirus 2 DNA byE. coli RNA Polymerase S. Sur- zycki, J. A. Surzycki, W. DeLorbe and G. N. Gussin 501
Replication
Integration of Adenovirus DNA into the Cellular Genome W. Doer/ler, H. Bur- ger, J. Ortin, E. Fanning, D. T. Brown, M. Westphal, U. Winterhoff, B. Weiser and J. Schick 505
A Circular DNA-Protein Complex from Adenoviruses and Its Possible Role in DNA Replication A. J. Robinson and A. J. D. Bellett 523
The Viral DNA Replication Machinery of Adenovirus 12 H. Shimojo, K. Shiroki and K. Yamaguchi 533
The Mechanism of Replication of Adenovirus Type 5 DNA J. S. Sussenbach, D. J. Ellens, P. Ch. van der Vliet, M. G. Kuijk, P. H. Steenbergh, J. M. VIak, Th. H. RozUn and H. S. Jansz 539
Origins and Termini of Adenovirus Type 2 DNA Replication E. L. Winnacker 547
Proteins and Their Synthesis
Early Events in Adenovirus-infected Cells W. C. Russell 551
Adenovirus-infected, Cell-specific, DNA-binding Proteins A. J. Levine, P. C. van der Vlict, B. Rosenwirth, J. Rabek, G. Frenkel and M. Ensinger 559
Human Adenovirus Infection in Monkey Cells: An Example of Host Restriction at a Step Late in Replication S. G. Baum and R. I. Fox 567
Analysis of Adenovirus Type 2 Gene Functions by Cell-free Translation of Viral Messenger RNA H. Westphal, L. Eron, F.-J. Ferdinand, R. Callahan and S-P. Lai 575
The Origin and Destiny of Adenovirus Proteins J. B. Lewis, C. W. Anderson, J. F. Atkins and R. F. Gesteland 581
Transformed Cells
Recent Studies on the Characteristics of Adenovirus-infected and -transformed Cells J. K. McDougall, A. R. Dunn and P. H. Gallimore 591
Properties and Behavior of Hamster Embryo Cells Transformed by Human Ad- enovirus Type 5 R. D. Goldman, C. Chang and J. F. Williams 601
Viral DNA Sequences in Cells Transformed by Simian Virus 40, Adenovirus Type 2 and Adenovirus Type 5 J. Sambrook, M. Botchan, P. Gallimore, B. Ozanne, U. Pettersson, J. Williams and P. A. Sharp 615
Analysis of Multiple Viral Genome Fragments in Adenovirus 7-transformed Hamster Cells K. Fujinaga, K. Sekikawa, H. Yamazaki and M. Green 633
Studies on In Vitro Transformation by DNA and DNA Fragments of Human Adenoviruses and Simian Virus 40 F. L. Graham, P. J. Abrahams, C. Mul- der, H. L. He~neker, 5. 0. Warnaar, F. A. J. de Vries, W. Fiers and A. J. van der Eb 637
Studies of Hamster Cells Transformed by Adenovirus 2 and the Nondefective Ad2-SV40 Hybrids A. M. Lewis, Jr., J. H. Breeden, Y. L. Wewerka, L. E. Schnipper and A. S. Levine 651
PART 2
HERPES VIRUSES
Herpes Simplex
The Structure and Biological Properties of Herpes Simplex Virus DNA N. M. Wilkie, J. B. Clements, J. C. M. Macnab and J. H. Subak-Sharpe 657
Inverted Repetitions in the Chromosome of Herpes Simplex Virus P. Sheldrick and N. Berthelot- 667
Terminal Repetitions in Herpes Simplex Virus Type 1 DNA R. H. Grafstrom, J. C. Alwine, W. L. Steinhart and C. W. Hill 679
Analysis of DNA of Defective Herpes Simplex Virus Type 1 by Restriction Endo- nuclease Cleavage and Nucleic Acid Hybridization M. Wagner, J. Share and W. C. Summers 683
Regulation of Herpesvirus Macromolecular Synthesis: Evidence for Multilevel Regulation of Herpes Simplex 1 RNA and Protein Synthesis B. Roizman, M. Kozak, R. W. lioness and G. Hayward 687
Thymidine Kinase Isozymes of Normal and Virus-infected Cells S. Kit, W.-C. Leung, G. N. Jorgensen, D. Trkula and D. R. Dubbs 703
Genetic and Biochemical Studies with Herpesvirus J. H. Subak-Sharpe, S. M. Brown, D. A. Ritchie, M. C. Timbu,y, J. C. M. Macnab, H. S. Marsden and J. Hay 717
Viral Gene Functions Expressed and Detected by Temperature-sensitive Mutants of Herpes Simplex Virus M. Benyesh-Melnick, P. A. Schaffer, R. J. Courtney, J. Esparza and S. Kimura 731
Demonstration of the Oncogenic Potential of Herpes Simplex Viruses and Human Cytomegalovirus F. Rapp and J.-L. H. Li 747
Transformation of Cells by Herpes Simplex Virus - Fact or Fantasy? B. R. Mc- Auslan, B. Gar/inkle, R. Adler, D. Devinney, R. Florkiewicz and J. E. Shaw 765
Epstein-Barr Virus
Immortalizing and Nonimmortalizing Laboratory Strains of Epstein-Barr Virus G. Miller, J. Robinson and L. Heston 773
Studies on the Epstein-Barr Virus Genome and the EBV-determined Nuclear Antigen in Human Malignant Disease G. Klein 783
EBV DNA in Nonlymphoid Cells of Nasopharyngeal Carcinomas and in a Malig- nant Lymphoma Obtained after Inoculation of EBV into Cottontop Marmo- sets H. Wolf J. Werner and H. zur Hausen 791
The Epstein-Barr Virus and Malignancy: Molecular Evidence J. S. Pagano 797
Plasmid DNA as a Possible State of Epstein-Barr Virus Genomes in Nonproduc- tive Cells M. Nonoyama and A. Tanaka 807
Sequence of Epstein-Barr Virus Productive Cycle in Human Lymphoblastoid Cells B. Hampar, J. G. Derge, A. Tanaka and M. Nonoyama 811
Response of B and T Lymphocytes and Other Blood Leukocytes in Chickens with Marek's Disease L. N. Payne, P. C. Powell and M. Rennie 817
Organization of Virion RNA Genome and Genetics
Electron Microscope Studies of Tumor Virus RNA H. J. Kung, J. M. Bailey, N. Davidson, P. K. Vogt, M.O. Nicolson and R. M. McAllister 827
Electron Microscope Measurements of Rous Sarcoma Virus RNA H. Delius, P. H. Duesberg and W. F. Mangel 835
Determination of the Molecular Weight of the RNA Subunits of Rous Sarcoma Virus by Electron Microscopy A. B. Jacobson and P. A. Bromley 845
Avian RNA Tumor Viruses: Mechanism of Recombination and Complexity of the Genome P. Duesberg, P. K. Vogt, K. Beemon and M. Lai 847
35S a and b RNA Subunits of Avian RNA Tumor Virus Strains Cloned and Pas- saged in Chick and Duck Cells M. P. Stone, R. E. Smith and W. K. ,Joklik 859
Base Sequence Complexity of 35S Avian Myeloblastosis Virus RNA Determined by Molecular Hybridization Kinetics M. A. Baluda, M. Shoyab, P. D. Mark- ham, R. M. Evans and W. N. Drohan 869
Studies of the Interrelationship of Chicken Leukosis Virus and Host Cell Genomes by RNA-DNA Hybridization P. E. Neiman, S. E. Wright and H. G. Purchase 875
A Biochemical and Genetic Analysis of Mammalian RNA-containing Sarcoma Viruses E. M. Scolnick, R. J. Goldberg and W. P. Parks 885
Genetic Recombination among Temperature-sensitive Mutants of Rous Sarcoma Virus J. A. Wyke, J. G. Bell and J. A. Beamand 897
Phenotypically Mixed Vesicular Stomatitis Virus Particles Produced in Human Tumor Cell Lines J. Zavada, J. Bubenik, R. Widmaicr and Z. Zavadova 907
Phenotypic Mixing between Vesicular Stomatitis Virus and Avian RNA Tumor Viruses R. A. Weiss, D. Boettiger and D. N. Love 913
Reverse Transcriptase
Role of the Subunits of the Avian RNA Tumor Virus Reverse Transcriptase A. Panet, I. M. Verma and D. Baltimore 919
Structure and Properties of an RNA Primer for Initiation of Rous Sarcoma Virus DNA Synthesis In Vitro J. E. Dahlberg, F. Harada and R. C. Sawyer 925
Relationships between Components in Primate RNA Tumor Viruses and in the Cytoplasm of Human Leukemic Cells: Implications to Leukemogenesis R. C. Gallo, R. E. Gallagher, N. R. Miller, H. Mondal, W. C. Saxinger, R. J. Mayer, R. G. Smith and D. H. Gillespie 933
Reverse Transcriptase Associated with A-type Particles from Murine Myeloma Cells R. E. Thach, D. L. Robertson, N. L. Baenziger and D. C. Dobbertin 963
Reverse Transcriptase and RNase H: Present in a Murine Virus and in Both Sub- units of an Avian Virus K. Moelling 969
Proviral DNA and Its Transcription
Properties of Oncornavirus RNA-directed DNA Polymerase, the RNA Template, and the Intracellular Products Formed Early during Infection and Cell Transformation M. Green, D. Grandgenett, G. Gerard, H. M. Rho, M. C. Loni, M. Robins, S. Salzberg, G. Shanmugam, S. Bhaduri and G. Vecchio 975
Synthesis, Structure and Function of Avian Sarcoma Virus-specific DNA in Permissive and Nonpermissive Cells H. E. Varmus, R. V. Guntaka, C. T. Deng and J. M. Bishop 987
The Genome of RNA Tumor Viruses: A Functional Requirement for a Polyploid Structure? P. D. Cooper and J. A. Wyke 997
Studies on Characterization of the Integration Sites of Avian RNA Tumor Virus- specific DNA R. M. Evans, M. Shoyab and M. A. Baluda 1005
DNA-RNA Hybrid in Cells Infected by Murme Leukemia Virus T. Takano and M. Hatanaka 1009
Infectious Viral DNA in Rous Sarcoma Virus-transformed Nonproducer and Producer Animal Cells M. Hill, J. Hillova, D. Dantchev, R. Manage and G. Goubin 1015
Infectious DNA from Cells Infected with Rous Sarcoma Virus, Reticulcendothe- liosis Virus or Rous-associated Virus-0 G. M. Cooper and H. M. Temin 1027
AMV RNA Transcription in Cell-free Systems and Properties of In Vitro Chro- matin-directed RNA Synthesis G. Monroy, M. Jacquet, Y. Groner and J. Hurwitz 1033
Studies on the Structure and Synthesis of Rous Sarcoma Virus RNA C. Weiss- mann, J. T. Parsons, J. W. Coffin, L. Rymo, M. A. Billeter and H. Hofstetter 1043
Proteins
Characterization of Murine Leukemia Virus-specific Proteins E. Fleissner, H. Ikeda, J-S. Tung, E. S. Vitetta, E. Tress, W. Hardy, Jr., E. Stockert, E. A. Boyse, T. Pincus and P. O'Donnell 1057
Synthesis of Avian RNA Tumor Virus Structural Proteins R. Eisenman, V. M. Vogt and H. Diggelmann 1067
Expression of Endogeneous Viruses
Problems of RSV Rescue from Virogenic Mammalian Cells J. Svododa, I. Hlo žánek, O. Mach and S. Zadražil 1077
Qualitative and Quantitative Studies of AKR-type Murine Leukemia Virus Sequences in Mouse DNA S. K. Chattopadhyay, D. R. Lowy, N. M. Teich, A. S. Levine and W. P. Rowe 1085
Mechanism of Induction of RNA Tumor Viruses by Halogenated Pyrimidines P. Besmer, D. Smotkin, W. Haseltine, H. Fan, A. T. Wilson, M. Paskind, R. Weinberg and D. Baltimore 1103
Structural Proteins of RNA Tumor Viruses as Probes for Viral Gene Expression M. Strand and J. T. August 1109
Gen etic Control of the Expression of Murine Leukemia Virus Proteins in Tissues of Normal, Young Adult Mice M. Strand, F. Lilly and J. T. August 1117
Immune Response in the Mouse to Endogenous Leukemia Viruses R. C. Nowin- ski, S. L. Kaehler and R. R. Burgess 1123
Differential Cellular Regulation of Three Distinct Classes of Type C RNA Viruses Endogenous to Mouse Cells S. A. Aaronson and J. R. Stephenson 1129
Control of Expression of Tumor Virus Genes in Uninfected Chicken Cells H. Hanaf~tsa, W. S. Hayward, J. H. Chen and T. Hanafusa 1139
Endogenous Mammary Tumor Viruses in Mice P. Bentvelzen 1145
Glucocorticoid Induction of Murine Mammary Tumor Virus In Vitro W. P. Parks, E. M. Scolnick and J. C. Ransom 1151
Endogenous Primate and Feline Type C Viruses G. J. Todaro, R. E. Benveniste, R. Callahan, M. M. Lieber and C. J. Sherr 1159
Activation and Repression of Virus Expression in Mammalian Cells Infected by Rous Sarcoma Virus D. Boettiger 1169
Transformed Tumor Cell Surfaces
The Chick Fibroblast Cell Surface after Transformation by Rous Sarcoma Virus P. W. Robbins, G. G. Wickus, P. E. Branton, B. J. Gaffney, C. B. Hirschberg, P. Fuchs and P. M. Blumberg 1173
The Role of Cell Surface Changes in RNA Tumor Virus-transformed Cells H. Bauer, R. Kurth, L. Rohrschneider, G. Pauli, R. R. Friis and H. Gelderblom 1181
Summary
Tumor Viruses: 1974 D. Baltimore 1187
Symposium Participants v
Foreword xvii
Opening Remarks
Oncogenic Viruses: The Last Twelve Years R. Dulbecco 1
SV40 AND POLYOMA
Genome Structure and Genetics
Altered Patterns of Protein Synthesis in Infection by SV40 Mutants P. Tegt meyer 9
The Semiautonomous Replicon: A Molecular Model for the Oncogenicity of SV40 R. G. Martin, J. Y. Chou, J. Avila and R. Saral 17
Transformation of Primate and Rodent Cells by Temperature-sensitive Mutants of SV40 J. S. Butel, J. S. Brugge and C. A. Noonan 25
Properties of Temperature-sensitive Mutants of Polyoma Virus W. Eckhart 37
Indirect Complementation of a Nontransforming Mutant of Polyoma Virus T. L. Benjamin and E. Goldman 41
Polyoma Virus - A Study of Wild-type, Mutant and Defective DNAs M. Fried, B. E. Griffin, E. Lund and D. L. Robberson 45
Mapping the Genes of Simian Virus 40 C..J. Lai and D. Nathans 53
Mapping of Mutational Alterations in DNA with Si Nuclease: The Location of Deletions, Insertions and Temperature-sensitive Mutations in SV40 T. E. Shenk, C. Rhodes, P. W. J. Rigby and P. Berg 61
Isolation and Characterization of Individual Clones of Simian Virus 40 Mutants Containing Deletions, Duplications and Insertions in Their DNA J. E. Mertz, J. Carbon, M. Herzberg, R. W. Davis and P. Berg 69
Characterization of Defective Simian Virus 40 DNA: Comparison between Large- plaque and Small-plaque Types K. Yoshiike, A. Furuno, S. Watanabe, S. Uchida, K. Matsubara and Y. Takagi 85
Characterization of SV40 DNA Rescued from Transformed Mouse Cells M. Botchan, B. Ozanne and J. Sambrook 95
Host Substitution in SV40 and Polyoma DNA E. Winocour, N. Frenkel, S. Lavi, M. Osenholts and S. Rozenblatt 101
Electron Microscopic Mapping of the Attachment Sites on SV40 DNA during Lytic Infection L. T. Chow, H. W. Boyer, E. G. Tischer and H. M. Goodman 109
Characterization of Cloned Evolutionary Variants of Simian Virus 40 W. W. Brockman, T. N. H. Lee and D. Nathans 119
Specific Reiteration of Viral DNA Sequences in Mammalian Cells M. A. Martin, G. Khoury and G. C. Fareed 129
Formation of Reiterated Simian Virus 40 DNA D. Davoli and G. C. Fareed 137
The Detection and Quantitation of SV40 Nucleic Acid Sequences Using Single- stranded SV40 DNA Probes G. Khoury, P. Howley, M. Brown and M. Martin 147
RNA Transcription
Nucleotide Sequence about the 3¢ Terminus of SV40 DNA Transcripts and the Region Where DNA Synthesis Is Initiated R. Dhar, K. Subramanian, B. S. Zain, J. Pan and S. M. Weissman 153
Mapping of SV40 mRNA Species on the Viral Genome R. A. Weinberg and J. E. Newbold 161
Biogenesis and Characterization of SV40 and Polyoma RNAs in Productively Infected Cells Y. Aloni 165
Approaches to the Sequence Determination of SV40 DNA W. Fiers, K. Danna, R. Rogiers, A. Vandevoorde, J. Van Herreweghe, H. Van Heuverswyn, G. Volckaert and R. Yang 179
Virus-specific RNA in Cells Productively Infected or Transformed by Polyoma Virus R. Kamen, D. M. Lindstrom, H. Shure and R. W. Old 187
Replication
Stepwise Relaxation of Supercoiled SV40 DNA W. Keller and I. Wendel 199
Properties of Replicating SV40 DNA Molecules and Mapping Unpaired Regions in SV40 DNA I N. P. Salzman, J. Lebowitz, M. Chen, E. Sebring and C. F. Garon 209
Polyoma DNA Replication: Location of the Origin in Different Virus Strains L. V. Crawford, A. K. Robbins, P. M. Nicklin and K. Osborn 219
Replication of Polyoma DNA: Effects of Hydroxyurea and Arabinosyl Cytosine G. Magnusson, R. Craig, M. Närkhammar, P. Reichard, M. Staub and H. War- ner 227
Proteins and Their Synthesis
Fingerprints of Polyoma Virus Proteins and Mouse Histones G. Fey and B. Hirt 235
Virion Proteins of Polyoma Temperature-sensitive Mutants: Late Mutants T. Friedmann and W. Eckhart 243
Endonucleolytic Cleavage of Polyoma Virus DNA: General Properties and Site Specificity of the Virion-associated Endonuclease A. Parodi, P. Rouget, O. Croissant, D. Blangy and F. Cuzin 247
Intermolecular Disulfide Bonds: An Important Structural Feature of the Polyoma Virus Capsid G. Walter and W. Deppert 255
The Organization of Proteins in Polyoma and Cellular Chromatin A. J. Louie 259
SV40: T Antigen, the A Function and Transformation M. Osborn and K. Weber 267
Regulation of 5V40 Tumor Antigen in Transformed Mouse 3T3 Cells J. A. Robb 277
SV40 T Antigen: Partial Purification and Properties D. M. Livingston, I. C. Henderson and J. Hudson 283
Dissociation and DNA Binding of SV40 T Antigen R. B. Carroll, L. Hager and R. Dulbecco 291
An Antigen Associated with Messenger RNA in a Transformed Hamster Cell Line M.-E. Mirault, S. I. Reed and G. R.Stark 295
Cytosine Arabinoside- and Interferon-mediated Control of Polyoma and SV40 Genome Expression G. Brandner and N. Mueller 305
The Cell-free Translation of SV40 Messenger RNA C. L. Prives, H. Aviv, E. Gil- boa, M. Revel and E. Winocour 309
Transformed Cells
The Stable Classes of Transformed Cells Induced by SV40 Infection of Esta- blished 3T3 Cells and Primary Rat Embryonic Cells R. Risser, D. Ri/kin and R. Pollack 317
Factors Affecting the Process and Extent of Integration of the Viral Genome K. Hirai and V. Defendi 325
Genetics of Cell Transformation by Simian Virus 40 C. M. Croce, K. Huebner, A. J. Girardi and H. Koprowski 335
Variants of Simian Virus 40-transformed Mouse Cells Resistant to Cytochalasin B F. Kelly and J. Sambrook 345
The SV40 Rescue Problem J. F. Watkins 355
Localization and Distribution of Actin Fibers in Normal, Transformed and Re- vertant Cells K. Weber, E. Lazarides, R. D. Goldman, A. Vogel and R. Pol- lack 363
Differential Susceptibility to Amphotericin B of 3T3 Cells Transformed by SV40 or Polyoma P. Amati and C. Lago 371
Infection of Mouse Blastocysts with SV40 DNA: Normal Development of the In- fected Embryos and Persistence of SV40-specific DNA Sequences in the Adult Animals R. Jacnisch 375
A Simplifying Concept in Tumor Virology: Virus-specific "Pleiotropic Effectors" R. Weil, C. Salomon, E. May and P. May 381
ADENO VIRUSES
Genome Structure and Genetics
Cleavage Maps of DNA from Adenovirus Types 2 and 5 by Restriction Endo- nucleases EcoRI and HpaI C. Mulder, J. R. Arrand, H. Delius, W. Keller, U. Pettersson, R. J. Roberts and P. A. Sharp 397
Extent of Terminal Repetition in Adenovirus 2 DNA J. R. Arrand, W. Keller and R. J. Roberts 401
Structural Studies on Two Adenovirus 2-SV40 Hybrids Which Contain the En- tire 5V40 Genome T. J. Kelly, Jr., A. M. Lewis, Jr., A. S. Levine and S. Siegel 409
Cell Transformation: A Study of Regulation with Types S and 12 Adenovirus Temperature-sensitive Mutants H. S. Ginsberg, M. J. Ensinger, R. S. Kauff- man, A. J. Mayer and U. Lundholm 419
Genetic Analysis of Human Adenovirus Type 5 in Permissive and Nonpermissive Cells J. F. Williams, C. S. H. Young and P. E. Austin 427
Physical Mapping of Temperature-sensitive Mutations of Adenoviruses T. Grodzicker, J. Williams, P. Sharp and J. Sambrook 439
Transcription
RNA Synthesis and Processing in Adenovirus-infected Cells L. Philipson, U. Pettersson, U. Lind berg, C. Tibbetts, B. Vennstrom and T. Persson 447
Mapping of Adenovirus 2 RNA Sequences in Lytically Infected Cells and Trans- formed Cell Lines P. A. Sharp. P. H. Gallimore and S. J. Flint 457
An Examination of the Nuclear RNA of Adenovirus-transformed Cells M. Gear- gieff S. Bachenheimer and J. E. Darnell 475
RNA Transcription in Cultures Productively Infected with Adenovirus 2 E. A. Craig, J. Tal, T. Nishimoto, S. Zimmer, M. MeGrogan and H. J. Raskas 483
The Transcriptional Role of Host DNA-dependent RNA Polymerases in Adeno- virus-infected KB Cells R. Weinmann, H. J. Raskas and R. G. Roeder 495
In Vitro Transcription of Adenovirus 2 DNA byE. coli RNA Polymerase S. Sur- zycki, J. A. Surzycki, W. DeLorbe and G. N. Gussin 501
Replication
Integration of Adenovirus DNA into the Cellular Genome W. Doer/ler, H. Bur- ger, J. Ortin, E. Fanning, D. T. Brown, M. Westphal, U. Winterhoff, B. Weiser and J. Schick 505
A Circular DNA-Protein Complex from Adenoviruses and Its Possible Role in DNA Replication A. J. Robinson and A. J. D. Bellett 523
The Viral DNA Replication Machinery of Adenovirus 12 H. Shimojo, K. Shiroki and K. Yamaguchi 533
The Mechanism of Replication of Adenovirus Type 5 DNA J. S. Sussenbach, D. J. Ellens, P. Ch. van der Vliet, M. G. Kuijk, P. H. Steenbergh, J. M. VIak, Th. H. RozUn and H. S. Jansz 539
Origins and Termini of Adenovirus Type 2 DNA Replication E. L. Winnacker 547
Proteins and Their Synthesis
Early Events in Adenovirus-infected Cells W. C. Russell 551
Adenovirus-infected, Cell-specific, DNA-binding Proteins A. J. Levine, P. C. van der Vlict, B. Rosenwirth, J. Rabek, G. Frenkel and M. Ensinger 559
Human Adenovirus Infection in Monkey Cells: An Example of Host Restriction at a Step Late in Replication S. G. Baum and R. I. Fox 567
Analysis of Adenovirus Type 2 Gene Functions by Cell-free Translation of Viral Messenger RNA H. Westphal, L. Eron, F.-J. Ferdinand, R. Callahan and S-P. Lai 575
The Origin and Destiny of Adenovirus Proteins J. B. Lewis, C. W. Anderson, J. F. Atkins and R. F. Gesteland 581
Transformed Cells
Recent Studies on the Characteristics of Adenovirus-infected and -transformed Cells J. K. McDougall, A. R. Dunn and P. H. Gallimore 591
Properties and Behavior of Hamster Embryo Cells Transformed by Human Ad- enovirus Type 5 R. D. Goldman, C. Chang and J. F. Williams 601
Viral DNA Sequences in Cells Transformed by Simian Virus 40, Adenovirus Type 2 and Adenovirus Type 5 J. Sambrook, M. Botchan, P. Gallimore, B. Ozanne, U. Pettersson, J. Williams and P. A. Sharp 615
Analysis of Multiple Viral Genome Fragments in Adenovirus 7-transformed Hamster Cells K. Fujinaga, K. Sekikawa, H. Yamazaki and M. Green 633
Studies on In Vitro Transformation by DNA and DNA Fragments of Human Adenoviruses and Simian Virus 40 F. L. Graham, P. J. Abrahams, C. Mul- der, H. L. He~neker, 5. 0. Warnaar, F. A. J. de Vries, W. Fiers and A. J. van der Eb 637
Studies of Hamster Cells Transformed by Adenovirus 2 and the Nondefective Ad2-SV40 Hybrids A. M. Lewis, Jr., J. H. Breeden, Y. L. Wewerka, L. E. Schnipper and A. S. Levine 651
PART 2
HERPES VIRUSES
Herpes Simplex
The Structure and Biological Properties of Herpes Simplex Virus DNA N. M. Wilkie, J. B. Clements, J. C. M. Macnab and J. H. Subak-Sharpe 657
Inverted Repetitions in the Chromosome of Herpes Simplex Virus P. Sheldrick and N. Berthelot- 667
Terminal Repetitions in Herpes Simplex Virus Type 1 DNA R. H. Grafstrom, J. C. Alwine, W. L. Steinhart and C. W. Hill 679
Analysis of DNA of Defective Herpes Simplex Virus Type 1 by Restriction Endo- nuclease Cleavage and Nucleic Acid Hybridization M. Wagner, J. Share and W. C. Summers 683
Regulation of Herpesvirus Macromolecular Synthesis: Evidence for Multilevel Regulation of Herpes Simplex 1 RNA and Protein Synthesis B. Roizman, M. Kozak, R. W. lioness and G. Hayward 687
Thymidine Kinase Isozymes of Normal and Virus-infected Cells S. Kit, W.-C. Leung, G. N. Jorgensen, D. Trkula and D. R. Dubbs 703
Genetic and Biochemical Studies with Herpesvirus J. H. Subak-Sharpe, S. M. Brown, D. A. Ritchie, M. C. Timbu,y, J. C. M. Macnab, H. S. Marsden and J. Hay 717
Viral Gene Functions Expressed and Detected by Temperature-sensitive Mutants of Herpes Simplex Virus M. Benyesh-Melnick, P. A. Schaffer, R. J. Courtney, J. Esparza and S. Kimura 731
Demonstration of the Oncogenic Potential of Herpes Simplex Viruses and Human Cytomegalovirus F. Rapp and J.-L. H. Li 747
Transformation of Cells by Herpes Simplex Virus - Fact or Fantasy? B. R. Mc- Auslan, B. Gar/inkle, R. Adler, D. Devinney, R. Florkiewicz and J. E. Shaw 765
Epstein-Barr Virus
Immortalizing and Nonimmortalizing Laboratory Strains of Epstein-Barr Virus G. Miller, J. Robinson and L. Heston 773
Studies on the Epstein-Barr Virus Genome and the EBV-determined Nuclear Antigen in Human Malignant Disease G. Klein 783
EBV DNA in Nonlymphoid Cells of Nasopharyngeal Carcinomas and in a Malig- nant Lymphoma Obtained after Inoculation of EBV into Cottontop Marmo- sets H. Wolf J. Werner and H. zur Hausen 791
The Epstein-Barr Virus and Malignancy: Molecular Evidence J. S. Pagano 797
Plasmid DNA as a Possible State of Epstein-Barr Virus Genomes in Nonproduc- tive Cells M. Nonoyama and A. Tanaka 807
Sequence of Epstein-Barr Virus Productive Cycle in Human Lymphoblastoid Cells B. Hampar, J. G. Derge, A. Tanaka and M. Nonoyama 811
Response of B and T Lymphocytes and Other Blood Leukocytes in Chickens with Marek's Disease L. N. Payne, P. C. Powell and M. Rennie 817
Organization of Virion RNA Genome and Genetics
Electron Microscope Studies of Tumor Virus RNA H. J. Kung, J. M. Bailey, N. Davidson, P. K. Vogt, M.O. Nicolson and R. M. McAllister 827
Electron Microscope Measurements of Rous Sarcoma Virus RNA H. Delius, P. H. Duesberg and W. F. Mangel 835
Determination of the Molecular Weight of the RNA Subunits of Rous Sarcoma Virus by Electron Microscopy A. B. Jacobson and P. A. Bromley 845
Avian RNA Tumor Viruses: Mechanism of Recombination and Complexity of the Genome P. Duesberg, P. K. Vogt, K. Beemon and M. Lai 847
35S a and b RNA Subunits of Avian RNA Tumor Virus Strains Cloned and Pas- saged in Chick and Duck Cells M. P. Stone, R. E. Smith and W. K. ,Joklik 859
Base Sequence Complexity of 35S Avian Myeloblastosis Virus RNA Determined by Molecular Hybridization Kinetics M. A. Baluda, M. Shoyab, P. D. Mark- ham, R. M. Evans and W. N. Drohan 869
Studies of the Interrelationship of Chicken Leukosis Virus and Host Cell Genomes by RNA-DNA Hybridization P. E. Neiman, S. E. Wright and H. G. Purchase 875
A Biochemical and Genetic Analysis of Mammalian RNA-containing Sarcoma Viruses E. M. Scolnick, R. J. Goldberg and W. P. Parks 885
Genetic Recombination among Temperature-sensitive Mutants of Rous Sarcoma Virus J. A. Wyke, J. G. Bell and J. A. Beamand 897
Phenotypically Mixed Vesicular Stomatitis Virus Particles Produced in Human Tumor Cell Lines J. Zavada, J. Bubenik, R. Widmaicr and Z. Zavadova 907
Phenotypic Mixing between Vesicular Stomatitis Virus and Avian RNA Tumor Viruses R. A. Weiss, D. Boettiger and D. N. Love 913
Reverse Transcriptase
Role of the Subunits of the Avian RNA Tumor Virus Reverse Transcriptase A. Panet, I. M. Verma and D. Baltimore 919
Structure and Properties of an RNA Primer for Initiation of Rous Sarcoma Virus DNA Synthesis In Vitro J. E. Dahlberg, F. Harada and R. C. Sawyer 925
Relationships between Components in Primate RNA Tumor Viruses and in the Cytoplasm of Human Leukemic Cells: Implications to Leukemogenesis R. C. Gallo, R. E. Gallagher, N. R. Miller, H. Mondal, W. C. Saxinger, R. J. Mayer, R. G. Smith and D. H. Gillespie 933
Reverse Transcriptase Associated with A-type Particles from Murine Myeloma Cells R. E. Thach, D. L. Robertson, N. L. Baenziger and D. C. Dobbertin 963
Reverse Transcriptase and RNase H: Present in a Murine Virus and in Both Sub- units of an Avian Virus K. Moelling 969
Proviral DNA and Its Transcription
Properties of Oncornavirus RNA-directed DNA Polymerase, the RNA Template, and the Intracellular Products Formed Early during Infection and Cell Transformation M. Green, D. Grandgenett, G. Gerard, H. M. Rho, M. C. Loni, M. Robins, S. Salzberg, G. Shanmugam, S. Bhaduri and G. Vecchio 975
Synthesis, Structure and Function of Avian Sarcoma Virus-specific DNA in Permissive and Nonpermissive Cells H. E. Varmus, R. V. Guntaka, C. T. Deng and J. M. Bishop 987
The Genome of RNA Tumor Viruses: A Functional Requirement for a Polyploid Structure? P. D. Cooper and J. A. Wyke 997
Studies on Characterization of the Integration Sites of Avian RNA Tumor Virus- specific DNA R. M. Evans, M. Shoyab and M. A. Baluda 1005
DNA-RNA Hybrid in Cells Infected by Murme Leukemia Virus T. Takano and M. Hatanaka 1009
Infectious Viral DNA in Rous Sarcoma Virus-transformed Nonproducer and Producer Animal Cells M. Hill, J. Hillova, D. Dantchev, R. Manage and G. Goubin 1015
Infectious DNA from Cells Infected with Rous Sarcoma Virus, Reticulcendothe- liosis Virus or Rous-associated Virus-0 G. M. Cooper and H. M. Temin 1027
AMV RNA Transcription in Cell-free Systems and Properties of In Vitro Chro- matin-directed RNA Synthesis G. Monroy, M. Jacquet, Y. Groner and J. Hurwitz 1033
Studies on the Structure and Synthesis of Rous Sarcoma Virus RNA C. Weiss- mann, J. T. Parsons, J. W. Coffin, L. Rymo, M. A. Billeter and H. Hofstetter 1043
Proteins
Characterization of Murine Leukemia Virus-specific Proteins E. Fleissner, H. Ikeda, J-S. Tung, E. S. Vitetta, E. Tress, W. Hardy, Jr., E. Stockert, E. A. Boyse, T. Pincus and P. O'Donnell 1057
Synthesis of Avian RNA Tumor Virus Structural Proteins R. Eisenman, V. M. Vogt and H. Diggelmann 1067
Expression of Endogeneous Viruses
Problems of RSV Rescue from Virogenic Mammalian Cells J. Svododa, I. Hlo žánek, O. Mach and S. Zadražil 1077
Qualitative and Quantitative Studies of AKR-type Murine Leukemia Virus Sequences in Mouse DNA S. K. Chattopadhyay, D. R. Lowy, N. M. Teich, A. S. Levine and W. P. Rowe 1085
Mechanism of Induction of RNA Tumor Viruses by Halogenated Pyrimidines P. Besmer, D. Smotkin, W. Haseltine, H. Fan, A. T. Wilson, M. Paskind, R. Weinberg and D. Baltimore 1103
Structural Proteins of RNA Tumor Viruses as Probes for Viral Gene Expression M. Strand and J. T. August 1109
Gen etic Control of the Expression of Murine Leukemia Virus Proteins in Tissues of Normal, Young Adult Mice M. Strand, F. Lilly and J. T. August 1117
Immune Response in the Mouse to Endogenous Leukemia Viruses R. C. Nowin- ski, S. L. Kaehler and R. R. Burgess 1123
Differential Cellular Regulation of Three Distinct Classes of Type C RNA Viruses Endogenous to Mouse Cells S. A. Aaronson and J. R. Stephenson 1129
Control of Expression of Tumor Virus Genes in Uninfected Chicken Cells H. Hanaf~tsa, W. S. Hayward, J. H. Chen and T. Hanafusa 1139
Endogenous Mammary Tumor Viruses in Mice P. Bentvelzen 1145
Glucocorticoid Induction of Murine Mammary Tumor Virus In Vitro W. P. Parks, E. M. Scolnick and J. C. Ransom 1151
Endogenous Primate and Feline Type C Viruses G. J. Todaro, R. E. Benveniste, R. Callahan, M. M. Lieber and C. J. Sherr 1159
Activation and Repression of Virus Expression in Mammalian Cells Infected by Rous Sarcoma Virus D. Boettiger 1169
Transformed Tumor Cell Surfaces
The Chick Fibroblast Cell Surface after Transformation by Rous Sarcoma Virus P. W. Robbins, G. G. Wickus, P. E. Branton, B. J. Gaffney, C. B. Hirschberg, P. Fuchs and P. M. Blumberg 1173
The Role of Cell Surface Changes in RNA Tumor Virus-transformed Cells H. Bauer, R. Kurth, L. Rohrschneider, G. Pauli, R. R. Friis and H. Gelderblom 1181
Summary
Tumor Viruses: 1974 D. Baltimore 1187
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