Plant Biology Newsfeeds

  • Calcium-dependent protein kinase CPK28 targets the methionine adenosyltransferases for degradation by the 26S proteasome and affects ethylene biosynthesis and lignin deposition in Arabidopsis

    The Plant Journal 23 Jan 2017 | 7:30 am

    Summary S-adenosylmethionine (AdoMet) is synthesized by methionine adenosyltransferase (MAT) and plays an essential role in ethylene biosynthesis and other methylation reactions. Despite increasing knowledge of MAT regulation at transcriptional levels, how MAT is post-translationally regulated remains unknown in plant cells. Phosphorylation is an important post-translational modification for regulating the activity of enzymes, protein function and signaling transduction. Using molecular and biochemical approaches, we have identified the phosphorylation of MAT proteins by calcium dependent protein kinase (CPK28). Phenotypically, both MAT2-overexpressing transgenic[…]

  • Orchestrating Rapid Long-Distance Signaling in Plants with Ca2+, ROS, and Electrical Signals

    The Plant Journal 23 Jan 2017 | 7:20 am

    Summary Plants show a rapid systemic response to a wide range of environmental stresses, where the signals from the site of stimulus perception are transmitted to distal organs to elicit plant-wide responses. A wide range of signaling molecules are trafficked through the plant, but a trio of potentially interacting messengers, reactive oxygen species (ROS), Ca2+ and electrical signaling (‘trio signaling’) appear to form a network supporting rapid signal transmission. The molecular components underlying this rapid communication are beginning to be[…]

  • Label-free Deep Shotgun Proteomics reveals Protein Dynamics during Tomato Fruit Tissues Development

    The Plant Journal 23 Jan 2017 | 7:15 am

    Abstract Current innovations in mass-spectrometry-based technologies allow deep coverage of protein expression. Despite its immense value and in contrast to transcriptomics, only a handful of studies in crop plants engaged with global proteome assays. Here, we present large-scale shotgun proteomics profiling of tomato fruit across two key tissues and five developmental stages. A total of 7738 individual protein groups were identified and reliably measured at least in one of the analyzed tissues or stages. The depth of our assay enabled[…]

  • Integrated omics analysis of specialized metabolism in medicinal plants

    The Plant Journal 21 Jan 2017 | 8:55 am

    Summary Medicinal plants are the rich source of highly diverse specialized metabolites with important pharmacological properties. Until recently, plant biologists were limited in their approach to explore biosynthetic pathways of these metabolites, mainly due to the scarcity of plant genomics resources. Recent advancements in the high-throughput large-scale analytical methods have enabled plant biologists to discover biosynthetic pathways for important plant-based medicinal metabolites. Reduced cost to generate omics datasets, and the development of computational tools for their analysis and integration have[…]

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